Results 31 to 40 of about 7,152 (243)

Molecular mechanisms and cellular functions of liquid-liquid phase separation during antiviral immune responses

Frontiers in Immunology, 2023
Spatiotemporal separation of cellular components is vital to ensure biochemical processes. Membrane-bound organelles such as mitochondria and nuclei play a major role in isolating intracellular components, while membraneless organelles (MLOs) are ...
Shuai Yang, Shuai Yang, Weishan Shen, Jiajia Hu, Sihui Cai, Chenqiu Zhang, Shouheng Jin, Xiangdong Guan, Jianfeng Wu, Yaoxing Wu, Yaoxing Wu, Jun Cui, Jun Cui   +12 more
doaj   +1 more source

LLPSvs.LLCPS: analogies and differences

Soft Matter, 2023
We compare the process of Liquid–Liquid Phase Separation (LLPS) of flexible macromolecules, with the Liquid–Liquid Crystalline Phase Separation (LLCPS) of rigid fibrils, which involves the formation of a liquid phase with a directional alignment.
Paride Azzari, Raffaele Mezzenga
openaire   +3 more sources

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Liquid–liquid phase separation and morphologies in organic particles consisting of α-pinene and β-caryophyllene ozonolysis products and mixtures with commercially available organic compounds [PDF]

Atmospheric Chemistry and Physics, 2020
Liquid–liquid phase separation (LLPS) in organic aerosol particles can impact several properties of atmospheric particulate matter, such as cloud condensation nuclei (CCN) properties, optical properties, and gas-to-particle partitioning.
Y.-C. Song, A. G. Bé, S. T. Martin, F. M. Geiger, A. K. Bertram, R. J. Thomson, M. Song   +6 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance

Aging and Cancer, EarlyView.
NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang, Zhong Fan, Runmeng Liu, Xiaotian Zhang   +3 more
wiley   +1 more source

Intracellular mRNA phase separation induced by cationic polymers for tumor immunotherapy

Journal of Nanobiotechnology, 2022
The formation of biomolecular condensates via liquid‒liquid phase separation (LLPS) is an advantageous strategy for cells to organize their subcellular compartments for diverse functions.
Zhen Xing, Jing Xue, Xindian Ma, Congwei Han, Zhenzhen Wang, Shunhuang Luo, Chunming Wang, Lei Dong, Junfeng Zhang   +8 more
doaj   +1 more source

Solution‐Processed Two‐Dimensional Indium Oxide on Sodium‐Embedded Alumina for Reconfigurable Optoelectronic Synaptic Transistors

Advanced Functional Materials, EarlyView.
Wafer‐scale two‐dimensioanl In2Se3 oxidized into InOx on sodium‐embedded beta‐alumina enables multifunctional reconfigurable electronics. Sodium ions accumulate within distinct spatial distribution under drain‐controlle and gate‐controlled operation. Drain‐control operation gives controllability of ultraviolet‐driven optoelectronic synaptic conductance
Jinhong Min, Yonghyun Albert Kwon, Hyunbin Kim, Jihyun Kim, Myeongjin Jung, Jaewon Heo, Gwang Ya Kim, Vlastimil Mazanek, Zdeněk Sofer, In Soo Kim, Jeong Ho Cho, Hyesung Park, Sang Min Won, Joohoon Kang   +13 more
wiley   +1 more source

Spatiotemporal deciphering of dynamic the FUS interactome during liquid-liquid phase separation in living cells

Nature Communications
Liquid-liquid phase separations (LLPS) are membraneless organelles driven by biomolecule assembly and are implicated in cellular physiological activities.
Sunfengda Song, Haiyang Xie, Qingwen Wang, Xinyi Sun, Jiasu Xu, Rui Chen, Yuankang Zhu, Lai Jiang, Xianting Ding   +8 more
doaj   +1 more source

PRC2.1 Coordinates Peri‐Nucleolar H3K27me3‐Enriched Heterochromatin Organization and NPM1 Pentamerization to Maintain Nucleolar Integrity

Advanced Science, EarlyView.
PRC2.1(PCL2)‐coordinated H3K27me3‐enriched PNH establishes a spatial scaffold crucial for nucleolar integrity. As a crucial coordinator, PCL2 links PRC2.1 to chromatin organization and NPM1 assembly. This network‐based model reveals how chromatin modifications and nucleolar components cooperatively maintain nucleolar architecture, revealing novel ...
Lina Zhu, Wenqin Wang, Shuyun Chen, Gangyi Zhu, Zhen Wu, Yue Gu, Jingwen Xiong, Xu Zhang, Heng Liu, Kyoichi Isono, Youming Zhang, Jingjing Cao, Xiangzhi Li   +12 more
wiley   +1 more source