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Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells
2009Human fetal mesenchymal stem cells (hfMSC) demonstrate extensive expansion and differentiation capacities and are hence being studied for use in stem cell therapeutics, including gene delivery. With advanced prenatal diagnosis, fetal gene therapy represents an additional avenue for the treatment of inherited deficiencies.
Mark S K, Chong, Jerry, Chan
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Knocking down schistosomes – promise for lentiviral transduction in parasites
Trends in Parasitology, 2015Underpinned by major advances in our understanding of the genomes of schistosomes, progress in the development of functional genomic tools is providing unique prospects to gain insights into the intricacies of the biology of these blood flukes, their host relationships, and the diseases that they cause.
Jana, Hagen +2 more
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Lentiviral transduction of microglial cells.
Glia, 2005Microglial cells are the resident immune cells of the central nervous system. Their function resembles that of tissue macrophages and, as such, they share many properties with both peripheral macrophages and monocytes. One striking similarity is the difficulty with which these cells can be genetically manipulated via transfection or transduction.
Stephanie, Balcaitis +4 more
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Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction
2015Recombinant lentiviral vectors are powerful tools to stably manipulate human pluripotent stem cells. They can be used to deliver ectopic genes, shRNAs, miRNAs, or any possible genetic DNA sequence into diving and nondividing cells. Here we describe a general protocol for the production of self-inactivating lentiviral vector particles and their ...
Ortwin, Naujok +2 more
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Lentiviral Gene Transduction of Mouse and Human Stem Cells
2008This chapter describes the methods we use to transduce mouse and human hematopoietic stem cells (HSCs) and human embryonic stem cells (hESCs). We provide detailed protocols for producing high-titer lentiviral supernatants by transient transfection and for measuring viral titers.
Zhaohui, Ye +2 more
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Efficient lentiviral transduction of liver requires cell cycling in vivo
Nature Genetics, 2000Human-immunodeficiency-virus (HIV)-based lentiviral vectors are a promising tool for in vivo gene therapy. Unlike Moloney-murine-leukaemia-based retroviruses (MLV), lentiviruses are believed to stably transduce quiescent (non-cycling) cells in various organs.
PARK F +4 more
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Optimizing Lentiviral Transduction of Human Natural Killer Cells
Blood, 2011Abstract Abstract 4714 The development of an efficient method to genetically modify natural killer (NK) cells could be used to characterize NK cell differentiation, acquisition of self-tolerance, tumor trafficking in vivo, as well as to manipulate NK cells to enhance their activity against infectious diseases and tumors ...
Su Su +9 more
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Lentiviral Vector Production, Titration, and Transduction of Primary Neurons
2013Lentiviral vectors have become very useful tools for transgene delivery. Based on their ability to transduce both dividing and nondividing cells and to produce long-term transgene expression, lentiviruses have found numerous applications in the biomedical sciences, including developmental neuroscience.
Baojin, Ding, Daniel L, Kilpatrick
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SYSTEMIC AND CARDIOVASCULAR TRANSDUCTION WITH LENTIVIRAL VECTORS
2001The present invention relates to a method for obtaining systemic delivery and/or stable systemic transduction and/or stable cardiovascular transduction of a lentiviral vector, comprising introduction of said lentiviral vector into an animal.
VLAAMS INTERUNIV INST BIOTECH +3 more
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Lentiviral transduction of microglial cells
Glia, 2004Stephanie Balcaitis +4 more
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