A Lyophilizable Nanoparticle Vaccine Specific for a Novel Linear Neutralizing Epitope in the α2-α3 Helices of Domain 3 of Lethal Factor from Bacillus anthracis [PDF]
ToxinsAnthrax remains a serious bioterrorism threat for which new and thermostable vaccines are needed. We previously demonstrated that immunization of rabbits with multiple-antigenic-peptide (MAP) vaccines elicit antibody (Ab) against the loop-neutralizing ...
Jon Oscherwitz +5 more
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The Anthrax Toxin Lethal Factor in Solution Does Not Have the Protein’s Crystallized Structure [PDF]
ToxinsThe bacterium Bacillus anthracis secretes three protein exotoxins: Protective Antigen 83 (PA83), Lethal Factor (LF), and Edema Factor (EF). A cleaved form of PA83 (PA63) aids LF and EF entry into the cytoplasm, which leads to anthrax-induced cell death ...
Kenneth A. Rubinson, John J. Kasianowicz
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Asymmetric Cryo-EM Structure of Anthrax Toxin Protective Antigen Pore with Lethal Factor N-Terminal Domain [PDF]
Toxins, 2017 The anthrax lethal toxin consists of protective antigen (PA) and lethal factor (LF). Understanding both the PA pore formation and LF translocation through the PA pore is crucial to mitigating and perhaps preventing anthrax disease.
Alexandra J. Machen +10 more
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Molecular basis of specificity and deamidation of eIF4A by Burkholderia Lethal Factor 1 [PDF]
Communications Biology, 2022 The crystal structure of the toxin from the pathogenic bacterium Burkholderia pseudomallei in complex with its target, human eIF4A, provides insights into substrate specificity and may facilitate the design of inhibitors for the treatment of melioidosis.
George W. Mobbs +10 more
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Biochip for the Detection of Bacillus anthracis Lethal Factor and Therapeutic Agents against Anthrax Toxins [PDF]
Membranes, 2016 Tethered lipid bilayer membranes (tBLMs) have been used in many applications, including biosensing and membrane protein structure studies. This report describes a biosensor for anthrax toxins that was fabricated through the self-assembly of a tBLM with B.
Vitalii Silin +5 more
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Phage Display Analysis of Monoclonal Antibody Binding to Anthrax Toxin Lethal Factor [PDF]
Toxins, 2017 AVR1674 and AVR1675 are monoclonal antibodies (mAbs) that bind with high specificity to anthrax toxin lethal factor (LF) and lethal toxin (LTx).
Jason M. Goldstein +6 more
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Sequence analysis of the cDNA encoding for SpCTx: a lethal factor from scorpionfish venom (Scorpaena plumieri) [PDF]
Journal of Venomous Animals and Toxins including Tropical Diseases, 2018 Background Lethal factors are multifunctional oligomeric proteins found in the venomous apparatus of Scorpaeniformes fish. These toxins elicit not only an array of biological responses in vitro but also cardiovascular disorders and strong hemolytic ...
Fábio L. S. Costa +6 more
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Anthrax toxin translocation complex reveals insight into the lethal factor unfolding and refolding mechanism [PDF]
Scientific Reports, 2021 Translocation is essential to the anthrax toxin mechanism. Protective antigen (PA), the binding component of this AB toxin, forms an oligomeric pore that translocates lethal factor (LF) or edema factor, the active components of the toxin, into the cell ...
Alexandra J. Machen +2 more
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Inhibitors of the Metalloproteinase Anthrax Lethal Factor. [PDF]
Curr Top Med Chem, 2016 Bacillus anthracis, a rod shaped, spore forming, gram positive bacteria, is the etiological agent of anthrax. B. anthracis virulence is partly attributable to two secreted bipartite protein toxins, which act inside host cells to disrupt signaling pathways important for host defense against infection.
Goldberg AB, Turk BE.
europepmc +4 more sources
Inhibition of anthrax lethal factor by ssDNA aptamers. [PDF]
Arch Biochem Biophys, 2018 Anthrax is caused by Bacillus anthracis, a bacterium that is able to secrete the toxins protective antigen, edema factor and lethal factor. Due to the high level of secretion from the bacteria and its severe virulence, lethal factor (LF) has been sought as a biomarker for detecting bacterial infection and as an effective target to neutralize toxicity ...
Lahousse M +8 more
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