Results 91 to 100 of about 13,725 (250)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Invertible Linear Ordinary Differential Operators Represented as a Composition of the Simplest Operators

open access: yesMathematics and Mathematical Modeling, 2018
This article is a sequel to the earlier articles, which describe the invertible ordinary differential operators and their generalizations. The generalizations are invertible mappings of filtered modules generated by one differentiation, and are called invertible D-operators.
openaire   +2 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Smoothing technique for nonsmooth composite minimization with linear operator

open access: yes, 2017
26 pages, 5 ...
Van Nguyen, Quang   +2 more
openaire   +2 more sources

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

open access: yesFEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada   +5 more
wiley   +1 more source

Compact Linear Combination of Composition Operators on Bergman Spaces

open access: yesJournal of Research in Applied Mathematics
Motivated by the question of Shapiro and Sundberg raised in 1990, study on linear combinations of composition operators has been a topic of growing interest. In this paper, we completely characterize the compactness of any finite linear combination of composition operators with general symbols on the weighted Bergman spaces in two classical terms: one ...
Kang, Qijian, Wang, Maofa
openaire   +3 more sources

Compact linear combinations of composition operators on Hardy spaces

open access: yesArchiv der Mathematik
Let $φ_j$, $j=1,2, \dots, N$, be holomorphic self-maps of the unit disk $\mathbb{D}$ of $\mathbb{C}$. We prove that the compactness of a linear combination of the composition operators $C_{φ_j}: f\mapsto f\circφ_j$ on the Hardy space $H^p(\mathbb{D})$ does not depend on $p$ for ...
Evgueni Doubtsov, Dmitry V. Rutsky
openaire   +3 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

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