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BaEV-pseudotyped lentiviral vectors enable stable CAR expression and cytotoxic function in NK cells. [PDF]
Park M +8 more
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Lentiviral-mediated gene complementation to rescue pathogenic ABCA3 variants. [PDF]
Cooney AL +12 more
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Efficient NK cell transduction with VSV-G-pseudotyped lentiviral vectors. [PDF]
Järvelä E +18 more
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Safe and Localized Lentiviral Gene Delivery via Injectable Mesoporous Scaffolds for Potent Antitumor Immunity. [PDF]
Nguyen TL +12 more
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[Transduction of hematopoietic stem cells by lentiviral vectors].
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Lentiviral Transduction of Neuronal Cells.
Methods in molecular biology, 2021Lentiviruses are a very reliable class of viral vectors wildly used in gene therapy. In this chapter, we described a general method for the construction of lentiviral delivery system by using a derived HIV-1 based lentivirus expression vector pKLV-Puro containing a monomeric blue fluorescent protein mammalian codon-optimized (TagBFP).
A. Bellizzi +2 more
semanticscholar +3 more sources
Lentiviral Transduction of Mammary Epithelial Cells.
Methods in molecular biology, 2022Lentiviral vectors are the workhorses of modern cell biology. They can infect a wide variety of cells including non-dividing cells and stem cells. They integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100% of the cells in a culture and possible to infect cells simultaneously with multiple vectors ...
R. Iggo
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Generation of CAR-T Cells by Lentiviral Transduction.
Methods in molecular biology, 2021CAR-T cell therapy is one of the most successful cell-based therapies. T cells are the most common cells to be genetically modified for cancer therapy, not only because T cells have cytotoxicity but also because they are easily cultured ex vivo and genetically modified with viral vectors.
Atsushi Okuma
semanticscholar +3 more sources
Stable Expression by Lentiviral Transduction of Cells.
Methods in molecular biology, 2018Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary cells including "hard-to-transfect" cells. As a consequence of the integration of the recombinant lentiviral vector into the cellular genome, the transgene is stably maintained, and long-term producing cells are established. Here,
Natascha Gödecke, H. Hauser, D. Wirth
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