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, 2014 This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisition, minimal phototoxicity, and multiview sample ...
Weber, M., Mickoleit, M., Huisken, J.
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Quantifiable Intravital Light Sheet Microscopy
2022Live imaging of zebrafish embryos that maintains normal development can be difficult to achieve due to a combination of sample mounting, immobilization, and phototoxicity issues that, once overcome, often still results in image quality sufficiently poor that computer-aided analysis or even manual analysis is not possible. Here, we describe our mounting
Holly C, Gibbs +7 more
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An eye on light-sheet microscopy
2016This chapter introduces the principles and advantages of selective plane illumination microscopy (SPIM) and compares it to commonly used epifluorescence or confocal setups. Due to the low phototoxicity, speed of imaging, high penetration depth, and spatiotemporal resolution, SPIM is predestined for in vivo imaging but can as well be used for in toto ...
D. Kromm, T. Thumberger, J. Wittbrodt
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Single-objective light sheet microscopy
Nature Methods, 2021Single-objective light sheet fluorescence microscopes are driving innovation in volumetric imaging.
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OpenSPIM: an open-access light-sheet microscopy platform
Nature Methods, 2013 Light-sheet microscopy is revolutionizing biology by enabling live in toto imaging of entire embryos or organs with minimal phototoxicity1. We present an open hardware and software platform for constructing a customizable microscope for selective- plane ...
Johannes Schindelin +2 more
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Fast Fluorescence Microscopy with Light Sheets
The Biological Bulletin, 2016In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast full-frame acquisition. This illumination scheme provides minimal photobleaching and phototoxicity. Complemented with remote focusing and multi-view acquisition, light sheet microscopy is the method of choice for acquisition of
Daetwyler, S., Huisken, J.
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A Practical Guide to Light Sheet Microscopy
2016Light sheet fluorescence microscopy is an efficient method for imaging large volumes of biological tissue, including brains of larval zebrafish, at high spatial and fairly high temporal resolution with minimal phototoxicity.Here, we provide a practical guide for those who intend to build a light sheet microscope for fluorescence imaging in live larval ...
Davis V, Bennett, Misha B, Ahrens
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Resolution upgrades for light-sheet microscopy
Nature Methods, 2019Single objective light-sheet fluorescence microscopes combine the convenience of conventional sample mounting with sensitive subcellular and super-resolution imaging of cells and tissues.
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Light Sheet Microscopy in Cell Biology
2012Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching, and good optical penetration depth.
Raju, Tomer +2 more
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Light Sheet Microscopy and Imaging
2021Light Sheet Microscopy and Imaging introduces the fundamentals and important advancements at the frontiers of optical physics and applied sciences. It presents coherent, in-depth explanations of different variants of light sheet microscopes beginning from the basics of light.
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