Using Lipoamidase as a Novel Probe To Interrogate the Importance of Lipoylation in
mBio, 2018 Lipoate is a redox active cofactor that is covalently bound to key enzymes of oxidative metabolism. Plasmodium falciparum is auxotrophic for lipoate during the intraerythrocytic stages, but it is not known whether lipoate attachment to protein is ...
Hugo Jhun +2 more
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Assessing the prognostic and therapeutic value of cuproptosis-related genes in colon adenocarcinoma patients [PDF]
Frontiers in Cell and Developmental BiologyBackgroundColon adenocarcinoma (COAD) remains a major global health challenge with poor prognosis despite advances in treatment, underscoring the need for new biomarkers. As a novel mode of cell death, cuproptosis is thought to be potentially involved in
Zhanhui Ye +10 more
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Chemoselective and laser cleavable probes for <i>in situ</i> protein lipoylation detection by laser desorption/ionization mass spectrometry. [PDF]
Chem SciDu Q +8 more
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Lipidomic Analysis of Plastidial Octanoyltransferase Mutants of Arabidopsis thaliana [PDF]
Metabolites, 2019 Plant de novo fatty acid synthesis takes place in the plastid using acetyl-coenzyme A (acetyl-CoA) as the main precursor. This first intermediate is produced from pyruvate through the action of the plastidial pyruvate dehydrogenase complex (PDH), which ...
Raquel Martins-Noguerol +8 more
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FDX1 regulates cellular protein lipoylation through direct binding to LIAS. [PDF]
J Biol Chem, 2023 Dreishpoon MB +8 more
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Copper-mediated SEC14L3 promotes cuproptosis to inhibit hepatocellular carcinoma growth via ERK/YY1/FDX1 axis [PDF]
Communications BiologyCuproptosis, a copper-triggered cell death pathway, holds therapeutic potential for cancers, but its regulatory mechanisms in hepatocellular carcinoma (HCC) remain undefined.
Chutian Wu +10 more
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A Deep Clinical and Biochemical Characterization of a Patient With Combined Malonic and Methylmalonic Aciduria (CMAMMA). [PDF]
JIMD RepABSTRACT Combined malonic and methylmalonic aciduria (CMAMMA) is an inborn error of metabolism caused by a deficiency in mitochondrial malonyl‐CoA synthetase, the enzyme responsible for activating malonic acid (MA) to malonyl‐CoA, a precursor of lipoic acid.
Gragnaniello V +9 more
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A unique lipoylation system in the Archaea [PDF]
The FEBS Journal, 2009 Members of the 2-oxoacid dehydrogenase multienzyme complex family play a key role in the pathways of central metabolism. Post-translational lipoylation of the dihydrolipoyl acyltransferase component of these complexes is essential for their activity, the lipoyllysine moiety performing the transfer of substrates and intermediates between the different ...
Mareike G. Posner +4 more
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A globular protein exhibits rare phase behavior and forms chemically regulated orthogonal condensates in cells [PDF]
Nature CommunicationsProteins with chemically regulatable phase separation are of great interest in the fields of biomolecular condensates and synthetic biology. Intrinsically disordered proteins (IDPs) are the dominating building blocks of biomolecular condensates which ...
Jinglei Nie +9 more
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Expression and lipoylation in Escherichia coli of the inner lipoyl domain of the E2 component of the human pyruvate dehydrogenase complex [PDF]
Biochemical Journal, 1993 The dihydrolipoamide acetyltransferase subunit (E2p) of mammalian pyruvate dehydrogenase complex has two highly conserved lipoyl domains each modified with a lipoyl cofactor bound in amide linkage to a specific lysine residue. A sub-gene encoding the inner lipoyl domain of human E2p has been over-expressed in Escherichia coli.
Janet Quinn +5 more
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