Results 181 to 190 of about 327,843 (358)

In Vivo Biosynthesis and Direct Incorporation of Noncanonical Amino Acids into Proteins

open access: yesChemBioChem, EarlyView.
This review evaluates engineered (semi)autonomous cell systems for the biosynthesis and incorporation of noncanonical amino acids (ncAAs) into proteins. While semi‐autonomous cells convert supplied precursors into ncAAs autonomous cells integrate biosynthetic pathways that produce these building blocks intracellularly.
Jan Hendrik Illies   +2 more
wiley   +1 more source

Reaction Engineering of In Vitro Natural Product Biosynthesis: Challenges and Strategies

open access: yesChemBioChem, EarlyView.
The yield and turnover of in vitro natural product biosynthesis, and even its feasibility, are determined by intermolecular interactions. Reaction engineering allows to improve the performance of such complex biocatalytic systems. Natural products are widely used as pharmaceuticals and agrochemicals, or as active ingredients in food and cosmetics ...
Elsa Sánchez‐García   +2 more
wiley   +1 more source

GCI‐Based Affinity Screening of Synthetic Oligomannosides toward Concanavalin A

open access: yesChemistry – A European Journal, EarlyView.
This study describes the development of a reliable GCI method for the affinity determination of neo‐glycoproteins toward concanavalin A (ConA), a model lectin. The final goal is to investigate multivalent carbohydrate–lectin interactions. Abstract Herein, we report the first application of grating‐coupled interferometry (GCI) to quantify the binding ...
Davide Rubes   +9 more
wiley   +1 more source

Total Synthesis and Analysis of Phenolic Phytoprostanes, Oxidized Derivatives of Lipophenols

open access: yesChemistry – A European Journal, EarlyView.
The total synthesis of four hydroxytyrosol‐phytoprostanes (PhytoPs‐HT) was achieved in 20 steps, enabling their first identification as HT‐α‐linolenate oxidation products. This work revealed new oxidized derivatives of lipophenols via UHPLC‐MS/MS analysis, offering a strategy to trace HT‐derived compounds in complex matrices, aiding food and biomedical
M. Jordan Lehoux   +6 more
wiley   +1 more source

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