Results 41 to 50 of about 10,769 (286)

Molecular mechanisms and cellular functions of liquid-liquid phase separation during antiviral immune responses

Frontiers in Immunology, 2023
Spatiotemporal separation of cellular components is vital to ensure biochemical processes. Membrane-bound organelles such as mitochondria and nuclei play a major role in isolating intracellular components, while membraneless organelles (MLOs) are ...
Shuai Yang, Shuai Yang, Weishan Shen, Jiajia Hu, Sihui Cai, Chenqiu Zhang, Shouheng Jin, Xiangdong Guan, Jianfeng Wu, Yaoxing Wu, Yaoxing Wu, Jun Cui, Jun Cui   +12 more
doaj   +1 more source

Evaluating LLP Methods: Challenges and Approaches

CoRR, 2023
Learning from Label Proportions (LLP) is an established machine learning problem with numerous real-world applications. In this setting, data items are grouped into bags, and the goal is to learn individual item labels, knowing only the features of the data and the proportions of labels in each bag.
Gabriel Franco, Giovanni Comarela, Mark Crovella   +2 more
openaire   +2 more sources

Phase Separation, Material State, and Condensate Fate in Mammalian Autophagy. [PDF]

Adv Sci (Weinh)
ABSTRACT Biomolecular phase separation has emerged as a key organizing principle in macroautophagy (hereafter autophagy). In mammalian cells, phase‐separated condensates not only serve as substrates for selective degradation, but also act as dynamic platforms for cargo recognition, signaling integration, and autophagosome assembly.
Lin Y, Dai M, Dai E, Kang R, Klionsky DJ, Tang D, Sun Y, Lv N, Jiang J.   +8 more
europepmc   +2 more sources

The background acts as a buffer of free subunits for LLPS-dominated assembly.

, 2023
The plot shows the concentration of subunits in the background, , as a function of the maximum capsid formation rate (maximized over time at a given set of parameter values) for assembly with LLPS (black ‘∘’ symbols) and without LLPS (red symbols).
Michael F. Hagan (2109034), Farzaneh Mohajerani (5574791)   +1 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

LLPS vs. LLCPS: analogies and differences

Soft Matter, 2023
We compare the process of Liquid–Liquid Phase Separation (LLPS) of flexible macromolecules, with the Liquid–Liquid Crystalline Phase Separation (LLCPS) of rigid fibrils, which involves the formation of a liquid phase with a directional alignment.
Paride Azzari, Raffaele Mezzenga
openaire   +3 more sources

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Spatiotemporal deciphering of dynamic the FUS interactome during liquid-liquid phase separation in living cells

Nature Communications
Liquid-liquid phase separations (LLPS) are membraneless organelles driven by biomolecule assembly and are implicated in cellular physiological activities.
Sunfengda Song, Haiyang Xie, Qingwen Wang, Xinyi Sun, Jiasu Xu, Rui Chen, Yuankang Zhu, Lai Jiang, Xianting Ding   +8 more
doaj   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source