Results 1 to 10 of about 10,769 (286)

A New Anatomy of Autophagic Clearance: On the Roles of Intrinsic Disorder in the Membrane-Less on Membrane-Encapsulated Mechanism [PDF]

Membranes
Autophagy is a carefully regulated catabolic process that utilizes assemblies of specific sets of macromolecules operating at multiple stages of the pathway.
Vladimir N. Uversky, Hana Popelka, Daniel J. Klionsky   +2 more
doaj   +2 more sources

Protein Condensates and Protein Aggregates: In Vitro, in the Cell, and In Silico

Frontiers in Bioscience-Landmark, 2023
Similar to other polypeptides and electrolytes, proteins undergo phase transitions, obeying physicochemical laws. They can undergo liquid-to-gel and liquid-to-liquid phase transitions.
Katja Venko, Eva Žerovnik
doaj   +1 more source

Condensation properties of stress granules and processing bodies are compromised in myotonic dystrophy type 1

Disease Models & Mechanisms, 2022
RNA regulation in mammalian cells requires complex physical compartmentalisation, using structures thought to be formed by liquid-liquid phase separation. Disruption of these structures is implicated in numerous degenerative diseases.
Selma Gulyurtlu, Monika S. Magon, Patrick Guest, Panagiotis P. Papavasiliou, Kim D. Morrison, Alan R. Prescott, Judith E. Sleeman   +6 more
doaj   +1 more source

A high-throughput method for exploring the parameter space of protein liquid-liquid phase separation

Cell Reports Physical Science, 2022
Summary: Numerous proteins can undergo liquid-liquid phase separation (LLPS) in various biological processes. However, it remains a challenge to systematically evaluate the LLPS ability of proteins.
Yichen Li, Jinge Gu, Cong Liu, Dan Li
doaj   +1 more source

MERS-CoV nsp1 impairs the cellular metabolic processes by selectively downregulating mRNAs in a novel granules

Virulence, 2022
MERS-CoV infection can damage the cellular metabolic processes, but the underlying mechanisms are largely unknown. Through screening, we found non-structural protein 1 (nsp1) of MERS-CoV could inhibit cell viability, cell cycle, and cell migration ...
Zhaoyi Pan, Yujie Feng, Zhihui Wang, Zhengyang Lei, Qiuju Han, Jian Zhang   +5 more
doaj   +1 more source

In-Silico Analysis of pH-Dependent Liquid-Liquid Phase Separation in Intrinsically Disordered Proteins

Biomolecules, 2022
Intrinsically disordered proteins (IDPs) are essential players in the assembly of biomolecular condensates during liquid–liquid phase separation (LLPS). Disordered regions (IDRs) are significantly exposed to the solvent and, therefore, highly influenced ...
Carlos Pintado-Grima, Oriol Bárcenas, Salvador Ventura   +2 more
doaj   +1 more source

Phase separations in oncogenesis, tumor progressions and metastasis: a glance from hallmarks of cancer

Journal of Hematology & Oncology, 2023
Liquid–liquid phase separation (LLPS) is a novel principle for interpreting precise spatiotemporal coordination in living cells through biomolecular condensate (BMC) formation via dynamic aggregation. LLPS changes individual molecules into membrane-free,
Le-Wei Zheng, Cui-Cui Liu, Ke-Da Yu
doaj   +1 more source

The Crystallization of Active Pharmaceutical Ingredients with Low Melting Points in the Presence of Liquid–Liquid Phase Separation

Crystals, 2021
Liquid–liquid phase separation (LLPS) during the crystallization of active pharmaceutical ingredients (APIs) often causes agglomeration and other quality issues in crystal products; thus, it should be avoided if possible.
Wei Han Lin, Zai-Qun Yu, Pui Shan Chow, Reginald Beng Hee Tan   +3 more
doaj   +1 more source

Study liquid–liquid phase separation with optical microscopy: A methodology review

APL Bioengineering, 2023
Intracellular liquid–liquid phase separation (LLPS) is a critical process involving the dynamic association of biomolecules and the formation of non-membrane compartments, playing a vital role in regulating biomolecular interactions and organelle ...
Xiufeng Zhang, Haoyang Li, Yue Ma, Dongping Zhong, Shangguo Hou   +4 more
doaj   +1 more source

Systematic Analysis of Molecular Characterization and Clinical Relevance of Liquid–Liquid Phase Separation Regulators in Digestive System Neoplasms

Frontiers in Cell and Developmental Biology, 2022
Background: The role of liquid–liquid phase separation (LLPS) in cancer has also attracted more and more attention, which is found to affect transcriptional regulation, maintaining genomic stability and signal transduction, and contribute to the ...
Yaxin Zhang, Jie Li, Dan Feng, Xiaobo Peng, Bin Wang, Ting Han, Yingyi Zhang   +6 more
doaj   +1 more source