Results 241 to 250 of about 639,233 (341)

Multimodal Chromatography in the Downstream Processing of mAb‐Based Products: Mechanisms, Strategies, and Applications

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Multimodal chromatography has emerged as a powerful tool for the purification of monoclonal antibodies (mAbs) and their derivatives—including antibody fragments (Fabs), Fc‐fusions, bispecific (BsAb), and antibody–drug conjugates (ADCs)—offering enhanced selectivity through the integration of ionic, hydrophobic, hydrogen‐bonding, and π–π ...
Amin Javidanbardan, Marco P․ C․ Marques, Ana M. Azevedo, Daniel G. Bracewell, Stephen Goldrick   +4 more
wiley   +1 more source

Engineering of CoA‐Acylating Butyraldehyde Dehydrogenase for Enhanced 1,3‐Butanediol Production in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Microbial production of 1,3‐butanediol (1,3‐BDO) offers a renewable route to this versatile C4 chemical. However, the low performance of CoA‐acylating butyraldehyde dehydrogenase (Bld), which contains a catalytic cysteine, limits efficient production in recombinant Escherichia coli (E. coli).
Seunghyun Cho, Tayyab Islam, Sobia Islam, Junhak Lee, Sung Won Jung, Sunghoon Park   +5 more
wiley   +1 more source

RFC1-related disorders: A case series of 4-aminopyridine and acetyl-DL-leucine treatment. [PDF]

Cerebellum
Heindl F, Traschütz A, Synofzik M, Wirth T, Anheim M, Tarnutzer AA, Hartmann AM, Rujescu D, Giegling I, Feil K, Adrion C, Strupp M.   +11 more
europepmc   +1 more source

Degradation of mitochondrial translation products in Neurospora crassa [PDF]

, 1974
Bohley, P., Hanson, Horst, Neupert, Walter, Rücker, Axel von   +3 more
core  

Impact of Process Interruptions in the Production of Lysates for Cell‐Free Expression Systems

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cell‐free gene expression systems offer cell‐like functionalities outside the confines of the cell, garnering increasing interest for applications from biomanufacturing to sensing. As applications expand, the need to implement economically scaled processes to produce cellular lysates grows.
Katherine A. Rhea, Ethan N. Walters, Julie L. Zacharko, Mia J. Seergae, Thomas R. Laws, Thomas C. Maishman, Yun‐Nam Choi, John T. Lazar, Ashty Karim, Weston Kightlinger, Michael C. Jewett, Matthew W. Lux   +11 more
wiley   +1 more source

Association and predictive value of blood branched-chain and excitatory amino acids with autism spectrum disorder severity. [PDF]

BMC Pediatr
Li J, Wang Y, Bi L, Yang X, Yang Y, Zhang Q, Mei X, Dang W, Feng G, Zhang X, Zhou R.   +10 more
europepmc   +1 more source

Optimizing Pichia Pastoris Cell‐Free Protein Synthesis to Improve Economics

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cell‐free protein synthesis (CFPS) is a powerful and versatile platform that supports a wide range of applications, from fundamental studies of the genetic code to scalable and rapid protein production. The recently developed Pichia pastoris CFPS combines advantages of both prokaryotic and eukaryotic systems, including a rapid growth rate ...
Rui Wu, Igor Koop, Gavin Murugan, Thagshanen Thillairajah, Karen M. Polizzi   +4 more
wiley   +1 more source

From Classics to Nano-Excipients and Biopolymers: Pulmonary Drug Delivery Formulations. [PDF]

Pharmaceutics
Nikou M, Chountoulesi M, Pispas S, Pippa N.   +3 more
europepmc   +1 more source

Advancing design strategies in smart stimulus‐responsive liposomes for drug release and nanomedicine

BMEMat, EarlyView.
Schematic illustration of stimulus‐responsive liposomes designed for controlled drug release and nanomedicine. The innermost circle represents different liposomal structures, including unilamellar, multilamellar, and multivesicular liposomes. The middle layer illustrates the responsive phospholipid components.
Yuchen Guo, Nan Li, Daiwei Zhang, Jingya Gu, Zhihuan Liao, Zihao Teng, Xiangfu Du, Peter S. Timashev, Shipeng Chen, Shuaidong Huo   +9 more
wiley   +1 more source