Liver organoid culture methods [PDF]
Cell & Bioscience, 2023 Organoids, three-dimensional structures cultured in vitro, can recapitulate the microenvironment, complex architecture, and cellular functions of in vivo organs or tissues.
Yiqing Hu +10 more
doaj +2 more sources
Human liver organoid: modeling liver steatosis and beyond [PDF]
Cell Regeneration, 2023 Steatosis, as the early stage of nonalcoholic fatty acid disease (NAFLD), would progress into nonalcoholic steatohepatitis (NASH) and liver failure without intervention.
Jinsong Wei, Wen Zhang, Bing Zhao
doaj +2 more sources
Emerging liver organoid platforms and technologies [PDF]
Cell Regeneration, 2021 Building human organs in a dish has been a long term goal of researchers in pursue of physiologically relevant models of human disease and for replacement of worn out and diseased organs.
Do Thuy Uyen Ha Lam +3 more
doaj +2 more sources
Placenta-derived factors contribute to human iPSC-liver organoid growth [PDF]
Nature CommunicationsOrganoids derived from human induced pluripotent stem cells (hiPSC) are potentially applicable for regenerative medicine. However, the applications have been hampered by limited organoid size and function as a consequence of a lack of progenitor ...
Yoshiki Kuse +13 more
doaj +2 more sources
Frontiers in Bioengineering and Biotechnology, 2022 The liver is the most important metabolic hub of endo and xenobiotic compounds. Pre-clinical studies using rodents to evaluate the toxicity of new drugs and cosmetics may produce inconclusive results for predicting clinical outcomes in humans, moreover ...
Kayque Alves Telles-Silva +7 more
doaj +3 more sources
Adult and iPS-derived non-parenchymal cells regulate liver organoid development through differential modulation of Wnt and TGF-β [PDF]
Stem Cell Research & Therapy, 2019 Background Liver organoid technology holds great promises to be used in large-scale population-based drug screening and in future regenerative medicine strategies.
Ernesto Goulart +12 more
doaj +2 more sources
A robust mouse liver organoid platform enables sustained multicellular maturation and fibrosis modeling from a single tissue sample [PDF]
Scientific ReportsEfficient isolation and culture of liver organoids are critical for studying liver fibrosis, liver regeneration and drug toxicity and screening. However, preserving mature hepatobiliary characteristics and concurrently incorporating fibrosis-producing ...
Yingyu Liang +5 more
doaj +2 more sources
Construction of mouse liver aging model by hepatocyte organoid [PDF]
Jichu yixue yu linchuang, 2023 Objective Construction of mouse liver senescence model induced rapidly in vitro by 3D organoid culture. Methods Mouse hepatocytes were collected by two-step perfusion method and cultured in 3D.
LU Yan, ZHOU Yabo, CHEN Jie
doaj +1 more source
Bioengineering Liver Organoids for Diseases Modelling and Transplantation
Bioengineering, 2022 Organoids as three-dimension (3D) cellular organizations partially mimic the physiological functions and micro-architecture of native tissues and organs, holding great potential for clinical applications.
Junzhi Li +5 more
doaj +1 more source
Investigation of Clinical Safety of Human iPS Cell-Derived Liver Organoid Transplantation to Infantile Patients in Porcine Model [PDF]
Cell Transplantation, 2020 Soichiro Murata +2 more
exaly +2 more sources