Results 141 to 150 of about 37,721 (200)

Improvement of a Streptavidin-Binding Aptamer by LNA- and α-l-LNA-Substitutions

Bioorganic and Medicinal Chemistry Letters, 2014
Forty modified versions of a streptavidin-binding aptamer each containing single or multiple LNA or α-l-LNA-substitutions were synthesized and their dissociation constants determined by surface plasmon resonance experiments. Both full-length and truncated versions of the aptamer were studied and compared with the unmodified DNA aptamers.
Jesper Wengel, Birte Vester
exaly   +5 more sources

Aptamers as a Model for Functional Evaluation of LNA and 2′-Amino LNA

Bioorganic and Medicinal Chemistry Letters, 2009
The affinity change upon incorporation of LNA and 2'-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer.
Frank J Hernandez, Jesper Wengel, Birte Vester   +2 more
exaly   +4 more sources

LNA and α-L-LNA: Towards Therapeutic Applications

Nucleosides, Nucleotides and Nucleic Acids, 2003
LNA and alpha-L-LNA are promising candidates for the development of efficient oligonucleotide-based therapeutic agents. Here, we report dose-dependent inhibition of HIV-1 Tat-dependent trans activation by a 12-mer chimeric alpha-L-LNA/DNA oligomer. This oligomer exhibits a dose-dependency similar to that of the corresponding 12-mer chimeric LNA/2'-O-Me-
Wengel, J., Vester, B., Lundberg, L.B., Douthwaite, S., Sørensen, M.D., Babu, B.R., Gait, M.J., Arzumanov, A., Petersen, M., Nielsen, J.T.   +9 more
core   +4 more sources

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Nuclease Stability of LNA Oligonucleotides and LNA-DNA Chimeras

Nucleosides, Nucleotides and Nucleic Acids, 2003
AbstractFor Abstract see ChemInform Abstract in Full Text.
Troels Koch, Henrik F Hansen
exaly   +3 more sources

Structural Characterization of LNA and α-L-LNA Hybridized to RNA

Nucleosides, Nucleotides and Nucleic Acids, 2003
LNA and alpha-L-LNA are promising candidates for the development of efficient oligonucleotide-based therapeutic agents. Here, we present a short overview of the structural results we have obtained for LNA:RNA and alpha-L-LNA:RNA hybrids. Specifically, we have shown that LNA acts as an A-type mimic, while alpha-L-LNA acts as a B-type mimic when built ...
Petersen, M.; id_orcid 0000-0002-0997-7657, Nielsen, J.T., Bondensgaard, K., Wengel, J., Jacobsen, J.P.   +4 more
openaire   +4 more sources

Lna (Locked Nucleic Acid)

Nucleosides and Nucleotides, 1999
Abstract LNA (Locked Nucleic Acid) forms duplexes with complementary DNA, RNA or LNA with unprecedented thermal affinities. CD spectra show that duplexes involving fully modified LNA (especially LNA:RNA) structurally resemble an A-form RNA:RNA duplex. NMR examination of an LNA:DNA duplex confirm the 3′-endo conformation of an LNA monomer.
Wengel, J., Koshkin, A., Singh, S. K., Nielsen, P., Meldgaard, M., Rajwanshi Singh, V. K., Kumar, R., Skouv, J., Nielsen, C. B., Jacobsen, J. P., Jacobsen, N., Olsen, C. E.   +11 more
openaire   +3 more sources

Improved Synthesis of 2′‐Amino‐LNA

ChemInform, 2003
AbstractFor Abstract see ChemInform Abstract in Full Text.
Christensen, S.M., Rosenbohm, C., Sørensen, M.., Larsen, L.-E., Wengel, J., Koch, T.   +5 more
openaire   +4 more sources

The first analogues of LNA (Locked Nucleic Acids): Phosphorothioate-LNA and 2′-thio-LNA

Bioorganic & Medicinal Chemistry Letters, 1998
LNA (Locked Nucleic Acids, 1, X = O, Y = O) is a novel oligonucleotide analogue capable of recognizing complementary DNA and RNA with unprecedented thermal affinities. Synthesis of the first chemically modified LNA analogues is reported. A 9-mer phosphorothioate-LNA containing three LNA thymine monomers (1, X = O, Y = S, Base = thymin-1-yl) and 9-mer ...
Kumar, Ravindra, Singh, Sanjay Kumar, Koshkin, Alexei, Rajwanshi, Vivek Kumar, Meldgaard, Michael, Wengel, Jesper Thagaard   +5 more
openaire   +3 more sources