Results 111 to 120 of about 111,920 (305)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
, 2012 Includes bibliographical references.The principles of lean thinking have been widely applied but lean implementing is notoriously complex and challenging.
Isaacs, Devin
core
Lean Construction – 2007 to 2016 [PDF]
, 2016 Aiming at identify main keywords of Recent Works published the annals of the International Group for Lean Construction Conference ( IGLC ) so Get a retrospective of the main ITS applications, actions and tools used lean .It was analyzed all articles ...
Monte, Carlos Eduardo +2 more
core
EngMachining Nomex honeycomb structures presents a major challenge due to their thin-walled architecture, orthotropic behavior, and sensitivity to adhesion and delamination.
Oussama Beldi +7 more
doaj +1 more source
Advances in Mechanical Engineering, 2019 This article proposes a new model for concurrent Lean-Kaizen based on the Lean production thinking. According to the concept and application scenarios of value stream mapping and DMAIC (Define, Measure, Analyze, Improve, and Control), we proposed an ...
Wei Guo +3 more
doaj +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Assessment of Lean implementation in Hotels’ supply chains
ProductionPaper aims This article aims at investigating the implementation level of Lean Supply Chain (LSC) practices in hotels’ supply chains. Originality The proposed method allows to characterize LSC implementation in such an unusual context, identifying ...
Guilherme Luz Tortorella +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
MiR‐513a promotes human erythroid differentiation by modulating c‐Jun
FEBS Open Bio, EarlyView.During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim +11 more
wiley +1 more source