Engineered bacterial lipoate protein ligase A (lplA) restores lipoylation in cell models of lipoylation deficiency. [PDF]
J Biol ChemProtein lipoylation, a vital lysine post-translational modification, plays a crucial role in the function of key mitochondrial tricarboxylic acid cycle enzymatic complexes. In eukaryotes, lipoyl post-translational modification synthesis occurs exclusively through de novo pathways, relying on lipoyl synthesis/transfer enzymes, dependent upon ...
Bick NR +7 more
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Lipoic Acid Metabolism as a Potential Chemotherapeutic Target Against Plasmodium falciparum and Staphylococcus aureus [PDF]
Frontiers in Chemistry, 2021 Lipoic acid (LA) is an organic compound that plays a key role in cellular metabolism. It participates in a posttranslational modification (PTM) named lipoylation, an event that is highly conserved and that occurs in multimeric metabolic enzymes of very ...
Sun Liu Rei Yan +4 more
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Knockout studies reveal an important role of Plasmodium lipoic acid protein ligase A1 for asexual blood stage parasite survival. [PDF]
PLoS ONE, 2009 Lipoic acid (LA) is a dithiol-containing cofactor that is essential for the function of alpha-keto acid dehydrogenase complexes. LA acts as a reversible acyl group acceptor and 'swinging arm' during acyl-coenzyme A formation.
Svenja Günther +2 more
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Glycine Cleavage System H Protein Is Essential for Embryonic Viability, Implying Additional Function Beyond the Glycine Cleavage System [PDF]
Frontiers in Genetics, 2021 Glycine cleavage system H protein (GCSH) is a component of the glycine cleavage system (GCS), a conserved protein complex that acts to decarboxylate glycine.
Kit-Yi Leung +4 more
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The amidase domain of lipoamidase specifically inactivates lipoylated proteins in vivo. [PDF]
PLoS ONE, 2009 BackgroundIn the 1950s, Reed and coworkers discovered an enzyme activity in Streptococcus faecalis (Enterococcus faecalis) extracts that inactivated the Escherichia. coli and E.
Maroya D Spalding, Sean T Prigge
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Quantitative study of H protein lipoylation of the glycine cleavage system and a strategy to increase its activity by co-expression of LplA [PDF]
Journal of Biological Engineering, 2019 Glycine cleavage system (GCS) plays a key role in one-carbon (C1) metabolism related to the biosynthesis of a number of key intermediates with significance in both biomedicine and biotechnology.
Xinyi Zhang +4 more
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Iron overload exaggerates renal ischemia-reperfusion injury by promoting tubular cuproptosis via interrupting function of LIAS [PDF]
Redox BiologyRenal ischemia-reperfusion injury (RIRI), a major contributor to acute kidney injury (AKI) and delayed graft function (DGF), is closely associated with dysregulation of metal ion homeostasis.
Siyue Chen +6 more
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ABHD11 maintains 2-oxoglutarate metabolism by preserving functional lipoylation of the 2-oxoglutarate dehydrogenase complex [PDF]
Nature Communications, 2020 2-oxoglutarate links mitochondrial metabolism to oxygen sensing, gene transcription and epigenetic modifications. Here, the authors describe a mutagenesis screen to find genes that control 2-oxoglutarate levels, and identify ABHD11 as a mitochondrial ...
Peter S. J. Bailey +8 more
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Fruit-specific overexpression of lipoyl synthase increases both bound and unbound lipoic acid and alters the metabolome of tomato fruits [PDF]
Frontiers in Plant ScienceIntroductionLipoic acid (LA) is a key, yet overlooked player in primary metabolism, due to its role as a cofactor for various multi enzymatic complexes such as the E2 subunits of pyruvate dehydrogenase (PDH) and alpha-ketoglutarate dehydrogenase (kGDH ...
María Paz Covarrubias +4 more
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CoA‐dependent activation of mitochondrial acyl carrier protein links four neurodegenerative diseases [PDF]
EMBO Molecular Medicine, 2019 PKAN, CoPAN, MePAN, and PDH‐E2 deficiency share key phenotypic features but harbor defects in distinct metabolic processes. Selective damage to the globus pallidus occurs in these genetic neurodegenerative diseases, which arise from defects in CoA ...
Roald A Lambrechts +10 more
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