Results 171 to 180 of about 6,201 (226)

Glucono-δ-lactonase from Escherichia coli

Biochimica et Biophysica Acta (BBA) - Enzymology, 1972
Escherichia coli K12 has been shown to contain a glucono-δ-lactonase (d-glucono-δ-lactone hydrolase, EC 3.1.1.17). A convenient assay has been developed which is based on the spectral change of a pH indicator during the hydrolysis of the lactone. The enzyme has been purified about 105-fold. Its substrate specificity has been investigated. The enzyme is
F, Hucho, K, Wallenfels
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Structural Basis for Natural Lactonase and Promiscuous Phosphotriesterase Activities

Journal of Molecular Biology, 2008
Organophosphates are the largest class of known insecticides, several of which are potent nerve agents. Consequently, organophosphate-degrading enzymes are of great scientific interest as bioscavengers and biodecontaminants. Recently, a hyperthermophilic phosphotriesterase (known as SsoPox), from the Archaeon Sulfolobus solfataricus, has been isolated ...
Mikael H Elias, Jerome Dupuy, Luigi Mandrich   +2 more
exaly   +5 more sources

Preparation and properties of a steroid lactonase

Archives of Biochemistry and Biophysics, 1965
Abstract A steroid-lactonase has been obtained from disrupted cells of Cephalosporium acremonium . Properties of the purified enzyme are described. Testololactone derivatives are hydrolyzed by the enzyme, but steroids containing γ-lactones, e.g., digitoxigenin and a spirolactone, are not.
C E, HOLMLUND, R H, BLANK
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Enzymes with Phosphotriesterase and Lactonase Activities in Archaea

Current Chemical Biology, 2008
Organophosphates (OPs) are a class of synthetic compounds used both as pesticides and nerve agents. They are chemically phosphoesters and biologically potent irreversible inhibitors of serine esterases, particularly acetylcholinesterase (ACh), the key enzyme of the nervous system.
Merone L, Mandrich L, Rossi M, Manco G
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PON1 lactonase activity and its association with cardiovascular disease

Clinica Chimica Acta, 2020
Paraoxonase 1 (PON1) is important in the development of atherosclerosis, and it has become the subject of intensive research. Our aim was to evaluate the association of serum PON1 activity and polymorphisms with cardiovascular disease (CVD) using four different substrates.Activity of PON1-related to arylesterase (AREase and 4-CMPAse), paraoxonase ...
F.E. Murillo-González, N. Ponce-Ruiz, A.E. Rojas-García, S.J. Rothenberg, Y.Y. Bernal-Hernández, R.M. Cerda-Flores, Mike Mackness, B.S. Barrón-Vivanco, C.A. González-Arias, J. Ponce-Gallegos, I.M. Medina-Díaz   +10 more
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[60] Actinomycin lactonase

1975
Publisher Summary This chapter discusses the assay and purification procedure of actinomycin lactonase. The enzyme catalyzes the conversion of a neutral molecule to an acidic compound. The assay is based upon the use of radioactive actinomycin. Both product and substrate are extracted from the reaction mixture with an organic solvent at an acid pH ...
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[61] Peptide antibiotic lactonase

1975
Publisher Summary This chapter discusses the assay and purification procedure of peptide antibiotic lactonase. .The enzyme catalyzes the conversion of a neutral molecule to an acidic compound. The assay is based on the use of radioactive substrate or non-labeled material.
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Hyperthermophilic phosphotriesterases/lactonases for the environment and human health

Environmental Technology, 2010
In the last decades the idea to use enzymes for environmental bioremediation has been more and more proposed and, in the light of this, new solutions have been suggested and detailed studies on some classes of enzymes have been performed. In particular, our attention in the last few years has been focused on the enzymes belonging to the amidohydrolase ...
Mandrich L, Merone L, Manco G
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Lactonases with oragnophosphatase activity: Structural and evolutionary perspectives

Chemico-Biological Interactions, 2010
Serum paraoxonase (PON1) is well recognized for its ability to hydrolyze arylesters, toxic oxon metabolites of organophosphate insecticides and nerve agents. PON1 is a member of gene family including also PON2 and PON3; however, the later two enzymes have very limited arylesterase and practically no organophosphatase activity.
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Studies on the enantioselectivity of bacterial lactonases

2022
Owing to their biological significance, increased efforts are being directed towards the production of lactones in optically pure form. One method of synthesis is via a microbial equivalent of the Baeyer-Villiger reaction. This reaction is common in microorganisms possessing a monooxygenase enzyme which is induced as part of the catabolic machinery ...
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