Engineering the l-Arabinose Isomerase from Enterococcus Faecium for d-Tagatose Synthesis [PDF]
Molecules, 2017 l-Arabinose isomerase (EC 5.3.1.4) (l-AI) from Enterococcus faecium DBFIQ E36 was overproduced in Escherichia coli by designing a codon-optimized synthetic araA gene. Using this optimized gene, two N- and C-terminal His-tagged-l-AI proteins were produced.
Marylane de Sousa +5 more
doaj +7 more sources
Expression and Characterization of L-Arabinose Isomerase and Its Enzymatic Recycling of the Expired Milk [PDF]
FoodsAs global milk production continues to rise, the disposal of expired milk contributes to environmental pollution and valuable resource wastage.
Zhou Chen +4 more
doaj +4 more sources
Exploring a Highly D-Galactose Specific L-Arabinose Isomerase From Bifidobacterium adolescentis for D-Tagatose Production [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 D-Galactose-specific L-arabinose isomerase (L-AI) would have much potential for the enzymatic conversion of D-Galactose into D-tagatose, while most of the reported L-AIs are L-arabinose specific.
Guoyan Zhang +5 more
doaj +2 more sources
Biochemical properties of L-arabinose isomerase from Clostridium hylemonae to produce D-tagatose as a functional sweetener. [PDF]
PLoS ONE, 2018 d-Tagatose has gained substantial interest due to its potential functionalities as a sucrose substitute. In this study, the gene araA, encoding l-arabinose isomerase (l-AI) from Clostridium hylemonae (DSM 15053), was cloned and expressed in Escherichia ...
Tien-Kieu Nguyen +4 more
doaj +2 more sources
The secreted L-arabinose isomerase displays anti-hyperglycemic effects in mice. [PDF]
Microb Cell Fact, 2015 The L-arabinose isomerase is an intracellular enzyme which converts L-arabinose into L-ribulose in living systems and D-galactose into D-tagatose in industrial processes and at industrial scales. D-tagatose is a natural ketohexose with potential uses in pharmaceutical and food industries.
Rhimi M +9 more
europepmc +7 more sources
Improving Catalytic Efficiency of L-Arabinose Isomerase from Lactobacillus plantarum CY6 towards D-Galactose by Molecular Modification [PDF]
FoodsL-Arabinose isomerase (L-AI) has been commonly used as an efficient biocatalyst to produce D-tagatose via the isomerization of D-galactose. However, it remains a significant challenge to efficiently synthesize D-tagatose using the native (wild type) L-AI
Chengyu Lu +8 more
doaj +2 more sources
Enabling Stable Recycling of L-Arabinose Isomerase Through Whole-Cell Immobilization for Efficient and Cost-Effective D-Tagatose Production [PDF]
FoodsD-tagatose is a functional sweetener with glucose-regulating and prebiotic properties, but its bioproduction from D-galactose faces many limitations, particularly the high production costs.
Zepeng Li +7 more
doaj +2 more sources
The acid-tolerant
Microbial Cell Factories, 2011 Background L-arabinose isomerases catalyse the isomerization of L-arabinose into L-ribulose at insight biological systems. At industrial scale of this enzyme is used for the bioconversion of D-galactose into D-tagatose which has many applications in ...
Rhimi Moez +6 more
doaj +2 more sources
Recombinant production of acidophilic L-arabinose isomerase from Lentilactobacillus parakefiri in Bacillus subtilis [PDF]
Microbial Cell FactoriesBackground The monosaccharide D-tagatose is a promising alternative to sucrose because of its similar sweetness and lower glycemic index. A novel L-arabinose isomerase (L-AI) from Lentilactobacillus parakefiri DSM 10551 (L-AI-Lp) has been biochemically ...
Nathanael Weber +4 more
doaj +2 more sources
Engineering mixed sugar metabolic channels in Pseudomonas putida to produce vanillic acid [PDF]
Synthetic and Systems BiotechnologyHydrolysis of lignocellulosic biomass produces the mixed sugars of glucose (60–70 %), xylose (20–30 %), and arabinose (2–20 %), etc. Using mixed sugars instead of pure glucose for microbial biosynthesis will reduce the cost of carbon source and maximize ...
Peihan Wu +6 more
doaj +2 more sources