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A Systematic Evaluation of Protein Phase Separation Predictors across Diverse Protein Landscapes [PDF]
Computational and Structural Biotechnology JournalBackground: Liquid–liquid phase separation (LLPS) plays a central role in cellular regulation, with its dysregulation linked to numerous diseases. LLPS is also increasingly implicated in various biological contexts, such as virus replication.
Kate E. Gilroy, John N. Barr
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A New Anatomy of Autophagic Clearance: On the Roles of Intrinsic Disorder in the Membrane-Less on Membrane-Encapsulated Mechanism [PDF]
MembranesAutophagy is a carefully regulated catabolic process that utilizes assemblies of specific sets of macromolecules operating at multiple stages of the pathway.
Vladimir N. Uversky +2 more
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Frontiers in Molecular Biosciences, 2021 Liquid-liquid phase separation (LLPS) is a burgeoning concept in cell biology, which was associated with miRISC machinery. However, most studies about LLPS are based on overexpression of core proteins, which is far away from nature condition of cells ...
Li Min, Da Qin, Lingye Zhang
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Protein Condensates and Protein Aggregates: In Vitro, in the Cell, and In Silico
Frontiers in Bioscience-Landmark, 2023 Similar to other polypeptides and electrolytes, proteins undergo phase transitions, obeying physicochemical laws. They can undergo liquid-to-gel and liquid-to-liquid phase transitions.
Katja Venko, Eva Žerovnik
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Disease Models & Mechanisms, 2022 RNA regulation in mammalian cells requires complex physical compartmentalisation, using structures thought to be formed by liquid-liquid phase separation. Disruption of these structures is implicated in numerous degenerative diseases.
Selma Gulyurtlu +6 more
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A high-throughput method for exploring the parameter space of protein liquid-liquid phase separation
Cell Reports Physical Science, 2022 Summary: Numerous proteins can undergo liquid-liquid phase separation (LLPS) in various biological processes. However, it remains a challenge to systematically evaluate the LLPS ability of proteins.
Yichen Li, Jinge Gu, Cong Liu, Dan Li
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Virulence, 2022 MERS-CoV infection can damage the cellular metabolic processes, but the underlying mechanisms are largely unknown. Through screening, we found non-structural protein 1 (nsp1) of MERS-CoV could inhibit cell viability, cell cycle, and cell migration ...
Zhaoyi Pan +5 more
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Biomolecules, 2022 Intrinsically disordered proteins (IDPs) are essential players in the assembly of biomolecular condensates during liquid–liquid phase separation (LLPS). Disordered regions (IDRs) are significantly exposed to the solvent and, therefore, highly influenced ...
Carlos Pintado-Grima +2 more
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Journal of Hematology & Oncology, 2023 Liquid–liquid phase separation (LLPS) is a novel principle for interpreting precise spatiotemporal coordination in living cells through biomolecular condensate (BMC) formation via dynamic aggregation. LLPS changes individual molecules into membrane-free,
Le-Wei Zheng, Cui-Cui Liu, Ke-Da Yu
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Crystals, 2021 Liquid–liquid phase separation (LLPS) during the crystallization of active pharmaceutical ingredients (APIs) often causes agglomeration and other quality issues in crystal products; thus, it should be avoided if possible.
Wei Han Lin +3 more
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