Results 51 to 60 of about 36,659 (240)

Thin-sheet laser imaging microscopy for optical sectioning of thick tissues

BioTechniques, 2009
We report the development of a modular and optimized thin-sheet laser imaging microscope (TSLIM) for nondestructive optical sectioning of organisms and thick tissues such as the mouse cochlea, zebrafish brain/inner ear, and rat brain at a resolution that
Peter A. Santi, Shane B. Johnson, Matthias Hillenbrand, Patrick Z. Grandpre, Tiffany J. Glass, James R. Leger   +5 more
doaj   +1 more source

Biomimetic Iridescent Skin: Robust Prototissues Spontaneously Assembled from Photonic Protocells

Advanced Functional Materials, EarlyView.
Uniform nanoparticles are induced to form arrays (photonic crystals) in the cores of biopolymer capsules, endowing these ‘protocells’ with structural color. These protocells are then assembled into large self‐standing objects, i.e., prototissues, with robust mechanical properties as well as iridescent optical properties.
Medha Rath, Briahnah Streeter, Satyam Srivastava, Jemma Wood, William E. Bentley, Taylor J. Woehl, Srinivasa R. Raghavan   +6 more
wiley   +1 more source

Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae

Scientific Reports, 2021
Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, multiphoton and light sheet microscopy.
Dimitrios Kapsokalyvas, Rodrigo Rosas, Rob W. A. Janssen, Jo M. Vanoevelen, Miranda Nabben, Martin Strauch, Dorit Merhof, Marc A. M. J. van Zandvoort   +7 more
doaj   +1 more source

GeAl2‐2xFe2xO3(OH)4 Nanotubes: New Electrocatalyst for Oxygen Evolution Reaction

Advanced Functional Materials, EarlyView.
Fe‐doped imogolite nanotubes are synthesized via a one‐step hydrothermal method with varying Fe substitution ratios x. Structural and spectroscopic analyses confirm homogeneous Fe incorporation while preserving tubular shape. Optimal doping at x = 0.05 enhances optical absorption, narrows band gap, reduces charge transfer resistance, and significantly ...
Yassine Naciri, Jian Li, Wahid Ullah, Fabien Baron, Franck Bourdelle, Stéphan Rouzière, Claire Goldmann, Erik Elkaim, Thomas Bizien, Rachid Belkhou, Delphine Vantelon, Mohamed Nawfal Ghazzal, Erwan Paineau   +12 more
wiley   +1 more source

Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy

eLife, 2020
As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models.
Claudia Prahst, Parham Ashrafzadeh, Thomas Mead, Ana Figueiredo, Karen Chang, Douglas Richardson, Lakshmi Venkaraman, Mark Richards, Ana Martins Russo, Kyle Harrington, Marie Ouarné, Andreia Pena, Dong Feng Chen, Lena Claesson-Welsh, Kin-Sang Cho, Claudio A Franco, Katie Bentley   +16 more
doaj   +1 more source

Light-sheet fluorescence microscopy for biomedicine

, 2023
The research motivation behind this thesis is to expand the information that can currently be extracted from an ex vivo biological sample via light-sheet fluorescence microscopy (LSFM) by enhancing the achievable resolution at an increased penetration depth. LSFM has already been used considerably for various biomedical research applications.
openaire   +3 more sources

Monitoring microbial communities using light sheet fluorescence microscopy [PDF]

Current Opinion in Microbiology, 2017
Microbes often live in dense, dynamic, multi-species communities whose architecture and function are intimately intertwined. Imaging these complex, three-dimensional ensembles presents considerable technical challenges, however. In this review, I describe light sheet fluorescence microscopy, a technique that enables rapid acquisition of three ...
openaire   +2 more sources

Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets [PDF]

Scientific Reports, 2016
AbstractWe demonstrate a simple and efficient method for producing ultrathin Bessel (‘non-diffracting’) light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm and high-speed, 4D volume ...
Zhao, Teng, Lau, Sze Cheung, Wang, Ying, Su, Yumian, Wang, Hao, Cheng, Aifang, Herrup, Karl, Ip, Nancy Y., Du, Shengwang, Loy, M.M.T.   +9 more
openaire   +3 more sources

Multifunctional Microstructured Surfaces by Microcontact Printing of Reactive Microgels

Advanced Functional Materials, EarlyView.
Reactive poly(N‐vinylcaprolactam‐co‐glycidyl methacrylate) microgels are used as functional inks to create surface‐grafted arrays on glass via microcontact printing. The patterns (10–50 µm widths and spacings) enable stable binding and post‐functionalization with dyes and peptides.
Inga Litzen, Alexander Töpel, Martin Zenke, Antonio Sechi, Andrij Pich   +4 more
wiley   +1 more source

Beam shaping in light-sheet microscopy: an experimental analysis

JPhys Photonics
Thanks to its unique optical sectioning capability, light-sheet fluorescence microscopy has proven to be a powerful technique for volumetric imaging of entire model organisms with high spatial and temporal resolution.
Manuel Hüpfel, Gerd Ulrich Nienhaus
doaj   +1 more source