Results 21 to 30 of about 1,111,723 (286)

Construction of a single lentiviral vector containing tetracycline-inducible Alb-uPA for transduction of uPA expression in murine hepatocytes. [PDF]

PLoS ONE, 2013
The SCID-beige/Alb-uPA mouse model is currently the best small animal model available for viral hepatitis infection studies [1]. But the construction procedure is often costly and time-consuming due to logistic and technical difficulties.
Jiasi Bai, Jungang Li, Qing Mao
doaj   +1 more source

Rev-dependent lentiviral expression vector

Retrovirology, 2007
Background HIV-responsive expression vectors are all based on the HIV promoter, the long terminal repeat (LTR). While responsive to an early HIV protein, Tat, the LTR is also responsive to cellular activation states and to the local chromatin activity ...
Beddall Margaret H, Wu Yuntao, Marsh Jon W   +2 more
doaj   +1 more source

Integrase-deficient lentiviral vectors mediate efficient gene transfer to human vascular smooth muscle cells with minimal genotoxic risk [PDF]

, 2012
We have previously shown that injury-induced neointima formation was rescued by adenoviral-Nogo-B gene delivery. Integrase-competent lentiviral vectors (ICLV) are efficient at gene delivery to vascular cells but present a risk of insertional mutagenesis.
Sessa, W.C., Chick, H.E., Kane, N.M., Baker, A.H., Thrasher, A.J., Alba, R., Fronza, R., McDonald, R.A., Delles, C., Schmidt, M., Nowrouzi, A.   +10 more
core   +1 more source

Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates

Molecular Therapy: Methods & Clinical Development, 2016
Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in ...
Michele E Murphy, Chintan D Vin, Megan M Slough, Wayne R Gombotz, Brenna Kelley-Clarke   +4 more
doaj   +1 more source

Antisense transcription from lentiviral gene targeting linked to an integrated stress response in colorectal cancer cells

Molecular Therapy: Nucleic Acids, 2022
Advances in gene therapy research have resulted in the successful development of new therapies for clinical use. Here, we explored a gene targeting approach to deplete ephrinB2 from colorectal cancer cells using an inducible lentiviral vector.
Taekyu Ha, Michael DiPrima, Vishal Koparde, Parthav Jailwala, Hidetaka Ohnuki, Jing-Xin Feng, Murali Palangat, Daniel Larson, Giovanna Tosato   +8 more
doaj   +1 more source

Biosafety Features of Lentiviral Vectors [PDF]

Human Gene Therapy, 2013
Over the past decades, lentiviral vectors have evolved as a benchmark tool for stable gene transfer into cells with a high replicative potential. Their relatively flexible genome and ability to transduce many forms of nondividing cells, combined with the potential for cell-specific pseudotyping, provides a rich resource for numerous applications in ...
Schambach A, Zychlinski D, Ehrnstroem B, Baum C.   +3 more
openaire   +2 more sources

Remission of invasive, cancer stem-like glioblastoma xenografts using lentiviral vector-mediated suicide gene therapy [PDF]

, 2009
Background: Glioblastoma is the most frequent and most malignant primary brain tumor with a poor prognosis. The translation of therapeutic strategies for glioblastoma from the experimental phase into the clinic has been limited by insufficient animal ...
Oleg Tsinkalovsky, Euskirchen Philipp, Tsanan Giroglou, Huszthy, Peter C., Giroglou Tsanan, Skaftnesmo, Kai Ove, Miletic, Hrvoje, Miletic Hrvoje, Peter C Huszthy, Kai Ove Skaftnesmo, Tsinkalovsky Oleg, von Laer Dorothee, Euskirchen, Philipp, Dorothee von Laer, Hrvoje Miletic, Laer, Dorothee von, Rolf Bjerkvig, Giroglou, Tsanan, Tsinkalovsky, Oleg, Huszthy Peter C., Skaftnesmo Kai Ove, Bjerkvig Rolf, Philipp Euskirchen, Bjerkvig, Rolf   +23 more
core   +2 more sources

Robust Enhancement of Lentivirus Production by Promoter Activation

Scientific Reports, 2018
Lentiviral vectors are a valuable tool to deliver exogenous genes for stable expression in cells. While much progress has been made in processing lentiviral vector-containing culture medium, it remains to be explored how the production of lentiviral ...
Naoto Suzuki, Takeshi Yoshida, Hiroaki Takeuchi, Ryuta Sakuma, Sayaka Sukegawa, Shoji Yamaoka   +5 more
doaj   +1 more source

Tightly regulated and homogeneous transgene expression in human adipose-derived mesenchymal stem cells by lentivirus with tet-off system. [PDF]

PLoS ONE, 2013
Genetic modification of human adipose tissue-derived multilineage progenitor cells (hADMPCs) is highly valuable for their exploitation in therapeutic applications. Here, we have developed a novel single tet-off lentiviral vector platform.
Hiroyuki Moriyama, Mariko Moriyama, Kei Sawaragi, Hanayuki Okura, Akihiro Ichinose, Akifumi Matsuyama, Takao Hayakawa   +6 more
doaj   +1 more source

The HIV-1 Rev protein enhances encapsidation of unspliced and spliced, RRE-containing lentiviral vector RNA. [PDF]

PLoS ONE, 2012
BACKGROUND: During the RNA encapsidation process of human immunodeficiency virus (HIV) viral genomic, unspliced RNA (gRNA) is preferentially incorporated into assembling virions. However, a certain amount of spliced viral transcripts can also be detected
Bastian Grewe, Katrin Ehrhardt, Bianca Hoffmann, Maik Blissenbach, Sabine Brandt, Klaus Uberla   +5 more
doaj   +1 more source