Results 91 to 100 of about 13,725 (250)
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
This article is a sequel to the earlier articles, which describe the invertible ordinary differential operators and their generalizations. The generalizations are invertible mappings of filtered modules generated by one differentiation, and are called invertible D-operators.
openaire +2 more sources
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Smoothing technique for nonsmooth composite minimization with linear operator
26 pages, 5 ...
Van Nguyen, Quang +2 more
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Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada +5 more
wiley +1 more source
Compact Linear Combination of Composition Operators on Bergman Spaces
Motivated by the question of Shapiro and Sundberg raised in 1990, study on linear combinations of composition operators has been a topic of growing interest. In this paper, we completely characterize the compactness of any finite linear combination of composition operators with general symbols on the weighted Bergman spaces in two classical terms: one ...
Kang, Qijian, Wang, Maofa
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Compact linear combinations of composition operators on Hardy spaces
Let $φ_j$, $j=1,2, \dots, N$, be holomorphic self-maps of the unit disk $\mathbb{D}$ of $\mathbb{C}$. We prove that the compactness of a linear combination of the composition operators $C_{φ_j}: f\mapsto f\circφ_j$ on the Hardy space $H^p(\mathbb{D})$ does not depend on $p$ for ...
Evgueni Doubtsov, Dmitry V. Rutsky
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Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source

