Results 101 to 110 of about 6,322,636 (242)
, 2010 A novel technique, gas-assisted three-liquid-phase extraction (GATE), was proposed to improve three-liquid-phase separation of p-nitrophenol (p-NP) and o-nitrophenol (o-NP) from wastewater.
Deng, Fuli +14 more
core +1 more source
FEBS Open Bio, EarlyView.Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
, 2011 Separation of structurally similar protoberberine alkaloids from Corydalis yanhusuo W.T.Wang in reversed-phase high-performance liquid chromatography with chaotropic ...
杜娜娜 +4 more
core
Modelling of transient gas-liquid flow and pigging in pipes [PDF]
, 1999 More and more transient gas-liquid operations in pipes are being successfully applied in the oil and gas industry. Pigging in two-phase pipelines, to remove liquid accumulation or for cleaning purposes, is an important transient operation.
Lima, P. C. R.
core +2 more sources
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
FEBS Open Bio, EarlyView.Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Yield of Protein Crystallization from Metastable Liquid–Liquid Phase Separation
MoleculesPreparative protein crystallization is regarded as an economically sustainable protein purification alternative to chromatography in biotechnological downstream processing. However, protein crystallization is a not-well-understood process that is usually
Shamberia Thomas +2 more
doaj +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Interplay Between Intracellular Transport Dynamics and Liquid‒Liquid Phase Separation
Advanced ScienceLiquid‒liquid phase separation (LLPS) is a ubiquitous process in which proteins, RNA, and biomolecules assemble into membrane‐less compartments, playing important roles in many biological functions and diseases.
Ming‐Li Zhang +9 more
doaj +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source