Results 241 to 250 of about 104,318 (277)

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Journal of Visualized Experiments, 2022
Deafness is the most common sensory impairment, affecting approximately 5% or 430 million people worldwide as per the World Health Organization1. Aging or presbycusis is a primary cause of sensorineural hearing loss and is characterized by damage to hair cells, spiral ganglion neurons (SGNs), and the stria vascularis. These structures reside within the
Peter A, Santi, Shane B, Johnson
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Volume holographic optical element for light sheet fluorescence microscopy

Optics Letters, 2020
Three-dimensional (3D) imaging of living organisms requires fine optical sectioning and high-speed image acquisition, which can be achieved by light sheet fluorescence microscopy (LSFM). However, orthogonal illumination and detection arms in the LSFM system make it bulky.
Ting-Yu, Hsieh, Sunil, Vyas, Jui-Ching, Wu, Yuan, Luo   +3 more
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Light-sheet fluorescent microscopy: fundamentals, developments and applications

Physica Scripta, 2023
Abstract Deep volumetric microscopy of live objects plays a critical role in biology and medicine. To this end, development of rapid and non-invasive optical methods for 3-dimensional (3D) imaging is still demanding. In this way, light-sheet fluorescence microscopy (LSFM) has emerged as a volumetric microscopy method having high spatial ...
Hosein Kafian, Sina Mozaffari-Jovin, Mohammad Bagheri, Seyed Ali Mousavi Shaegh   +3 more
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Light-sheet fluorescence microscopy for quantitative biology

Nature Methods, 2014
In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.
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Metalens-based light-sheet fluorescence microscopy

Optica Imaging Congress 2025 (3D, DH, COSI, IS, pcAOP,RadIT)
Metalens-based dual-sided illumination light-sheet fluorescence microscopy utilizes paired cylindrical metalenses to generate uniform twin light sheets, eliminating shadow artifacts. The mouse lung tissues are measured by the system to offer high-resolution and sectioning imaging results.
Hung-Chuan Hsu, Chun Chun Chang, Bo-Wei Huang, Sunil Vyas, S-Ja Tseng, Cheng Hung Chu, Kuang-Yuh Huang, Takuo Tanaka, Din Ping Tsai, Yuan Luo   +9 more
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Light sheet fluorescence microscopy with active optical manipulation

Optics Letters
We present a light sheet fluorescence microscopy (LSFM) with active optical manipulation by using linear optical tweezers (LOTs). In this method, two coaxially transmitting laser beams of different wavelengths are shaped using cylindrical lenses to form a linear optical trapping perpendicular to the optical axis and an excitation light sheet (LS ...
Jun, Yin, Ruijing, Liang, Haoyi, Hou, Yan, Miao, Lingyao, Yu   +4 more
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Light sheet fluorescence microscopy (LSFM): past, present and future

The Analyst, 2014
Light sheet fluorescence microscopy (LSFM) has emerged as an important imaging modality to follow biology in live 3D samples over time with reduced phototoxicity and photobleaching.
John, Lim, Hwee Kuan, Lee, Weimiao, Yu, Sohail, Ahmed   +3 more
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Multimodal light-sheet microscopy for fluorescence live imaging

SPIE Proceedings, 2012
Light-sheet microscopy, it is known as single plane illumination microscope (SPIM), is a fluorescence imaging technique which can avoid phototoxic effects to living cells and gives high contrast and high spatial resolution by optical sectioning with light-sheet illumination in developmental biology.
Y. Oshima, H. Kajiura-Kobayashi, S. Nonaka   +2 more
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Smartphone light sheet fluorescence microscopy for molecular diagnostics

Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP), 2019
A new design and implementation of smartphone light sheet microscopy based on inkjet-printed DotLens is presented. Its ultimate simplicity, yet high quality imaging and sensing capabilities would find applications in point-of-care diagnostics and low-resources scenarios.
Hoang Nguyen, Wei-Chuan Shih
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Light-Sheet Fluorescence Microscopy

2022
George O. T. Merces, Emmanuel G. Reynaud
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