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Lentiviral Transduction of Immune Cells [PDF]
, 2008 Gene transfer into mammalian cells has been of crucial importance for studies determining the role of specific genes in the differentiation and cell fate of various hematopoietic lineages. Until recently, the majority of these studies were performed in transformed cell lines due to difficulties in achieving levels of transfection of greater than 1-3 ...
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Lentiviral Transduction of Primary Human Glioblastoma Cultures.
Methods in molecular biology, 2018This chapter provides detailed step-by-step instructions for the production of lentiviral particles and the transduction of primary human glioblastoma cultures. Lentiviruses stably transduce both dividing and non-dividing cells, such as quiescent cancer stem cell populations.
J. Frenster +2 more
semanticscholar +3 more sources
Improving Lentiviral Transduction of CD34 + Hematopoietic Stem and Progenitor Cells
Human Gene Therapy Methods, 2018Nicolaus Kröger +2 more
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Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4 [PDF]
, 2019 Lentiviral systems have proven advantageous in the delivery and long-term integration of gene sequences into the genome of several cell types in vitro, in vivo, as well as in clinical trials. Here we detail the protocols involved in the molecular cloning of ADAMTSL2 and ADAMTSL4 into the human immunodeficiency virus (HIV)-derived pCDH lentiviral system.
Singh, Mukti +2 more
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Poloxamer synperonic F108 improves cellular transduction with lentiviral vectors [PDF]
Journal of Gene Medicine, 2012 Although lentiviral transduction methods are widely used, their broader application is dependent upon the optimization of lentiviral transduction efficiency for a broad range of cell types. In the present study, we focus on the evaluation of two chemical
Inés Höfig +2 more
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Lentiviral Gene Transduction of Kidney
Human Gene Therapy, 2002Gene transfer into kidney holds great potential as a novel therapeutic approach. We have studied the transduction of kidney in vivo after delivery of lentiviral vectors by various routes of administration. A lentiviral vector expressing the bacterial lacZ gene from the cytomegalovirus early promoter was used.
G Luca, Gusella +5 more
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Optimized Lentiviral Transduction Protocols by Use of a Poloxamer Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G. [PDF]
Methods in molecular biology, 2016 Lentiviral vectors (LV) are widely used to successfully transduce cells for research and clinical applications. This optimized LV infection protocol includes a nontoxic poloxamer-based adjuvant combined with antibody-retargeted lentiviral particles.
Nataša Anastasov +4 more
semanticscholar +4 more sources
Transduction of human islets with the lentiviral vector
Transplantation Proceedings, 2004Establishment of an efficient gene delivery system for human pancreatic beta cells is important for the development of diabetes-targeted cell therapies. The human immunodeficiency virus type 1 (HIV-1)-derived lentiviral vector is well documented to be an effective gene transfer tool for various types of cells.
N, Kobayashi +8 more
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Lentiviral Transduction of Cultured Microglia
2013Microglial cells are the resident immune-related glial cells of the central nervous system (CNS) that are crucial for maintaining homeostasis and sensing pathological alterations in the nervous system. To improve our understanding of the biological function of microglia, gene-transfer techniques have been improved and become widely used over the past ...
Takahiro, Masuda +3 more
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