Results 281 to 290 of about 368,855 (303)

Lentiviral transduction of microglial cells.

Glia, 2005
Microglial cells are the resident immune cells of the central nervous system. Their function resembles that of tissue macrophages and, as such, they share many properties with both peripheral macrophages and monocytes. One striking similarity is the difficulty with which these cells can be genetically manipulated via transfection or transduction.
Stephanie, Balcaitis, Jonathan R, Weinstein, Sheng, Li, Jeffrey S, Chamberlain, Thomas, Möller   +4 more
openaire   +1 more source

Lentiviral Vector Production, Titration, and Transduction of Primary Neurons

2013
Lentiviral vectors have become very useful tools for transgene delivery. Based on their ability to transduce both dividing and nondividing cells and to produce long-term transgene expression, lentiviruses have found numerous applications in the biomedical sciences, including developmental neuroscience.
Baojin, Ding, Daniel L, Kilpatrick
openaire   +2 more sources

Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells

2009
Human fetal mesenchymal stem cells (hfMSC) demonstrate extensive expansion and differentiation capacities and are hence being studied for use in stem cell therapeutics, including gene delivery. With advanced prenatal diagnosis, fetal gene therapy represents an additional avenue for the treatment of inherited deficiencies.
Mark S K, Chong, Jerry, Chan
openaire   +2 more sources

Lentiviral Gene Transduction of Mouse and Human Stem Cells

2008
This chapter describes the methods we use to transduce mouse and human hematopoietic stem cells (HSCs) and human embryonic stem cells (hESCs). We provide detailed protocols for producing high-titer lentiviral supernatants by transient transfection and for measuring viral titers.
Zhaohui, Ye, Xiaobing, Yu, Linzhao, Cheng   +2 more
openaire   +2 more sources

Improving Lentiviral Transduction Efficiency with Microfluidic Systems

Blood, 2015
Abstract Background: Recent clinical trials have demonstrated the efficacy and safety of gene therapy utilizing HIV-derived lentiviral vectors (LVs) for blood disorders. However, the LV requirements and clinical ex vivo cell transduction protocols used in these studies exposes the limitations of the technology and beckons the need for ...
Reginald Tran, David R Myers, Jordan E Shields, Byungwook Ahn, Yongzhi Qiu, Caroline Hansen, Yumiko Sakurai, Robert Moot, H. Trent Spencer, Christopher B Doering, Wilbur A Lam   +10 more
openaire   +1 more source

Efficient lentiviral transduction of liver requires cell cycling in vivo

Nature Genetics, 2000
Human-immunodeficiency-virus (HIV)-based lentiviral vectors are a promising tool for in vivo gene therapy. Unlike Moloney-murine-leukaemia-based retroviruses (MLV), lentiviruses are believed to stably transduce quiescent (non-cycling) cells in various organs.
PARK F, OHASHI K, CHIU W, NALDINI , LUIGI, KAY MA   +4 more
openaire   +3 more sources

Magnetically guided lentiviral‐mediated transduction of airway epithelial cells

The Journal of Gene Medicine, 2010
AbstractBackgroundLentiviral (LV) vectors are able to only slowly and inefficiently transduce nondividing cells such as those of the airway epithelium. To address this issue, we have exploited the magnetofection technique in in vitro models of airway epithelium.MethodsMagnetofectins were formed by noncovalent interaction between LV particles and ...
C. ORLANDO C, CASTELLANI, STEFANO, O. MYKHAYLYK, E. COPRENI, O. ZELPHATI, C. PLANK, CONESE, MASSIMO   +6 more
openaire   +2 more sources

Generation of Mouse-Induced Pluripotent Stem Cells by Lentiviral Transduction

2019
Terminally differentiated somatic cells can be reprogrammed into an embryonic stem cell-like state by the forced expression of four transcription factors: Oct4, Klf4, Sox2, and c-Myc (OKSM). These so-called induced pluripotent stem (iPS) cells can give rise to any cell type of the body and thus have tremendous potential for many applications in ...
Liu, Xiaodong, Chen, Joseph, Firas, Jaber, Paynter, Jacob M., Nefzger, Christian M., Polo, Jose M.   +5 more
openaire   +5 more sources

Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets

2003
ABSTARCT: Lentiviral vectors based upon human immunodeficiency type I (HIV) are increasingly being used to transduce nondividing or terminally differentiated cells, despite the fact HIV is a known, lethal pathogen. This is because lentiviruses contain multiple gene products that allow infection of cells independent of mitosis. Typically, plasmid DNA is
openaire   +2 more sources

Enhancing the potency of in vivo lentiviral vector mediated gene therapy to hepatocytes

Nature Communications
In vivo gene therapy to the liver using lentiviral vectors (LV) may represent a one-and-done therapeutic approach for monogenic diseases. Increasing LV gene therapy potency is crucial for reducing the effective doses, thus alleviating dose-dependent ...
Cesare Canepari, Michela Milani, Chiara Simoni, Francesco Starinieri, M. Volpin, Anna Fabiano, Mauro Biffi, Fabio Russo, R. Norata, Martina Rocchi, C. Brombin, Federica Cugnata, E. Montini, F. Sanvito, Markus Grompe, Alessio Cantore   +15 more
semanticscholar   +1 more source