Results 251 to 260 of about 260,635 (281)

Lentiviral vector-mediated transduction of goat undifferentiated spermatogonia

Animal Reproduction Science, 2015
Recent studies show that spermatogonial stem cells (SSCs) are able to colonize and form mature spermatozoa following transplantation into germ cell depleted testes of recipient males. Therefore, efficient ways for enrichment and gene transfer into SSCs provides a powerful tool for production of transgenic animals.
Hassan, Abbasi, Sayyed Morteza, Hosseini, Mahdi, Hajian, Zahra, Nasiri, Mehrnoosh, Bahadorani, Mojtaba, Tahmoorespur, Mohammad Reza, Nasiri, Mohammad Hossein, Nasr-Esfahani   +7 more
openaire   +2 more sources

Lentiviral Transduction of Primary Human Glioblastoma Cultures

2018
This chapter provides detailed step-by-step instructions for the production of lentiviral particles and the transduction of primary human glioblastoma cultures. Lentiviruses stably transduce both dividing and non-dividing cells, such as quiescent cancer stem cell populations.
Joshua D, Frenster, Julio, Inocencio, Dimitris G, Placantonakis   +2 more
openaire   +2 more sources

Lentiviral Transduction for Optimal LSC/HSC Manipulation

2020
Historically, efficient transduction of hematopoietic stem cells (HSC) to study the role of specific genes on HSC function, as well as to broaden the potential of gene therapy for hematopoietic related diseases has relied on our ability to design vectors capable of delivering the gene of interest without affecting HSC function.
openaire   +2 more sources

Challenges in Lentiviral Vector Production: Retro-Transduction of Producer Cell Lines [PDF]

Frontiers in Bioengineering and Biotechnology
The increasing demand for lentiviral vectors (LVs) has led to the development of several stable cell lines and production methods over the last 2 decades in order to increase titers and yields, reduce production costs and improve the safety of the vector
Maximilian Klimpel
exaly   +2 more sources

Lentiviral Transduction of Face and Limb Flaps

Plastic and Reconstructive Surgery, 2012
Ex vivo introduction of an immunomodulatory transgene into a face or hand allograft may improve the risk-to-benefit ratio of vascularized composite allografts. Abrogation of the immunogenicity of the skin component of a face or hand allograft may decrease alloreactivity and permit the induction of immunologic tolerance.
Angelo A, Leto Barone, Zhao Y, Zhou, Michael W, Hughes, Ryan, Park, Ruth M, Schulman, Steven, Lee, Evan N, Vidar, Travis L, Shiba, Erin L, Weber, Curtis L, Cetrulo   +9 more
openaire   +2 more sources

Lentiviral transduction of microglial cells.

Glia, 2005
Microglial cells are the resident immune cells of the central nervous system. Their function resembles that of tissue macrophages and, as such, they share many properties with both peripheral macrophages and monocytes. One striking similarity is the difficulty with which these cells can be genetically manipulated via transfection or transduction.
Stephanie, Balcaitis, Jonathan R, Weinstein, Sheng, Li, Jeffrey S, Chamberlain, Thomas, Möller   +4 more
openaire   +1 more source

Lentiviral Vector Production, Titration, and Transduction of Primary Neurons

2013
Lentiviral vectors have become very useful tools for transgene delivery. Based on their ability to transduce both dividing and nondividing cells and to produce long-term transgene expression, lentiviruses have found numerous applications in the biomedical sciences, including developmental neuroscience.
Baojin, Ding, Daniel L, Kilpatrick
openaire   +2 more sources

Lentiviral Gene Transduction of Mouse and Human Stem Cells

2008
This chapter describes the methods we use to transduce mouse and human hematopoietic stem cells (HSCs) and human embryonic stem cells (hESCs). We provide detailed protocols for producing high-titer lentiviral supernatants by transient transfection and for measuring viral titers.
Zhaohui, Ye, Xiaobing, Yu, Linzhao, Cheng   +2 more
openaire   +2 more sources

Efficient lentiviral transduction of liver requires cell cycling in vivo

Nature Genetics, 2000
Human-immunodeficiency-virus (HIV)-based lentiviral vectors are a promising tool for in vivo gene therapy. Unlike Moloney-murine-leukaemia-based retroviruses (MLV), lentiviruses are believed to stably transduce quiescent (non-cycling) cells in various organs.
PARK F, OHASHI K, CHIU W, NALDINI , LUIGI, KAY MA   +4 more
openaire   +3 more sources

Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells

2009
Human fetal mesenchymal stem cells (hfMSC) demonstrate extensive expansion and differentiation capacities and are hence being studied for use in stem cell therapeutics, including gene delivery. With advanced prenatal diagnosis, fetal gene therapy represents an additional avenue for the treatment of inherited deficiencies.
Mark S K, Chong, Jerry, Chan
openaire   +2 more sources