Results 81 to 90 of about 92,845 (317)

Structural basis for lamin assembly at the molecular level

Nature Communications, 2019
Nuclear structure and function are governed by lamins, which are intermediate filaments that mostly consist of α-helices. Different lamin assembly models have been proposed based on low resolution and fragmented structures.
Jinsook Ahn, I. Jo, So-mi Kang, Seokho Hong, Suhyeon Kim, Soyeon Jeong, Yong-Hak Kim, Bum-Joon Park, N. Ha   +8 more
semanticscholar   +1 more source

The Floor‐Ceiling‐Chip, or 2 × 2D = Pseudo‐3D—Approaching 3D Cell Morphology and Organization between Two Opposing 2D Substrates with Cell‐Adhesive Protein Micropatterns

Advanced Healthcare Materials, EarlyView.
Here, we present a novel 3D cell patterning and culture platform. The “Floor‐Ceiling‐Chip” (FC‐Chip) consists of two opposing track‐etched membranes, creating a pseudo‐3D microenvironment for the cells in between. By providing the membranes with micropatterned cell‐adhesive islands of varying geometries and sizes, the FC‐Chip enables control over cell ...
Urandelger Tuvshindorj, Francesca Giacomini, Esra Güben Kaçmaz, Tim ten Brink, Lorenzo Moroni, Zeinab Tahmasebi Birgani, Clemens van Blitterswijk, Pamela Habibović, Stefan Giselbrecht, Jan de Boer, Roman Truckenmüller   +10 more
wiley   +1 more source

A Lamin Family-Based Signature Predicts Prognosis and Immunotherapy Response in Hepatocellular Carcinoma

Journal of Immunology Research, 2022
Background. Lamin family members play crucial roles in promoting oncogenesis and cancer development. The values of lamin family in predicting prognosis and immunotherapy response remain largely unclarified.
Yongyu Yang, Wang Xiao, Ruoqi Liu, Lei Gao, Junzhang Chen, Heping Kan   +5 more
doaj   +1 more source

Drosophila nuclear lamin precursor Dm0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene. [PDF]

, 1988
A cDNA clone encoding a portion of Drosophila nuclear lamins Dm1 and Dm2 has been identified by screening a lambda-gt11 cDNA expression library using Drosophila lamin-specific monoclonal antibodies.
Bare, JW, Benton, BM, Drees, B, Fisher, PA, Gruenbaum, Y, Landesman, Y, Paddy, MR, Saumweber, H, Sedat, JW, Smith, DE   +9 more
core   +1 more source

Structure and stability of the lamin A tail domain and HGPS mutant [PDF]

, 2011
Hutchinson–Gilford progeria syndrome (HGPS) is a premature aging syndrome caused by the expression and accumulation of a mutant form of lamin A, Δ50 lamin A.
Buehler, Markus J, Dahl, Kris Noel, Kalinowski, Agnieszka, Qin, Zhao   +3 more
core   +1 more source

Common Signatures of Altered Gene Regulation and Invasiveness of Different Breast Cancer Cell Lines after Matrix Interface Crossing

Advanced Healthcare Materials, EarlyView.
Interface transmigration reprograms triple‐negative breast cancer cells, triggering a shared switch toward more aggressive and invasive phenotypes. Using a collagen I interface model, this study identifies shared transcriptional changes involving proliferation, chromatin remodeling, and DNA repair pathways.
Cornelia Clemens, Hannah Trampert, Nataliia Kotsiuba, Tilo Pompe   +3 more
wiley   +1 more source

Chromosome positioning is largely unaffected in lymphoblastoid cell lines containing emerin or A-type lamin mutations [PDF]

, 2005
Gene-poor human chromosomes are reproducibly found at the nuclear periphery in proliferating cells. There are a number of inner nuclear envelope proteins that may have roles in chromosome location and anchorage, e.g. emerin and A-type lamins.
Bridger, JM, Levy, N, Meaburn, KJ, Toniolo, D   +3 more
core   +1 more source

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications

Advanced Science, EarlyView.
Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, Raymond J. Monnat, Ross C. Wilson, Benjamin S. Freedman   +12 more
wiley   +1 more source

Use of Farnesyl Transferase Inhibitors in an Ageing Model in Drosophila

Journal of Developmental Biology, 2023
The presence of farnesylated proteins at the inner nuclear membrane (INM), such as the Lamins or Kugelkern in Drosophila, leads to specific changes in the nuclear morphology and accelerated ageing on the organismal level reminiscent of the Hutchinson ...
Annely Brandt, Roman Petrovsky, Maria Kriebel, Jörg Großhans   +3 more
doaj   +1 more source

Chrom3D: three-dimensional genome modeling from Hi-C and nuclear lamin-genome contacts

Genome Biology, 2017
Current three-dimensional (3D) genome modeling platforms are limited by their inability to account for radial placement of loci in the nucleus. We present Chrom3D, a user-friendly whole-genome 3D computational modeling framework that simulates positions ...
Jonas Paulsen, M. Sekelja, Anja R. Oldenburg, Alice Barateau, N. Briand, E. Delbarre, Akshay Shah, Anita L. Sørensen, C. Vigouroux, B. Buendia, P. Collas   +10 more
semanticscholar   +1 more source