Results 231 to 240 of about 260,635 (281)

Microfluidic-based rapid generation of chimeric antigen receptor T cells improves low-multiplicity-of-infection transduction efficiency while preserving functional activity. [PDF]

J Transl Med
Jiang Y, Zhu G, Zhang H, Tang D.
europepmc   +1 more source

Minimal CBX3-Derived UCOE Confers Long-Term Resistance to Transgene Silencing in Human iPSCs During Neuronal Differentiation. [PDF]

Int J Mol Sci
Anakok OF, Akcay G.
europepmc   +1 more source

Macrophage Mistargeting During Direct Cardiac Reprogramming: Cardiac-Associated Responses, Polarization, and Cell Death. [PDF]

Biomedicines
Chen T, Ding S, Zhang Z, Zhao L, Cao K, Zhang G, Weng C, Gong G, Xu L, Yang M, Li T, Sheng C, Li Y.   +12 more
europepmc   +1 more source

Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery. [PDF]

Curr Protoc
Benedetti L, Yi HA, Wang L, Lippincott-Schwartz J.   +3 more
europepmc   +1 more source

[Transduction of hematopoietic stem cells by lentiviral vectors].

Uirusu, 2003
openaire   +1 more source

Poloxamer Synperonic F108 Improves Cellular Transduction With Lentiviral Vectors

Journal of Gene Medicine, 2012
Although lentiviral transduction methods are widely used, their broader application is dependent upon the optimization of lentiviral transduction efficiency for a broad range of cell types. In the present study, we focus on the evaluation of two chemical
Ines Höfig, Natasa Anastasov, Christian Thirion   +2 more
exaly   +3 more sources

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Lentiviral Transduction of Neuronal Cells

2021
Lentiviruses are a very reliable class of viral vectors wildly used in gene therapy. In this chapter, we described a general method for the construction of lentiviral delivery system by using a derived HIV-1 based lentivirus expression vector pKLV-Puro containing a monomeric blue fluorescent protein mammalian codon-optimized (TagBFP).
Anna, Bellizzi, Nicholas, Ahye, Hassen S, Wollebo   +2 more
openaire   +2 more sources

Lentiviral Gene Transduction of Kidney

Human Gene Therapy, 2002
Gene transfer into kidney holds great potential as a novel therapeutic approach. We have studied the transduction of kidney in vivo after delivery of lentiviral vectors by various routes of administration. A lentiviral vector expressing the bacterial lacZ gene from the cytomegalovirus early promoter was used.
G Luca, Gusella, Elena, Fedorova, Basil, Hanss, Daniele, Marras, Mary E, Klotman, Paul E, Klotman   +5 more
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Transduction of human islets with the lentiviral vector

Transplantation Proceedings, 2004
Establishment of an efficient gene delivery system for human pancreatic beta cells is important for the development of diabetes-targeted cell therapies. The human immunodeficiency virus type 1 (HIV-1)-derived lentiviral vector is well documented to be an effective gene transfer tool for various types of cells.
N, Kobayashi, T, Arata, T, Okitsu, H, Ikeda, K, Kobayashi, Y, Kosaka, M, Narushima, N, Tanaka, J R T, Lakey   +8 more
openaire   +2 more sources

Lentiviral Transduction of Cultured Microglia

2013
Microglial cells are the resident immune-related glial cells of the central nervous system (CNS) that are crucial for maintaining homeostasis and sensing pathological alterations in the nervous system. To improve our understanding of the biological function of microglia, gene-transfer techniques have been improved and become widely used over the past ...
Takahiro, Masuda, Makoto, Tsuda, Hidetoshi, Tozaki-Saitoh, Kazuhide, Inoue   +3 more
openaire   +2 more sources