Results 41 to 50 of about 712,786 (306)

Production of colchicine by using plant cell culture [PDF]

, 2005
Plant cell and tissue culture is an established alternative to the harvest and extraction of whole plant material for the production of valuable secondary metabolites.
Aroud, Gamal
core  

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Use of Liquid Culture with the ElecTIS Bioreactor for Faster Recovery of Blackberry (Rubus fruticosus L.) Shoots from Conservation at 4 °C

Horticulturae, 2023
ElecTIS is a new single container bioreactor which does not require forced air blowing, instead making the culture material mobile and the liquid medium stationary.
Doaa Elazab, Maurizio Capuana, Elif Aylin Ozudogru, Monica Anichini, Maurizio Lambardi   +4 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Liquid-like Solids Support Cells in 3D

, 2016
The demands of tissue engineering have driven a tremendous amount of research effort in 3D tissue culture technology and, more recently, in 3D printing.
Steve Ghivizzani, Glyn D. Palmer (569827), Glyn D. Palmer, C. Parker Gibbs (2828201), Steve Ghivizzani (2828192), Christopher S. O’Bryan, Juan M. Urueña, Tapomoy Bhattacharjee (2828186), Tapomoy Bhattacharjee, Samantha L. Marshall (2828195), Matt Carstens, W. Gregory Sawyer (2828204), C. Parker Gibbs, Benjamin Keselowsky (2828177), Benjamin Keselowsky, Carmen J. Gil, Samantha L. Marshall, Juan M. Urueña (2828189), Matt Carstens (2828207), W. Gregory Sawyer, Thomas E. Angelini, Carmen J. Gil (2828183), Thomas E. Angelini (2828198), Christopher S. O’Bryan (2828180)   +23 more
core   +2 more sources

Effect of Hormone Types and Concentrations on In Vitro Multiplication and Growth of Pineapple (Ananas Comosus (L.) Merr.) Cv Moris on Liquid MS Medium

مجلة المختار للعلوم, 2017
The effect of two cytokinins, 6-benzyleaminopurine (BAP) and kinetin (KN) and two auxins, naphthalene acetic acid (NAA) and indole acetic acid (IAA) each applied at 10 concentrations with an increment of 0.5 mg/l in the in vitro shoot formation of Moris
Abdelhamid M. Hamad
doaj   +1 more source

Data_Sheet_1_An impact of age on respiratory syncytial virus infection in air-liquid-interface culture bronchial epithelium.pdf

, 2023
BackgroundElderly people are known to be vulnerable to virus infection. However, this has not been appropriately tested in in vitro studies due to a lack of appropriate virus infection models.
Matthew Coates (6329051), Kazuhiro Ito (149202), Leah Daly (14786584)   +2 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Carbon Assimilation in Carrot Cells in Liquid Culture [PDF]

Plant Physiology, 1986
Assimilation of carbohydrates by carrot (Daucus carota L. cv Danvers) cells in liquid culture was studied to delineate the major metabolic pathways used in transformation of external carbohydrates to UDP-glucose. The cells grown on either sucrose or glucose for several years proved equally capable of utilizing each of these sugars.
J, Kanabus, R A, Bressan, N C, Carpita
openaire   +2 more sources