Results 41 to 50 of about 37,721 (200)

Nuclease resistant methylphosphonate-DNA/LNA chimeric oligonucleotides [PDF]

, 2009
Synthesis of chimeric 9-mer oligonucleotides containing methylphosphonate-linkages and locked nucleic acid (LNA) monomers, their binding affinity towards complementary DNA and RNA, and their 3′-exonucleolytic stability are described.
Jesper Wengel, Veedu, Rakesh N., Nagahama, K., Rakesh N. Veedu, Nagahama, Koji, Koji Nagahama, Veedu, R.N., Wengel, Jesper, Wengel, J.   +8 more
core   +1 more source

A Sensitive In Vitro Approach to Assess the Hybridization-Dependent Toxic Potential of High Affinity Gapmer Oligonucleotides

Molecular Therapy: Nucleic Acids, 2018
The successful development of high-affinity gapmer antisense oligonucleotide (ASO) therapeutics containing locked nucleic acid (LNA) or constrained ethyl (cEt) substitutions has been hampered by the risk of hepatotoxicity.
Andreas Dieckmann, Peter H. Hagedorn, Yvonne Burki, Christine Brügmann, Marco Berrera, Martin Ebeling, Thomas Singer, Franz Schuler   +7 more
doaj   +1 more source

Efficient reverse transcription using locked nucleic acid nucleotides towards the evolution of nuclease resistant RNA aptamers. [PDF]

PLoS ONE, 2012
BACKGROUND: Modified nucleotides are increasingly being utilized in the de novo selection of aptamers for enhancing their drug-like character and abolishing the need for time consuming trial-and-error based post-selection modifications.
Lucile Crouzier, Camille Dubois, Stacey L Edwards, Lasse H Lauridsen, Jesper Wengel, Rakesh N Veedu   +5 more
doaj   +1 more source

Lateral Nasal Artery Perforator Flaps: Anatomic Study and Clinical Applications

Archives of Plastic Surgery, 2016
Background Previous studies have investigated facial artery perforators, but have reported inconsistent results regarding lateral nasal artery (LNA) perforators.
Giuseppe AG Lombardo, Serena Tamburino, Luciano Tracia, Maria Stella Tarico, Rosario Emanuele Perrotta   +4 more
doaj   +1 more source

Projeto e caracterização de amplificadores de baixo ruído em 2,4 ghz [PDF]

, 2012
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia ElétricaEsta dissertação está centrada em amplificadores de baixo ruído (LNAs) e pode ser dividida em três partes.
Silva, Paulo Márcio Moreira e
core  

Polymerase directed incorporation studies of LNA-G nucleoside 5'-triphosphate and primer extension involving all four LNA nucleotides

, 2010
For the first time, the enzymatic incorporation of LNA-Gnucleotides into DNA strands has been investigated. KODDNA polymerase accepted LNA-GTP as a substrate and provided a full-length dsDNA product modified with LNA-Gnucleotides.
Veedu, Rakesh N., Vester, B., Veedu, R.N., Wengel, Jesper, Wengel, J., Vester, Birte, Jesper Wengel, Rakesh N. Veedu, Birte Vester   +8 more
core   +1 more source

Spatio-Temporal Variations of High and Low Nucleic Acid Content Bacteria in an Exorheic River. [PDF]

PLoS ONE, 2016
Bacteria with high nucleic acid (HNA) and low nucleic acid (LNA) content are commonly observed in aquatic environments. To date, limited knowledge is available on their temporal and spatial variations in freshwater environments.
Jie Liu, Zhenyu Hao, Lili Ma, Yurui Ji, Mark Bartlam, Yingying Wang   +5 more
doaj   +1 more source

Enzymatic polymerisation involving 2'-amino-LNA nucleotides

, 2012
The triphosphate of the thymine derivative of 2′-amino-LNA (2′-amino-LNA-TTP) was synthesised and found to be a good substrate for Phusion® HF DNA polymerase, allowing enzymatic synthesis of modified DNA encoded by an unmodified template.
Veedu, Rakesh N, Madsen, Andreas Stahl, Johannsen, Marie W, Veedu, Rakesh N., Madsen, A.S., Johannsen, M.W., Johannsen, Marie W., Veedu, R.N., Wengel, Jesper, Wengel, J.   +9 more
core   +1 more source

LNA (locked nucleic acid) and the diastereoisomeric (alfa)-L-LNA: Conformational tuning and high-affinity recognition of DNA/RNA targets

, 2001
The remarkable binding properties of LNA (Locked Nucleic Acid) and α-L-LNA (the α-L-ribo configured diastereoisomer of LNA) are summarized, and hybridization results for LNA/2′-O-Me-RNA chimera and LNAs with a “dangling” nucleotide are introduced.
Håkansson, A.E., Bryld, T., Petersen, M.; id_orcid, Nielsen, K.E., Rajwanshi, V.K., Kumar, R., Jacobsen, J.P., Sørensen, M.D., Wengel, J., Jensen, G.A.   +9 more
core   +1 more source

Synthesis of 2'-amino-LNA: A new strategy [PDF]

, 2003
In this paper we present revised and significantly improved synthetic routes to 2’-amino-LNA (locked nucleic acid). The optimal route is convergent with the synthesis of LNA monomers (“2’-oxy-LNA”)via a common intermediate obtained by a mild ...
Pedersen, Daniel Sejer, Sørensen, Mads Detlef, Larsen, Lotte-Emilie, Koch, Troels, Wengel, Jesper, Christensen, S.M., Rosenbohm, Christoph, Pedersen, D.S., Christensen, Signe M., Sorensen, Mads D., Sørensen, M.D., Larsen, L.-E., Wengel, J., Koch, T., Rosenbohm, C.   +14 more
core   +1 more source