Results 271 to 280 of about 201,604 (308)
Some of the next articles are maybe not open access.
Journal of Virology, 1991
Transcriptional regulatory mechanisms found in lentiviruses employ RNA enhancer elements called trans-activation responsive (TAR) elements. These nascent RNA stem-loops are cis-acting targets of virally encoded Tat effectors. Interactions between Tat and TAR increase the processivity of transcription complexes and lead to efficient copying of viral ...
D, Derse +3 more
openaire +2 more sources
Transcriptional regulatory mechanisms found in lentiviruses employ RNA enhancer elements called trans-activation responsive (TAR) elements. These nascent RNA stem-loops are cis-acting targets of virally encoded Tat effectors. Interactions between Tat and TAR increase the processivity of transcription complexes and lead to efficient copying of viral ...
D, Derse +3 more
openaire +2 more sources
Lentivirus Production and Purification
2016Lentiviral (LV) vectors offer unique advantages over other gene delivery systems, namely the ability to integrate transgenes into the genome of both dividing and nondividing cells. Detailed herein is a simple protocol for the production LV vectors, describing the triple transfection of an LV transfer vector and LV helper plasmids into HEK-293 cells ...
Matthew J, Benskey +1 more
openaire +2 more sources
Gene delivery by lentivirus vectors
Molecular Biotechnology, 2007The capacity to efficiently transduce nondividing cells, shuttle large genetic payloads, and maintain stable long-term transgene expression are attributes that have brought lentiviral vectors to the forefront of gene delivery vehicles for research and therapeutic applications in a clinical setting.
Adam S, Cockrell, Tal, Kafri
openaire +2 more sources
SIVsmmPBj14: An Atypical Lentivirus
1994SIV-PBj14 is atypical for a lentivirus in that infection of pig-tailed macaques usually does not result in long-term progressive disease; however, this model may potentially provide valuable information about the pathogenesis of HIV and the development of AIDS. By capitalizing on some of the unique properties of the virus and the model system discussed
openaire +2 more sources
Lentivirus Vectors in β-Thalassemia
2012Patients with β-thalassemia major require lifelong transfusions and iron chelation, regardless of the type of causative mutations (e.g., β⁰, β(E)/β⁰). The only available curative therapy is allogeneic hematopoietic transplantation, although most patients do not have an HLA-matched, geno-identical donor, and those who do still risk graft-versus-host ...
Emmanuel, Payen +5 more
openaire +2 more sources
Microinjecting Lentivirus into Mouse Embryos
Cold Spring Harbor Protocols, 2018This protocol describes the injection of lentivirus into the perivitelline space of fertilized oocytes. Embryos usually survive lentiviral injection well because only the zona is pierced.
openaire +2 more sources
Lentivirus expression vectors.
2007Research Signpost Transworld Research Network, Kerala (India)
CONESE, MASSIMO +3 more
openaire +1 more source
Lentivirus Gene Engineering Protocols
2010Part I: Overview 1. Lentiviral Vector-Mediated Gene Transfer and RNA Silencing Technology in Neuronal Dysfunctions Jean-Luc Dreyer Part II: Advances in Lentiviral Vector Technology 2. New Protocol for Lentiviral Vector Mass Production Maria Mercedes Segura, Alain Garnier, Yves Durocher, Sven Ansorge, and Amine Kamen 3.
openaire +1 more source