Results 141 to 150 of about 23,272 (196)

Improvement of a streptavidin-binding aptamer by LNA- and α-l-LNA-substitutions

Bioorganic and Medicinal Chemistry Letters, 2014
Forty modified versions of a streptavidin-binding aptamer each containing single or multiple LNA or α-l-LNA-substitutions were synthesized and their dissociation constants determined by surface plasmon resonance experiments. Both full-length and truncated versions of the aptamer were studied and compared with the unmodified DNA aptamers.
Birte Vester, Jesper Wengel
exaly   +4 more sources

LNA and α-L-LNA: Towards Therapeutic Applications

Nucleosides, Nucleotides and Nucleic Acids, 2003
LNA and alpha-L-LNA are promising candidates for the development of efficient oligonucleotide-based therapeutic agents. Here, we report dose-dependent inhibition of HIV-1 Tat-dependent trans activation by a 12-mer chimeric alpha-L-LNA/DNA oligomer. This oligomer exhibits a dose-dependency similar to that of the corresponding 12-mer chimeric LNA/2'-O-Me-
Wengel, J., Vester, B., Lundberg, L.B., Douthwaite, S., Sørensen, M.D., Babu, B.R., Gait, M.J., Arzumanov, A., Petersen, M., Nielsen, J.T.   +9 more
openaire   +3 more sources

The first analogues of LNA (Locked Nucleic Acids): Phosphorothioate-LNA and 2′-thio-LNA

Bioorganic & Medicinal Chemistry Letters, 1998
LNA (Locked Nucleic Acids, 1, X = O, Y = O) is a novel oligonucleotide analogue capable of recognizing complementary DNA and RNA with unprecedented thermal affinities. Synthesis of the first chemically modified LNA analogues is reported. A 9-mer phosphorothioate-LNA containing three LNA thymine monomers (1, X = O, Y = S, Base = thymin-1-yl) and 9-mer ...
Kumar, Ravindra, Singh, Sanjay Kumar, Koshkin, Alexei, Rajwanshi, Vivek Kumar, Meldgaard, Michael, Wengel, Jesper Thagaard   +5 more
openaire   +3 more sources

LNA characterization methodologies

International Journal of Circuit Theory and Applications, 2016
SummaryJoint characterization of low‐noise amplifiers regarding stochastic and deterministic parameters based on output power measurement for a set of known input termination impedances is considered. As an advantage of this method, both noise parameters and the input impedance can be obtained for narrow and broad band applications without special ...
Bernhard Lehmeyer, Michel T. Ivrlac, Josef A. Nossek   +2 more
openaire   +1 more source

Structural Characterization of LNA and α-L-LNA Hybridized to RNA

Nucleosides, Nucleotides and Nucleic Acids, 2003
LNA and alpha-L-LNA are promising candidates for the development of efficient oligonucleotide-based therapeutic agents. Here, we present a short overview of the structural results we have obtained for LNA:RNA and alpha-L-LNA:RNA hybrids. Specifically, we have shown that LNA acts as an A-type mimic, while alpha-L-LNA acts as a B-type mimic when built ...
Petersen, M.; id_orcid 0000-0002-0997-7657, Nielsen, J.T., Bondensgaard, K., Wengel, J., Jacobsen, J.P.   +4 more
openaire   +3 more sources

STRUCTURAL STUDIES ON LNA QUADRUPLEXES

Nucleosides, Nucleotides & Nucleic Acids, 2005
LNAs (locked nucleic acids) are new DNA analogues with higher binding affinities toward nucleic acids than the canonical counterparts mainly due to the characteristic conformational restriction arising from the 2'-O, 4'-C methylene bridge. In light of the promising therapeutic applications and considering the advantageous characteristics of LNAs, such ...
RANDAZZO, ANTONIO, ESPOSITO, VERONICA, OLIVER OHLENSCHLÄGER, RAMADURAI RAMACHANDRAN, VIRGILIO, ANTONELLA, MAYOL, LUCIANO   +5 more
openaire   +3 more sources

FISHing with locked nucleic acids (LNA): evaluation of different LNA/DNA mixmers

Molecular and Cellular Probes, 2003
Locked Nucleic Acids (LNA) constitute a novel class of DNA analogues that have an exceptionally high affinity towards complementary DNA and RNA. Using human classical satellite-2 repeat sequence clusters as targets, we demonstrate that LNA/DNA mixmers oligonucleotides are excellent probes for FISH combining high binding affinity with short ...
Asli Silahtaroĝlu, Niels Tommerup
exaly   +3 more sources

In Vitro Incorporation of LNA Nucleotides

Nucleosides, Nucleotides & Nucleic Acids, 2007
An LNA modified nucleoside triphosphate 1 was synthesized in order to investigate its potential to act as substrate for DNA strand synthesis by polymerases. Primer extension assays for the incorporation experiments revealed that Phusion High Fidelity DNA polymerase is an efficient enzyme for incorporation of the LNA nucleotide and for extending strand ...
Veedu, R.N., Vester, B., Wengel, J.
openaire   +5 more sources

High-affinity nucleic acid recognition using ‘LNA’ (locked nucleic acid, β-D-ribo configured LNA), ‘xylo-LNA’ (β-D-xylo configured LNA) or ‘α-L-LNA’ (α-L-ribo configured LNA)

Chemical Communications, 1999
Remarkably strong binding affinities towards complementary single stranded DNA and RNA were obtained for 10- and 14-mer LNAs (locked nucleic acids) containing diastereoisomeric β-D-ribo, β-D-xylo or α-L-ribo configured LNA thymine monomers; a possible relevance of these results in relation to nucleic acid evolution is discussed.
Rajwanshi, Vivek Kumar, Håkansson, Anders Eckart, Kumar, Ravindra, Wengel, Jesper Thagaard   +3 more
openaire   +1 more source

SiGe LNAs for UMTS System

Analog Integrated Circuits and Signal Processing, 2001
Three LNAs at 2 GHz frequency range have been implemented in a SiGe Bipolar process targeted for a universal mobile telecommunications system. The LNAs are operating in two gain modes and they include a power-down mode. Both on-wafer and packaged LNAs were measured.
Ryynänen Jussi, Halonen Kari, Stadius Kari   +2 more
openaire   +1 more source