Results 271 to 280 of about 368,855 (303)

Lentiviral transduction of murine oligodendrocytes in vivo

Journal of Neuroscience Research, 2005
Lentiviral vectors are used widely to direct efficient gene transfer in vivo. We examined cell-specific expression in adult murine white matter after stereotaxic microinjection of four lentiviral constructs. We synthesized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviruses with combinations of two promoters, cytomegalovirus (CMV ...
Sally R, McIver, Chul-Sang, Lee, Jin-Moo, Lee, Steven H, Green, Mark S, Sands, B Joy, Snider, Mark P, Goldberg   +6 more
openaire   +2 more sources

Transduction of Human Islets with Pseudotyped Lentiviral Vectors

Human Gene Therapy, 2004
Type I diabetes is caused by an autoimmune-mediated elimination of insulin-secreting pancreatic islets. Genetic modification of islets offers a powerful molecular tool for improving our understanding of islet biology. Moreover, efficient genetic engineering of islets could allow for evaluation of new strategies aimed at preventing islet destruction ...
Gary P, Kobinger, Shaoping, Deng, Jean-Pierre, Louboutin, Marko, Vatamaniuk, Franz, Matschinsky, James F, Markmann, Steven E, Raper, James M, Wilson   +7 more
openaire   +2 more sources

Retroviral/Lentiviral Transduction and Transformation Assay

2009
Non-random chromosomal translocations can be found in about half of acute leukaemia patients and mostly lead to either over-expression of proto-oncogenes or creation of novel fusion genes. To assess the oncogenic potential and characterize the underlying mechanisms mediated by these candidate oncoproteins, a retroviral transduction/transformation assay
Bernd B, Zeisig, Chi Wai Eric, So
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Lentiviral Transduction of Mammary Epithelial Cells

2015
Lentiviral vectors are the workhorses of modern cell biology. They can infect a wide variety of cells including nondividing cells and stem cells. They integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100 % of the cells in a culture and possible to infect cells simultaneously with multiple vectors ...
Richard, Iggo, Elodie, Richard
openaire   +2 more sources

Enhancing lentiviral transduction to generate melanoma-specific human T cells for cancer immunotherapy.

JIM - Journal of Immunological Methods, 2019
Introduction of a tumor antigen-specific T cell receptor (TCR) into patient-derived lymphocytes has already exhibited promising results for the treatment of melanoma and other malignancies in clinical trials. However, insufficient or unsuccessful ex vivo
Bianca Simon, D. C. Harrer, C. Thirion, B. Schuler-Thurner, G. Schuler, U. Uslu   +5 more
semanticscholar   +1 more source

Lentiviral Transduction of Human Dendritic Cells

2004
Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that play a pivotal role in stimulating antigen-specific T cells in vivo. The cardinal properties of DCs are: the ability to take up, process, and present antigens; (2) the ability to migrate through different tissues into lymphoid organs; and (3) the ability to interact with and ...
Roland, Schroers, Si-Yi, Chen
openaire   +2 more sources

Knocking down schistosomes – promise for lentiviral transduction in parasites

Trends in Parasitology, 2015
Underpinned by major advances in our understanding of the genomes of schistosomes, progress in the development of functional genomic tools is providing unique prospects to gain insights into the intricacies of the biology of these blood flukes, their host relationships, and the diseases that they cause.
Jana, Hagen, Jean-Pierre Y, Scheerlinck, Robin B, Gasser   +2 more
openaire   +2 more sources

Lentiviral vector-mediated transduction of goat undifferentiated spermatogonia

Animal Reproduction Science, 2015
Recent studies show that spermatogonial stem cells (SSCs) are able to colonize and form mature spermatozoa following transplantation into germ cell depleted testes of recipient males. Therefore, efficient ways for enrichment and gene transfer into SSCs provides a powerful tool for production of transgenic animals.
Hassan, Abbasi, Sayyed Morteza, Hosseini, Mahdi, Hajian, Zahra, Nasiri, Mehrnoosh, Bahadorani, Mojtaba, Tahmoorespur, Mohammad Reza, Nasiri, Mohammad Hossein, Nasr-Esfahani   +7 more
openaire   +2 more sources

Lentiviral Transduction for Optimal LSC/HSC Manipulation

2020
Historically, efficient transduction of hematopoietic stem cells (HSC) to study the role of specific genes on HSC function, as well as to broaden the potential of gene therapy for hematopoietic related diseases has relied on our ability to design vectors capable of delivering the gene of interest without affecting HSC function.
openaire   +2 more sources

Lentiviral Transduction of Face and Limb Flaps

Plastic and Reconstructive Surgery, 2012
Ex vivo introduction of an immunomodulatory transgene into a face or hand allograft may improve the risk-to-benefit ratio of vascularized composite allografts. Abrogation of the immunogenicity of the skin component of a face or hand allograft may decrease alloreactivity and permit the induction of immunologic tolerance.
Angelo A, Leto Barone, Zhao Y, Zhou, Michael W, Hughes, Ryan, Park, Ruth M, Schulman, Steven, Lee, Evan N, Vidar, Travis L, Shiba, Erin L, Weber, Curtis L, Cetrulo   +9 more
openaire   +2 more sources