Results 41 to 50 of about 23,328 (215)

Introduction of a Double‐Headed Nucleotide Into G‐Quadruplex DNA: Position‐Dependent Stabilization and Structural Insights

Chemistry – A European Journal, EarlyView.
A double‐headed bis‐guanine (G) nucleotide (GG) was evaluated as a noncanonical building block in G‐quadruplexes (G4s). Biophysical and computational analyses reveal that GG can directly participate in G‐tetrads and strongly stabilize G4s in a position‐ and topology‐dependent manner. These findings establish design rules for using GG to engineer highly
Krista Urup, Peter Reinholdt, Kasper Munch Beck, Michael Petersen, Jean‐Louis Mergny, Poul Nielsen   +5 more
wiley   +1 more source

Epilepsy‐associated SCN2A‐L1342P mutation drives network hyperexcitability and widespread transcriptomic changes in human cortical organoids

Epilepsia, EarlyView.
Abstract Objective SCN2A pathogenic mutations, such as the recurrent heterozygous Nav1.2‐L1342P, are monogenic causes of epilepsy. In this human‐induced pluripotent stem cell–derived model system, we aim to investigate the molecular and cellular mechanisms underlying SCN2A‐L1342P‐associated pathology. Methods Using a human male induced pluripotent stem
Maria I. Olivero‐Acosta, Morgan Robinson, Zhefu Que, Zaiyang Zhang, Karen V. Salazar‐Salazar, Hope Elizabeth Harlow, Vinayak Shankar, Seoyeon Hong, Muhan Wang, Conrad M. Otterbacher, Hina Kadono, Manasi Halurkar, Harish Kothandaraman, Nadia Atallah Lanman, Trang Nguyen, Kyle Wettschurack, Benjamin Zirkle, Layan Yunis, Ningren Cui, Xiaoling Chen, Jingliang Zhang, Jiaxiang Wu, William C. Skarnes, Chongli Yuan, Feng Guo, Megan Abbott, Yang Yang   +26 more
wiley   +1 more source

Preparation and Study of Solid Lipid Nanoparticles Based on Curcumin, Resveratrol and Capsaicin Containing Linolenic Acid

Pharmaceutics, 2022
Linolenic acid (LNA) is the most highly consumed polyunsaturated fatty acid found in the human diet. It possesses anti-inflammatory effects and the ability to reverse skin-related disorders related to its deficiency.
Roberta Cassano, Simona Serini, Federica Curcio, Sonia Trombino, Gabriella Calviello   +4 more
doaj   +1 more source

The plant microbiome: From ecological foundations to precision microbial engineering for sustainable agriculture

iMeta, EarlyView.
From ecological principles to precision engineering of plant microbiomes for sustainable agriculture. The plant holobiont integrates diverse microbial niches (including rhizosphere, endosphere, phyllosphere, seed, and aerial‐root mucilagesphere), host genetics (namely M genes), and host metabolites. Furthermore, molecular dialogues, including rhizobial
Mi Wei, Tengxiang Lian, Liying Chen, Lanxiang Wang, Junjie Ye, Xiaofang Yao, Shilong Duan, Ziyu Lu, Jinji Tu, Hongfu Li, Xin‐Yue Xu, Jie Zhou, Junliang He, Feiying Zhu, Paola Bonfante, Lam‐Son Phan Tran, Zhiqiang Pang, Xin Zhou, Zhihui Xu, Lin Zhang, Mengcen Wang, Xiaolin Wang, Chang‐Fu Tian, Yong‐Xin Liu, Kai Sun, Ertao Wang, Xianan Xie   +26 more
wiley   +1 more source

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Straw Return Combined With Nitrogen Fertilizer Application Improve Nitrogen Utilization Efficiency by Regulating Soil Physical and Chemical Properties in Spring Maize

Land Degradation &Development, EarlyView.
ABSTRACT Straw return (SR) and controlled‐release nitrogen fertilizers application can improve soil structure, enhance soil water retention, and promote crop growth. However, the mechanisms through which SR and controlled‐release nitrogen fertilizers application jointly modulate soil physical and chemical properties remain unclear.
Min An, Fuying Hu, Xianghui Ou, Xianjie Tan, Zhenwei Li, Chengqiao Shi, Rui He, Xunbo Zhou   +7 more
wiley   +1 more source

Toward Highest Frequency, Phase‐Sensitive Electric Field Sensing Enabled by Plasmonic Circuits

Laser &Photonics Reviews, EarlyView.
An electromagnetic field sensor using an optical sensor head for distortion‐free, high‐sensitivity measurements with potential for high‐frequency operation is presented. The frequency response is enabled by an antenna‐integrated plasmonic modulator. The sensitivity is calculated, and optimizations to obtain thermal‐background‐limited sensitivity are ...
Michael Baumann, Hande Ibili, Boris Vukovic, Tobias Blatter, Jasmin Smajic, Juerg Leuthold   +5 more
wiley   +1 more source

A Ku band high performance low-cost low noise amplifier

Dianzi Jishu Yingyong
Based on unmatched plastic-package GaAs die and SMT (Surface Mounted Technology) hybrid integration process, a low-cost Ku band 2-stage LNA (Low Noise Amplifier) was realized on single-layer double-side high-frequency PCB (Printed Circuit Board).
Zhang Kai, Liu Shuai, Zhao Xiaodong
doaj   +1 more source

Image Encoding With Blipped Gradients in an Echo Train

Magnetic Resonance in Medicine, EarlyView.
ABSTRACT Purpose An MRI scanner was designed and built to encode k$$ k $$‐space points in the spin echoes occurring between 180∘$$ 18{0}^{\circ } $$ pulses in an echo train by using blipped B0$$ {B}_0 $$ gradient pulses applied just prior to those spin echoes.
Logi Vidarsson, Gordon E. Sarty
wiley   +1 more source

Pharmacokinetics and Pharmacodynamics of a 13-mer LNA-inhibitor-miR-221 in Mice and Non-human Primates

Molecular Therapy: Nucleic Acids, 2016
Locked nucleic acid (LNA) oligonucleotides have been successfully used to efficiently inhibit endogenous small noncoding RNAs in vitro and in vivo. We previously demonstrated that the direct miR-221 inhibition by the novel 13-mer LNA-i-miR-221 induces ...
Maria Eugenia Gallo Cantafio, Boye Schnack Nielsen, Chiara Mignogna, Mariamena Arbitrio, Cirino Botta, Niels M Frandsen, Christian Rolfo, Pierosandro Tagliaferri, Pierfrancesco Tassone, Maria Teresa Di Martino   +9 more
doaj   +1 more source