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Lentiviral Transduction of Mammary Epithelial Cells
2022Lentiviral vectors are the workhorses of modern cell biology. They can infect a wide variety of cells including non-dividing cells and stem cells. They integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100% of the cells in a culture and possible to infect cells simultaneously with multiple vectors ...
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Lentiviral Transduction of Immune Cells
2008Gene transfer into mammalian cells has been of crucial importance for studies determining the role of specific genes in the differentiation and cell fate of various hematopoietic lineages. Until recently, the majority of these studies were performed in transformed cell lines due to difficulties in achieving levels of transfection of greater than 1-3 ...
Swainson, Louise +4 more
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Lentiviral transduction of murine oligodendrocytes in vivo
Journal of Neuroscience Research, 2005Lentiviral vectors are used widely to direct efficient gene transfer in vivo. We examined cell-specific expression in adult murine white matter after stereotaxic microinjection of four lentiviral constructs. We synthesized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviruses with combinations of two promoters, cytomegalovirus (CMV ...
Sally R, McIver +6 more
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Retroviral/Lentiviral Transduction and Transformation Assay
2009Non-random chromosomal translocations can be found in about half of acute leukaemia patients and mostly lead to either over-expression of proto-oncogenes or creation of novel fusion genes. To assess the oncogenic potential and characterize the underlying mechanisms mediated by these candidate oncoproteins, a retroviral transduction/transformation assay
Bernd B, Zeisig, Chi Wai Eric, So
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Transduction of Human Islets with Pseudotyped Lentiviral Vectors
Human Gene Therapy, 2004Type I diabetes is caused by an autoimmune-mediated elimination of insulin-secreting pancreatic islets. Genetic modification of islets offers a powerful molecular tool for improving our understanding of islet biology. Moreover, efficient genetic engineering of islets could allow for evaluation of new strategies aimed at preventing islet destruction ...
Gary P, Kobinger +7 more
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Lentiviral Transduction of Mammary Epithelial Cells
2015Lentiviral vectors are the workhorses of modern cell biology. They can infect a wide variety of cells including nondividing cells and stem cells. They integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100 % of the cells in a culture and possible to infect cells simultaneously with multiple vectors ...
Richard, Iggo, Elodie, Richard
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Stable Expression by Lentiviral Transduction of Cells
2018Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary cells including "hard-to-transfect" cells. As a consequence of the integration of the recombinant lentiviral vector into the cellular genome, the transgene is stably maintained, and long-term producing cells are established. Here,
Natascha, Gödecke +2 more
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Lentiviral Transduction of Human Dendritic Cells
2004Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that play a pivotal role in stimulating antigen-specific T cells in vivo. The cardinal properties of DCs are: the ability to take up, process, and present antigens; (2) the ability to migrate through different tissues into lymphoid organs; and (3) the ability to interact with and ...
Roland, Schroers, Si-Yi, Chen
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Generation of CAR-T Cells by Lentiviral Transduction
2021CAR-T cell therapy is one of the most successful cell-based therapies. T cells are the most common cells to be genetically modified for cancer therapy, not only because T cells have cytotoxicity but also because they are easily cultured ex vivo and genetically modified with viral vectors.
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Knocking down schistosomes – promise for lentiviral transduction in parasites
Trends in Parasitology, 2015Underpinned by major advances in our understanding of the genomes of schistosomes, progress in the development of functional genomic tools is providing unique prospects to gain insights into the intricacies of the biology of these blood flukes, their host relationships, and the diseases that they cause.
Jana, Hagen +2 more
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