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Lentiviral Vector Bioprocessing [PDF]
Viruses, 2021 Lentiviral vectors (LVs) are potent tools for the delivery of genes of interest into mammalian cells and are now commonly utilised within the growing field of cell and gene therapy for the treatment of monogenic diseases and adoptive therapies such as ...
Christopher Perry, Andrea C. M. E. Rayat
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Analysis of Hepatic Lentiviral Vector Transduction: Implications for Preclinical Studies and Clinical Gene Therapy Protocols [PDF]
VirusesLentiviral vector-transduced T cells were approved by the FDA as gene therapy anti-cancer medications. Little is known about the effects of host genetic variation on the safety and efficacy of the lentiviral vector gene delivery system.
Peirong Hu +5 more
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A targeting lentiviral vector for generation of CAR-T cells in vivo [PDF]
Scientific ReportsChimeric antigen receptor (CAR) T cell therapy has demonstrated remarkable therapeutic efficacy in treating cancer and autoimmune diseases. However, current CAR-T cell therapy requires ex vivo T cell engineering, which is both time-consuming and cost ...
Muhadasi Tuerxunyiming +5 more
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HIV-based lentiviral vectors: Origin and sequence differences
Molecular Therapy: Methods & Clinical Development, 2021 Three gene therapy strategies have received US Food and Drug Administration (FDA) approval; one includes HIV-1-based lentiviral vectors. These vectors incorporate features to provide long-term gene transfer and expression while minimizing generation of a
Nathan M. Johnson +5 more
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Development and validation of a droplet digital PCR method for quantifying lentiviral vector infectious titer [PDF]
HeliyonLentiviruses, with their high transduction efficiency and gene expression levels, are widely used as gene delivery vectors in the development of chimeric antigen receptor T cells (CAR-T) and other genetically modified cell therapies.
Xueling Wu +7 more
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Integrase deficient lentiviral vector: prospects for safe clinical applications [PDF]
PeerJ, 2022 HIV-1 derived lentiviral vector is an efficient transporter for delivering desired genetic materials into the targeted cells among many viral vectors. Genetic material transduced by lentiviral vector is integrated into the cell genome to introduce new ...
Chee-Hong Takahiro Yew +6 more
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The very stable lentiviral vector [PDF]
Molecular Therapy: Methods & Clinical DevelopmentGwendolyn K. Binder, Chien-Chung Chen
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Frontiers in Bioengineering and Biotechnology, 2022 The field of lentiviral vector (LV) production continues to face challenges in large-scale manufacturing, specifically regarding producing enough vectors to meet the demand for treating patients as well as producing high and consistent quality of vectors
Michelle Yen Tran, Amine A. Kamen
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JCI Insight, 2022 Lentiviral vector–based dendritic cell vaccines induce protective T cell responses against viral infection and cancer in animal models. In this study, we tested whether preventative and therapeutic vaccination could be achieved by direct injection of ...
Takuya Tada +3 more
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Process intensification for lentiviral vector manufacturing using tangential flow depth filtration
Molecular Therapy: Methods & Clinical Development, 2023 For gene therapies to become more accessible and affordable treatment options, process intensification is one possible strategy to increase the number of doses generated per batch of viral vector.
Robert M. Tona +6 more
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