Results 261 to 270 of about 260,635 (281)

Magnetically guided lentiviral‐mediated transduction of airway epithelial cells

The Journal of Gene Medicine, 2010
AbstractBackgroundLentiviral (LV) vectors are able to only slowly and inefficiently transduce nondividing cells such as those of the airway epithelium. To address this issue, we have exploited the magnetofection technique in in vitro models of airway epithelium.MethodsMagnetofectins were formed by noncovalent interaction between LV particles and ...
C. ORLANDO C, CASTELLANI, STEFANO, O. MYKHAYLYK, E. COPRENI, O. ZELPHATI, C. PLANK, CONESE, MASSIMO   +6 more
openaire   +2 more sources

Improving Lentiviral Transduction Efficiency with Microfluidic Systems

Blood, 2015
Abstract Background: Recent clinical trials have demonstrated the efficacy and safety of gene therapy utilizing HIV-derived lentiviral vectors (LVs) for blood disorders. However, the LV requirements and clinical ex vivo cell transduction protocols used in these studies exposes the limitations of the technology and beckons the need for ...
Reginald Tran, David R Myers, Jordan E Shields, Byungwook Ahn, Yongzhi Qiu, Caroline Hansen, Yumiko Sakurai, Robert Moot, H. Trent Spencer, Christopher B Doering, Wilbur A Lam   +10 more
openaire   +1 more source

Generation of Mouse-Induced Pluripotent Stem Cells by Lentiviral Transduction

2019
Terminally differentiated somatic cells can be reprogrammed into an embryonic stem cell-like state by the forced expression of four transcription factors: Oct4, Klf4, Sox2, and c-Myc (OKSM). These so-called induced pluripotent stem (iPS) cells can give rise to any cell type of the body and thus have tremendous potential for many applications in ...
Liu, Xiaodong, Chen, Joseph, Firas, Jaber, Paynter, Jacob M., Nefzger, Christian M., Polo, Jose M.   +5 more
openaire   +5 more sources

Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets

2003
ABSTARCT: Lentiviral vectors based upon human immunodeficiency type I (HIV) are increasingly being used to transduce nondividing or terminally differentiated cells, despite the fact HIV is a known, lethal pathogen. This is because lentiviruses contain multiple gene products that allow infection of cells independent of mitosis. Typically, plasmid DNA is
openaire   +2 more sources

Lentiviral transduction of microglial cells

Glia, 2004
Stephanie Balcaitis, Jonathan R. Weinstein, Sheng Li, Jeffrey S. Chamberlain, Thomas Möller   +4 more
openaire   +1 more source

LENTIVIRAL TRANSDUCTION METHODS

2021
SILK JONATHAN, MCEWEN-SMITH ROSANNA, JAPELJ NIKA, HAMILTON GARTH   +3 more
openaire   +5 more sources

A Straightforward Microfluidic-Based Approach Toward Optimizing Transduction Efficiency of HIV-1-Derived Lentiviral Vectors in BCP-ALL Cells

Biotechnology Reports (Amsterdam, Netherlands), 2023
Seyed Esmaeil Ahmadi, Majid Safa
exaly  

Concise Review on Optimized Methods in Production and Transduction of Lentiviral Vectors in Order to Facilitate Immunotherapy and Gene Therapy

Biomedicine and Pharmacotherapy, 2020
Ali Jafari, Ruhollah Dorostkar, Hadi Esmaeili Gouvarchin Ghaleh   +2 more
exaly  

Prostaglandin E2 Increases Lentiviral Vector Transduction Efficiency of Adult Human Hematopoietic Stem and Progenitor Cells

Molecular Therapy, 2018
Bruce E Torbett, Francis J Pierciey, Gábor Veres   +2 more
exaly  

Critical Factors Influencing Stable Transduction of Human CD34+ Cells With HIV-1-Derived Lentiviral Vectors

Molecular Therapy, 2000
Gay M Crooks, Donald B. Kohn, Dennis L Haas   +2 more
exaly