Results 1 to 10 of about 212,497 (330)

Sand flies: Basic information on the vectors of leishmaniasis and their interactions with Leishmania parasites

Communications Biology, 2022
Blood-sucking arthropods transmit a variety of human pathogens acting as disseminators of the so-called vector-borne diseases. Leishmaniasis is a spectrum of diseases caused by different Leishmania species, transmitted quasi worldwide by sand flies ...
P. Cecílio, A. Cordeiro-da-Silva, Fabiano Oliveira   +2 more
semanticscholar   +1 more source

Antibiotic-Free Nanoplasmids as Promising Alternatives for Conventional DNA Vectors

Vaccines, 2022
DNA vaccines with their extraordinary properties are the best choice as vectors for subunit vaccines but are not in compliance with safety regulations, mainly because of the antibiotic resistance genes on their backbone.
Negar Seyed, Farnaz Zahedifard, Sima Habibzadeh, Roya Yousefi, Mahya Sadat Lajevardi, Elham Gholami, Sima Rafati   +6 more
doaj   +1 more source

Leishmania tarentolae as Potential Live Vaccine Co-Expressing Distinct Salivary Gland Proteins Against Experimental Cutaneous Leishmaniasis in BALB/c Mice Model

Frontiers in Immunology, 2022
Leishmaniasis is a neglected vector-borne disease caused by Leishmania parasites transmitted through the infected sand flies bite. Current treatments are limited, partly due to their high cost and significant adverse effects, and no human vaccine is yet ...
Mahya Sadat Lajevardi, Mahya Sadat Lajevardi, Elham Gholami, Tahereh Taheri, Hamzeh Sarvnaz, Sima Habibzadeh, Negar Seyed, Yousef Mortazavi, Sima Rafati   +8 more
doaj   +1 more source

Structural analysis of PpSP15 and PsSP9 sand fly salivary proteins designed with a self-cleavable linker as a live vaccine candidate against cutaneous leishmaniasis

Parasites & Vectors, 2022
Background Leishmania parasites are deposited in the host through sand fly bites along with sand fly saliva. Therefore, salivary proteins are promising vaccine candidates for controlling leishmaniasis.
Mahya Sadat Lajevardi, Tahereh Taheri, Elham Gholami, Negar Seyed, Sima Rafati   +4 more
doaj   +1 more source

Promastigote-to-Amastigote Conversion in Leishmania spp.—A Molecular View

Pathogens, 2022
A key factor in the successful infection of a mammalian host by Leishmania parasites is their conversion from extracellular motile promastigotes into intracellular amastigotes. We discuss the physical and chemical triggers that induce this conversion and
Joachim Clos, Janne Grünebast, Myrine Holm   +2 more
doaj   +1 more source

Systematic functional analysis of Leishmania protein kinases identifies regulators of differentiation or survival

Nature Communications, 2020
Differentiation between distinct stages is fundamental for the life cycle of intracellular protozoan parasites and for transmission between hosts, requiring stringent spatial and temporal regulation.
Baker N., Catta-Preta C.M.C., Neish R., Sadlova J., Powell B., Alves-Ferreira E.V.C., Geoghegan V., Carnielli J.B.T., Newling K., Hughes C., Vojtkova B., A. J, Mihut A., Walrad P.B., Wilson L.G., Pitchford J.W., V. P., Mottram J.C.   +17 more
semanticscholar   +1 more source

A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing

Nature Communications, 2020
Leishmaniasis is a neglected tropical disease caused by Leishmania protozoa transmitted by infected sand flies. Vaccination through leishmanization with live Leishmania major has been used successfully but is no longer practiced because it resulted in ...
Wen Wei Zhang, S. Karmakar, Sreenivas Gannavaram, R. Dey, Patrick Lypaczewski, Nevien Ismail, A. Siddiqui, V. Simonyan, Fabiano Oliveira, Iliano V. Coutinho-Abreu, Thiago DeSouza-Vieira, C. Meneses, James Oristian, T. D. Serafim, Abu Musa, R. Nakamura, N. Saljoughian, Greta Volpedo, Monika Satoskar, Monika Satoskar, S. Satoskar, S. Satoskar, Pradeep K. Dagur, J. McCoy, S. Kamhawi, J. Valenzuela, S. Hamano, A. Satoskar, G. Matlashewski, H. Nakhasi   +29 more
semanticscholar   +1 more source

Cultivation of Protozoa Parasites In Vitro: Growth Potential in Conventional Culture Media versus RPMI-PY Medium

Veterinary Sciences, 2023
The in vitro cultivation of Leishmania and Trypanosoma parasites plays an important role in the diagnosis and treatment of parasitic diseases. Although Evans’s modified Tobie and Novy–MacNeal–Nicolle media, for Leishmania spp.
Germano Castelli, Eugenia Oliveri, Viviana Valenza, Susanna Giardina, Flavia Facciponte, Francesco La Russa, Fabrizio Vitale, Federica Bruno   +7 more
doaj   +1 more source

An interactive database of Leishmania species distribution in the Americas

Scientific Data, 2020
The Americas have an elevated number of leishmaniasis cases (accounting for two-thirds of the worldwide disease burden) and circulating Leishmania species, and are therefore of interest in terms of epidemiological surveillance.
Giovanny Herrera, Natalia Barragán, Nicolás Luna, David Martínez, Frasella De Martino, J. Medina, Sergio Niño, Luisa Páez, Angie L Ramírez, Laura Vega, Valeria Velandia, Michelle Vera, María Fernanda Zúñiga, Marius Bottin, J. Ramírez   +14 more
semanticscholar   +1 more source

Molecular Diagnosis of Leishmaniasis: Quantification of Parasite Load by a Real-Time PCR Assay with High Sensitivity

Pathogens, 2021
Real-time PCR was developed to quantify Leishmania infantum kinetoplast DNA and optimized to achieve a sensitivity of 1 parasite/mL. For this purpose, we cloned the conserved kDNA fragment of 120 bp into competent cells and correlated them with serial ...
Germano Castelli, Federica Bruno, Stefano Reale, Simone Catanzaro, Viviana Valenza, Fabrizio Vitale   +5 more
doaj   +1 more source