Results 1 to 10 of about 4,296 (70)

DNA read count calibration for single-molecule, long-read sequencing

Scientific Reports, 2022
There are many applications in which quantitative information about DNA mixtures with different molecular lengths is important. Gene therapy vectors are much longer than can be sequenced individually via short-read NGS.
Luis M. M. Soares, Terrence Hanscom, Donald E. Selby, Samuel Adjei, Wei Wang, Dariusz Przybylski, John F. Thompson   +6 more
doaj   +1 more source

Long-read sequencing in fungal identification [PDF]

Microbiology Australia, 2022
Long-read sequencing is currently supported by sequencing platforms from Pacific Biosciences and Oxford Nanopore Technologies, both of which generate ultra-long reads.
Wieland Meyer, Laszlo Irinyi, Minh Thuy Vi Hoang   +2 more
doaj  

Are we there yet? Benchmarking low-coverage nanopore long-read sequencing for the assembling of mitochondrial genomes using the vulnerable silky shark Carcharhinus falciformis

BMC Genomics, 2022
Background Whole mitochondrial genomes are quickly becoming markers of choice for the exploration of within-species genealogical and among-species phylogenetic relationships. Most often, ‘primer walking’ or ‘long PCR’ strategies plus Sanger sequencing or
J. Antonio Baeza, F. J. García-De León
doaj   +1 more source

Ultra-accurate microbial amplicon sequencing with synthetic long reads

Microbiome, 2021
Background Out of the many pathogenic bacterial species that are known, only a fraction are readily identifiable directly from a complex microbial community using standard next generation DNA sequencing.
Benjamin J. Callahan, Dmitry Grinevich, Siddhartha Thakur, Michael A. Balamotis, Tuval Ben Yehezkel   +4 more
doaj   +1 more source

Will long-read sequencing technologies replace short-read sequencing technologies in the next 10 years?

African Journal of Laboratory Medicine, 2020
No abstract available.
Boluwatife A. Adewale
doaj   +1 more source

Comparing genomes recovered from time-series metagenomes using long- and short-read sequencing technologies

Microbiome, 2023
Background Over the past years, sequencing technologies have expanded our ability to examine novel microbial metabolisms and diversity previously obscured by isolation approaches.
Luis H. Orellana, Karen Krüger, Chandni Sidhu, Rudolf Amann   +3 more
doaj   +1 more source

Long-read sequencing settings for efficient structural variation detection based on comprehensive evaluation

BMC Bioinformatics, 2021
Background With the rapid development of long-read sequencing technologies, it is possible to reveal the full spectrum of genetic structural variation (SV).
Tao Jiang, Shiqi Liu, Shuqi Cao, Yadong Liu, Zhe Cui, Yadong Wang, Hongzhe Guo   +6 more
doaj   +1 more source

Long-Read Sequencing Improves Recovery of Picoeukaryotic Genomes and Zooplankton Marker Genes from Marine Metagenomes

mSystems, 2022
Long-read sequencing offers the potential to improve metagenome assemblies and provide more robust assessments of microbial community composition and function than short-read sequencing.
N. V. Patin, K. D. Goodwin
doaj   +1 more source

Balancing read length and sequencing depth: Optimizing Nanopore long‐read sequencing for monocots with an emphasis on the Liliales

Applications in Plant Sciences, 2023
Premise We present approaches used to generate long‐read Nanopore sequencing reads for the Liliales and demonstrate how modifications to standard protocols directly impact read length and total output.
Gisel Y. De La Cerda, Jacob B. Landis, Evan Eifler, Adriana I. Hernandez, Fay‐Wei Li, Jing Zhang, Carrie M. Tribble, Nisa Karimi, Patricia Chan, Thomas Givnish, Susan R. Strickler, Chelsea D. Specht   +11 more
doaj   +1 more source

Long‐fragment targeted capture for long‐read sequencing of plastomes

Applications in Plant Sciences, 2019
Premise Third‐generation sequencing methods generate significantly longer reads than those produced using alternative sequencing methods. This provides increased possibilities for the study of biodiversity, phylogeography, and population genetics.
Kevin Bethune, Cédric Mariac, Marie Couderc, Nora Scarcelli, Sylvain Santoni, Morgane Ardisson, Jean‐François Martin, Rommel Montúfar, Valentin Klein, François Sabot, Yves Vigouroux, Thomas L. P. Couvreur   +11 more
doaj   +1 more source