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Desenvolvimento de um teste colorimétrico para triagem da atividade leishmanicida de compostos utilizando Leishmania amazonensis expressando a enzima beta-galactosidase [PDF]
, 2013 Dissertação (mestrado) - Universidade Federal de Santa Catarina, Programa de Pós-graduação em Biotecnologia e Biociências, Florianópolis, 2013No presente trabalho transfectamos uma cepa de Leishmania amazonensis com um plasmídeo contendo o gene da ...
Tonini, Maiko Luis
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Leishmania major: Parasite Interactions Suggesting Sexuality
Memorias do Instituto Oswaldo Cruz, 1997 In five experiments, Leishmania (Leishmania) major (MRHO/SU/59/P-strain) grew poorly when seeded in FYTS medium supplemented with 15% fetal calf serum, but presented several peculiar pairs of promastigotes diametrically opposed and attached at their ...
Sousa Maria Auxiliadora de +2 more
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The Regulation of Immunity to Leishmania Major
Annual Review of Immunology, 1995Experimental infection with the intracellular protozoan Leishmania major constitutes a particularly versatile model for assessing the role of CD4+ subset development in the host response to infectious disease. The association of Th1 development with control of infection, and of Th2 cell development with progressive disease, has been well established ...
S L, Reiner, R M, Locksley
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Folylpolyglutamates in Leishmania major
Biochemical and Biophysical Research Communications, 1987The intracellular folates of the protozoan parasite Leishmania major have been examined. About 95% of the exogenous [3H]folate accumulated by the protozoan is metabolized to polyglutamate conjugates within 65 hr, and the intracellular folates are about forty-fold concentrated over the folate in the medium.
D V, Santi, P, Nolan, B, Shane
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Trypanothione biosynthesis in Leishmania major
Molecular and Biochemical Parasitology, 2005Trypanothione plays a crucial role in regulation of intracellular thiol redox balance and in defence against chemical and oxidant stress. Crithidia fasciculata requires two enzymes for the formation of trypanothione, namely glutathionylspermidine synthetase (GspS; EC 6.3.1.8) and a glutathionylspermidine-dependent trypanothione synthetase (TryS; EC 6.3.
Oza, Sandra L. +3 more
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Leishmania major infection: the overture
Parasitology Today, 1995Local infection of mice with Leishmania major results in either healing or death depending on the preferential action of Th1 or Th2 T helper cells, respectively. Although the parasite-induced T-cell responses and their consequences for the disease are well understood, relatively little is known about the initial events that kindle the adaptive immune ...
W, Solbach, T, Laskay
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Brazilian Leishmania Stocks Phenotypically Similar to Leishmania Major
The American Journal of Tropical Medicine and Hygiene, 1985Screening by enzyme electrophoresis of isolates of New World Leishmania from different geographic areas revealed a number of stocks with enzyme profiles different from those produced by reference strains of described subspecies of L. mexicana, L. braziliensis, and L. donovani.
H, Momen +5 more
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The emergence of Leishmania major and Leishmania donovani in southern Turkey
Transactions of the Royal Society of Tropical Medicine and Hygiene, 2014In southern Turkey, Leishmania tropica and L. infantum are both the causative agents of cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL), respectively. However, L. major and L. donovani were known to exist after the influx of Syrian refugees.Between the years of July 2003 and July 2013, a total of 167 smears and 113 bone marrow samples were
Koltas I.S. +3 more
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Extracellular phosphorylation in the parasite, Leishmania major
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1990Leishmania major promastigotes were analyzed for the presence of protein phosphatase activity in intact cells and membrane-enriched fractions. Parasite phosphoproteins, phosphorylated in live cells with [gamma-32P]adenosine 5'-triphosphate (ATP) and an endogenous leishmanial ectokinase, were dephosphorylated by endogenous protein phosphatase-like ...
D S, Lester, T, Hermoso, C L, Jaffe
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Leishmania infantum and L. major in Algeria
Transactions of the Royal Society of Tropical Medicine and Hygiene, 1996Since 1980, the development of leishmaniasis in Algeria has been marked by a considerable increase in the number of cases of both visceral leishmaniasis (1121 cases recorded) and cutaneous leishmaniasis (more than 2000 cases per year). New Leishmania infantum and L. major foci have appeared in the north and south of the country. During this period, 100
Z, Harrat +7 more
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