Results 61 to 70 of about 16,604,758 (298)

A comparison of in vivo viral targeting systems identifies adeno‐associated virus serotype 9 (AAV9) as an effective vector for genetic manipulation of Leydig cells in adult mice

, 2021
Background: Despite the increasing popularity of deliverable transgenics, a robust and fully validated method for targeting Leydig cells, capable of delivering long-term transgene expression, is yet to be defined.
Milne, L, Natalie Reed, Lee B. Smith, Pamela Brown, Darbey, A, Jeffery, N, Annalucia Darbey, Diane Rebourcet, Nathan Jeffery, Curley, M, Smith, LB, Rebourcet, D, Cornelia Roesl, Michael Curley, Karen Kilcoyne, Brown, P, Kilcoyne, K, Laura Milne, Reed, N, Roesl, C   +19 more
core   +1 more source

Functional Sex Reversal in Adult Gonochoristic Teleosts is Mediated by Ovarian Germline Stem Cell Plasticity and Somatic Cell Transdifferentiation

Advanced Science, EarlyView.
Oocytes play crucial roles in maintaining ovarian fate in adult gonochoristic fish. Targeted oocyte ablation with aromatase inhibition triggers the activation of surviving germline stem cells and the transdifferentiation of somatic cells from granoulosa/theca to Sertoli/Leydig lineages, inducing a complete and functional female‐to‐male sex reversal ...
Yang Yang, Liechao Yu, Wanchang Zhang, Gary Wessel, Ruiyi Chen, Weihua Hu, Dongdong Xu   +6 more
wiley   +1 more source

Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells

International Journal of Molecular Sciences, 2019
Zinc oxide nanoparticles (ZnO NPs) have shown adverse health impact on the human male reproductive system, with evidence of inducing apoptosis. However, whether or not ZnO NPs could promote autophagy, and the possible role of autophagy in the progress of
J. Shen, Dan Yang, Xingfan Zhou, Yu-Qian Wang, Shichuan Tang, Hong Yin, Jing-Lei Wang, Rui Chen, Jia-Xiang Chen   +8 more
semanticscholar   +1 more source

Grtp1 Safeguards Against Paternal Reproductive Aging and Intergenerational Behavioral Deficits by Preserving Redox Homeostasis

Advanced Science, EarlyView.
This study identifies that Grtp1 maintains germline redox homeostasis by interacting with PRDX1/4‐TXN to antagonize paternal reproductive aging. Growth hormone restores Grtp1 expression, rectifies aberrant sperm DNA methylation, and ameliorates intergenerational anxiety and social deficits, highlighting the GH‐GRTP1 axis as a promising intervention ...
Yingdong Liu, Shanyao Liu, Haixin Liang, Jiani Yang, Yanhe Li, Jiqing Yin, Xueguang Zhang, Yan Zhang, Dandan Bai, Zihui Yan, Zhuoao Zhang, Yanping Jia, Kuisheng Liu, Yifan Sheng, Jiani Xiang, Chenxiang Xi, Baoxing Dong, Xinyi Lei, Jiayu Chen, Hong Wang, Yi Guo, Yanping Zhang, Shaorong Gao, Wenqiang Liu   +23 more
wiley   +1 more source

DUET‐seq: An Open‐Source Droplet Platform for High‐Fidelity Joint Chromatin and Transcriptome Profiling Reveals Temporal Regulatory Decoupling in Single Cells

Advanced Science, EarlyView.
DUET‐seq is an open‐source droplet platform that jointly profiles chromatin accessibility and gene expression from the same nucleus. Dissolvable dual‐linker hydrogel beads and a one‐step intra‐droplet RT‐PCR co‐index both modalities in under 12 h at roughly $0.04 per cell.
Dong Cheng, Zijun Meng, Lan Wei, Pingjing Yang, Fanfan Zhang, Zhiyi Luo, Ting Lai, Chunli Li, Mengyao Zhao, Mengqin Xu, Jiaqi Wang, Linjun Li, Huarong Chen, Jin Li, Ailong Huang, Youquan Bu, Liuyang Zhao   +16 more
wiley   +1 more source

Progenitor cells of the testosterone-producing Leydig cells revealed [PDF]

, 2004
The cells responsible for production of the male sex hormone testosterone, the Leydig cells of the testis, are post-mitotic cells with neuroendocrine characteristics.
Michail S. Davidoff, Dieter Riethmacher, Davidoff, M.S., Muller, D., Adolf F. Holstein, Middendorff, R., Holstein, A. F., Holstein, A.F., Dieter Müller, Enikolopov, G. N., Riethmacher, D., Davidoff, M. S., Müller, D., Enikolopov, G., Grigori Enikolopov, Middendorff, R., Riethmacher, D., Ralf Middendorff   +17 more
core   +1 more source

Specific Transcriptomic Signatures and Dual Regulation of Steroidogenesis Between Fetal and Adult Mouse Leydig Cells [PDF]

, 2021
Leydig cells (LC) are the main testicular androgen-producing cells. In eutherian mammals, two types of LCs emerge successively during testicular development, fetal Leydig cells (FLCs) and adult Leydig cells (ALCs). Both display significant differences in
Serge Nef, Neirijnck, Yasmine, Sararols, P, Nef, S, Stevant, Isabelle, Isabelle Stévant, Neirijnck, Y, Stévant, I, Pauline Sararols, Françoise Kühne, Nef, Serge, Dermitzakis, E, Rebourcet, Diane, Darbey, Annalucia, Emmanouil Dermitzakis, Kuhne, Françoise, Lee B. Smith, Darbey, A, Kühne, F, Michael K. Curley, Dermitzakis, Emmanouil, Annalucia Darbey, Curley, MK, Diane Rebourcet, Smith, LB, Rebourcet, D, Sararols, Pauline, Smith, Lee B, Yasmine Neirijnck, Curley, Michael K   +29 more
core   +1 more source

The in vitro modulation of steroidogenesis by inflammatory cytokines and insulin in TM3 Leydig cells

Reproductive Biology and Endocrinology, 2018
BackgroundCytokines and hormones, including insulin, are known to modulate the hypothalamic-pituitary-testes axis and steroidogenesis, both centrally and peripherally.
K. Leisegang, R. Henkel
semanticscholar   +1 more source

Evaluation of therapeutic effects of D‐limonene following orchiopexy in the rat model of cryptorchidism

Animal Models and Experimental Medicine, EarlyView.
D‐limonene supplementation enhances testicular recovery following orchiopexy in a rat model of bilateral cryptorchidism. Treatment with D‐limonene reduces oxidative stress (↓MDA, ↑SOD, ↑GPx, ↑TAC), downregulates pro‐apoptotic proteins (↓TNF‐α, ↓BAX, ↓Caspase‐3), and upregulates anti‐apoptotic Bcl‐2 expression.
Arman Norouzi‐Ghalehbala, Mohammadjavad Rezaei‐Varmaziar, Majid Taati, Ali Soleimanzadeh, Abbas Raisi, Farshid Davoodi, Amir Ali Shahbazfar   +6 more
wiley   +1 more source

Androgen receptor expression is required to ensure development of adult Leydig cells and to prevent development of steroidogenic cells with adrenal characteristics in the mouse testis [PDF]

, 2019
Background: The interstitium of the mouse testis contains Leydig cells and a small number of steroidogenic cells with adrenal characteristics which may be derived from the fetal adrenal during development or may be a normal subset of the developing ...
O'Hara, Laura, Claahsen-van der Grinten, Hedi, O'Shaughnessy, Peter J, Smith, Lee, Mitchell, R.T., Brown, Pamela, Claahsen-van der Grinten, H.L., O'Hara, Laura; id_orcid, Brown, Pamela; id_orcid, Smith, L.B., Cruickshanks, Lyndsey, Mitchell, Rod T, Mitchell, Rod T., Monteiro, Ana, Abel, Margaret, O'Shaughnessy, Peter J., Claahsen-van der Grinten, Hedi L, Smith, Lee B, Mitchell, Roderick, Smith, Lee B.   +19 more
core   +2 more sources