Results 41 to 50 of about 21,610 (241)

LC-HRMS and GC-MS Profiling of Urine Free Cortisol, Cortisone, 6Β-, and 18-Hydroxycortisol for the Evaluation of Glucocorticoid and Mineralocorticoid Disorders

Biomolecules
Introduction: Urine free cortisol measurements are routinely performed to evaluate hypercortisolism. Despite their analytical inaccuracy, immunoassay-based methods are frequently used.
Gregori Casals, María Antonieta Ballesteros, Angielys Zamora, Irene Martínez, Guillermo Fernández-Varo, Mireia Mora, Felicia A. Hanzu, Manuel Morales-Ruiz   +7 more
doaj   +1 more source

Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry

Toxins, 2019
Routine mycotoxin biomonitoring methods do not include many mycotoxin phase I and phase II metabolites, which may significantly underestimate mycotoxin exposure especially for heavily metabolized mycotoxins. Additional research efforts are also needed to
Irina Slobodchikova, Reajean Sivakumar, Md Samiur Rahman, Dajana Vuckovic   +3 more
doaj   +1 more source

Metabolism Profile of Mequindox in Sea Cucumbers In Vivo Using LC-HRMS

Antibiotics, 2022
In this work, the metabolism behavior of mequindox (MEQ) in sea cucumber in vivo was investigated using LC-HRMS. In total, nine metabolites were detected and identified as well as the precursor in sea cucumber tissues. The metabolic pathways of MEQ in sea cucumber mainly include hydrogenation reduction, deoxidation, carboxylation, deacetylation, and ...
Xin Mao, Xiaozhen Zhou, Jun He, Gongzhen Liu, Huihui Liu, Han Zhao, Pengjie Luo, Yongning Wu, Yanshen Li   +8 more
openaire   +3 more sources

Metabologenomic Hallmark‐Based Discovery of Bacterial Thioamides as a New Lead against Drug‐Resistant Pancreatic Cancer

Advanced Science, EarlyView.
A targeted metabologenomic strategy integrating genomic and metabolomic hallmarks enabled the discovery of two new thioamides, thiogochangamides A and B, from bacteria. Their absolute configurations, previously unresolved within the thioviridamide family, were fully assigned through various chemical derivatizations and computational analysis ...
Young Eun Du, Eun Seo Bae, Thinh T. M. Bui, Seok Beom Lee, Dahan Kim, Jin‐Gyeong Park, Yongjoo Park, Soo Yeon Park, Sangwook Kang, Beomsu Lee, Daniel Shin, Yun Pyo Kang, In‐Gyun Lee, Dae‐Duk Kim, Seokhee Kim, Suckchang Hong, Kyuho Moon, Sang Kook Lee, Dong‐Chan Oh   +18 more
wiley   +1 more source

Novel sulphur-containing imatinib metabolites found by untargeted LC-HRMS analysis

European Journal of Pharmaceutical Sciences, 2017
Untargeted metabolite profiling using high-resolution mass spectrometry coupled with liquid chromatography (LC-HRMS), followed by data analysis with the Compound Discoverer 2.0™ software, was used to study the metabolism of imatinib in humans with chronic myeloid leukemia.
Ivo Vrobel, David Friedecký, Edgar Faber, Lukáš Najdekr, Kateřina Mičová, Radana Karlíková, Tomáš Adam   +6 more
openaire   +2 more sources

Development and Structural Characterization of UTE‐156, a Covalent Inhibitor of the VCP/p97 AAA+ ATPase

Advanced Science, EarlyView.
The AAA+ ATPase Valosin‐containing protein (VCP/p97) regulates protein homeostasis by unfolding ubiquitinated substrates. Here, we describe UTE‐156, a novel irreversible covalent inhibitor that modifies Cys522 in the D2 ATPase motor domain. Although its pharmacochemical limitations preclude immediate therapeutic use, UTE‐156 serves as a valuable ...
Daniela Tamayo‐Jaramillo, Subramanya Hegde, Xuan Jia, Kimberly Coffman, Hariprasad Vankayalapati, David Bearss, Kevin B. Jones, Alex W. Stark, Peter S. Shen   +8 more
wiley   +1 more source

Isolation and Characterization of [D-Leu¹]microcystin-LY from Microcystis aeruginosa CPCC-464

Toxins, 2020
[D-Leu1]MC-LY (1) ([M + H]+ m/z 1044.5673, Δ 2.0 ppm), a new microcystin, was isolated from Microcystis aeruginosa strain CPCC-464. The compound was characterized by 1H and 13C NMR spectroscopy, liquid chromatography−high resolution tandem ...
Patricia LeBlanc, Nadine Merkley, Krista Thomas, Nancy I. Lewis, Khalida Békri, Susan LeBlanc Renaud, Frances R. Pick, Pearse McCarron, Christopher O. Miles, Michael A. Quilliam   +9 more
doaj   +1 more source

Characterization of an Amphetamine Interference from Gabapentin in an LC–HRMS Method [PDF]

Journal of Analytical Toxicology, 2019
Abstract An amphetamine interference was observed during the development of an liquid chromatography–high-resolution mass spectrometry (LC–HRMS) multi-class confirmation method for the determination of 47 drugs and metabolites in urine. The interference passed all qualitative criteria for amphetamine leading to potential false-positive ...
Ana Celia, Muñoz-Muñoz, Teresa, Pekol, Dana, Schubring, Robin, Hyland, Charlene, Johnson, Lawrence, Andrade   +5 more
openaire   +2 more sources

Multi‐Targeting Non‐Specific Genome Engineering in Bacteria

Advanced Science, EarlyView.
In this study, we provide the first case to use the multi‐targeting integrase (MTI) systems in bacteria and develop a host‐independent generalizable approach, MNGE (Multi‐targeting Non‐specific Genome Engineering), for multi‐copy and random integration of metabolic genes or pathways in both Gram‐positive and Gram‐negative bacteria, which will ...
Runze Sun, Ruixiang You, Yiwen Zhou, Huiyan He, Wenfang Wang, Xudong Qu, Yinhua Lu, Lei Li   +7 more
wiley   +1 more source

Development, Validation, and Comparison of Two Mass Spectrometry Methods (LC-MS/HRMS and LC-MS/MS) for the Quantification of Rituximab in Human Plasma

Molecules, 2021
Rituximab is a chimeric immunoglobulin G1-kappa (IgG1κ) antibody targeting the CD20 antigen on B-lymphocytes. Its applications are various, such as for the treatment of chronic lymphoid leukemia or non-Hodgkin’s lymphoma in oncology, and it can also be ...
Aurélien Millet, Nihel Khoudour, Dorothée Lebert, Christelle Machon, Benjamin Terrier, Benoit Blanchet, Jérôme Guitton   +6 more
doaj   +1 more source