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Profiling the epigenome using long-read sequencing
Nature GeneticsThe advent of single-molecule, long-read sequencing (LRS) technologies by Oxford Nanopore Technologies and Pacific Biosciences has revolutionized genomics, transcriptomics and, more recently, epigenomics research. These technologies offer distinct advantages, including the direct detection of methylated DNA and simultaneous assessment of DNA sequences ...
Tianyuan Liu, Ana Conesa
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Long-read sequencing for rare human genetic diseases
Journal of Human Genetics, 2019During the past decade, the search for pathogenic mutations in rare human genetic diseases has involved huge efforts to sequence coding regions, or the entire genome, using massively parallel short-read sequencers. However, the approximate current diagnostic rate is
Satomi, Mitsuhashi, Naomichi, Matsumoto
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Efficient short-read sequencing on long-read sequencers
2020We present SMURF-seq, a protocol to efficiently sequence short DNA molecules on a long-read sequencer by randomly ligating them to form long molecules. Applying SMURF-seq using the highly portable and inexpensive Oxford Nanopore MinION yields up to 30 fragments per read, providing an average of 6.2 and up to 7.5 million mappable fragments per run ...
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Long-read Sequencing Technology
This 50 minute lecture on long-read sequencing technology covers the following items: 1) Review of RNA-Seq Short-read Sequencing, 2) Overview and benefits of Long-read Sequencing, 3) Oxford Nanopore Sequencing, and 4) Pacific Biosciences Sequencing.openaire +1 more source
Genome sequencing: Long reads for a short plant
Nature Plants, 2015The genome of a tiny resurrection plant has been sequenced using PacBio's long-read single-molecule real-time sequencing technology, aiding the understanding of extreme desiccation tolerance. The genome contiguity is comparable to that of genomes sequenced using far more laborious approaches.
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Ottelia alismoides long-read RNA sequenceing
This project sequenced Ottelia alismoides leaves RNA sequences under high and low CO2 treatments at dark and light conditions. Total RNA from two different treatments at two different times were mixed together for sequencing. There long-read sequences were used to provide gene sequences.openaire +1 more source
Long‐Read Sequencing Could Increase Diagnosis Rates
American Journal of Medical Genetics Part A, 2021openaire +2 more sources
Transcriptome variation in human tissues revealed by long-read sequencing
Nature, 2022Dafni A Glinos +2 more
exaly
Iso-Seq Long Read Transcriptome Sequencing
2021Nam V. Hoang, Robert J. Henry
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