Results 31 to 40 of about 1,111,723 (286)

Lentiviral Vectors for Ocular Gene Therapy

Pharmaceutics, 2022
This review offers the basics of lentiviral vector technologies, their advantages and pitfalls, and an overview of their use in the field of ophthalmology.
Yvan Arsenijevic, Adeline Berger, Florian Udry, Corinne Kostic   +3 more
doaj   +1 more source

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors [PDF]

, 2004
BACKGROUND: Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the
Hermens, Wim T J M C, Lieberman, A., Eggers, R., Verhaagen, J., Sablitzky, Fred, Chakravarthy, Sridhara, Zhang, Jing Ying, Zhang, J.Y., Hermens, Wim TJMC, Zhang, Jing, Verhaagen, J, Ahmed, Bushra, Anderson, P.N., Eggers, R, Eggers, Ruben, Levelt, Christiaan, Chakravarthy, S, Levelt, C., Ahmed, BY, Verhaagen, Joost, Lieberman, A.R., Hermens, WTJMC, Lieberman, AR, Anderson, Patrick N., Ahmed, Bushra Y, Hermens, W.T., Niclou, SP, Anderson, PN, NICLOU, Simone P., Lieberman, A R, Zhang, JY, Anderson, Patrick N, Sablitzky, F., Levelt, C, Sablitzky, F, Niclou, S.P., Chakravarthy, S., Ahmed, B.Y.   +37 more
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Protection of stem cell-derived lymphocytes in a primate AIDS gene therapy model after in vivo selection [PDF]

, 2009
Background: There is currently no effective AIDS vaccine, emphasizing the importance of developing alternative therapies. Recently, a patient was successfully transplanted with allogeneic, naturally resistant CCR5-negative (CCR5 delta 32) cells, setting ...
Trobridge, Grant D., Chiu Sum Ying, Brian C Beard, Robert A Wu, John J Rossi, Muñoz Nina M., Nina M Muñoz, John J. Rossi, Brian C. Beard, Dorothee von Laer, Laer, Dorothee von, Grant D Trobridge, Beard Brian C., Chiu, Sum Ying, Nina M. Muñoz, Kiem, Hans-Peter, Sum Ying Chiu, Trobridge Grant D., Wu, Robert A., Hans-Peter Kiem, Kiem Hans-Peter, Beard, Brian C., Wu Robert A., von Laer Dorothee, Rossi, John J., Rossi John J., Grant D. Trobridge, Robert A. Wu, Muñoz, Nina M.   +28 more
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Minimal impact of ZAP on lentiviral vector production and transduction efficiency

Molecular Therapy: Methods & Clinical Development, 2021
The antiviral protein ZAP binds CpG dinucleotides in viral RNA to inhibit replication. This has likely led to the CpG suppression observed in many RNA viruses, including retroviruses.
Helin Sertkaya, Laura Hidalgo, Mattia Ficarelli, Dorota Kmiec, Adrian W. Signell, Sadfer Ali, Hannah Parker, Harry Wilson, Stuart J.D. Neil, Michael H. Malim, Conrad A. Vink, Chad M. Swanson   +11 more
doaj   +1 more source

Construction of LILRA2-overexpressing lentiviral vector and establishment of THP-1-LILRA2 stable transfected cell strain [PDF]

Jichu yixue yu linchuang
Objective To construct a recombinant lentiviral vector overexpressing leukocyte immunoglobulin-like receptor subfamily A member 2 (LILRA2), establish a stable LILRA2-overexpressing human myeloid leukemia mononuclear cell strain(THP-1), and preliminarily ...
HUANG Gaoxiang, WEI Dongmei, YANG Yao, GUO Xianing, ZHOU Kai, LUAN Jing
doaj   +1 more source

Hybrid Lentiviral Vectors

Molecular Therapy, 2010
Lentiviral vectors have remarkable cell entry and gene delivery properties that make them highly attractive for gene therapy. However, all integration-competent gene delivery systems have come under scrutiny for possible adverse insertional events. Circumventing the risk of insertional mutagenesis, integration-deficient human immunodeficiency virus ...
Qasim, Waseem, Vink, Conrad A, Thrasher, Adrian J   +2 more
openaire   +2 more sources

Dendritic cell–targeted lentiviral vector immunization uses pseudotransduction and DNA-mediated STING and cGAS activation [PDF]

, 2017
Lentiviral vector preparations stimulate immune responses via the host STING and cGAS pathway and pseudotransduction.
Lili Yang, Yong Ouyang, Dai, Bingbing, Kevin K. Lee, Pin Wang, Kim, Jocelyn T, Baltimore, David, Rajan P. Kulkarni, Kulkarni, Rajan P, Jocelyn T. Kim, Ouyang, Yong, Bingbing Dai, Geoffrey Lovely, Yarong Liu, David Baltimore, Liu, Yarong, Yang, Lili, Lee, Kevin K, Lovely, Geoffrey, Wang, Pin   +19 more
core   +1 more source

Delivering genes with human immunodeficiency virus-derived vehicles: still state-of-the-art after 25 years

Journal of Biomedical Science, 2022
Viruses are naturally endowed with the capacity to transfer genetic material between cells. Following early skepticism, engineered viruses have been used to transfer genetic information into thousands of patients, and genetic therapies are currently ...
Jonas Holst Wolff, Jacob Giehm Mikkelsen
doaj   +1 more source

Production of lentiviral vectors

Molecular Therapy - Methods & Clinical Development, 2016
Lentiviral vectors (LV) have seen considerably increase in use as gene therapy vectors for the treatment of acquired and inherited diseases. This review presents the state of the art of the production of these vectors with particular emphasis on their large-scale production for clinical purposes.
Merten, O. W., Hebben, M., Bovolenta, C.
openaire   +3 more sources

Map of lentiviral vector.

, 2012
Map of lentiviral vector.
Yongliang Zhang (122202), Qianyun Xi (171989), Hongyi Li (172014), Ping Gao (172038), Zhenfang Wu (152600), Jinghua Ding (171994), Xiaotong Zhu (172033), Songbo Wang (172029), Junyun Zhou (172009), Qingyan Jiang (172019), Fanmin Meng (172004), Weiguang Cai (172001), Gang Shu (172025)   +12 more
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