Results 261 to 270 of about 7,946,195 (290)

Transcript Identification Through Long-Read Sequencing

2021
RNA-seq using long-read sequencing, such as nanopore and SMRT (Single Molecule, Real-Time) sequencing, enabled the identification of the full-length structure of RNA molecules. Several tools for long-read RNA-seq were developed recently. In this section, we introduce an analytical pipeline of long-read RNA-seq for isoform identification and the ...
Masahide, Seki, Miho, Oka, Liu, Xu, Ayako, Suzuki, Yutaka, Suzuki   +4 more
openaire   +2 more sources

Long-read sequencing of new Drosophila genomes

Nature Reviews Genetics, 2021
Kim et al. present a drosophilid genome resource comprising 101 de novo genome assemblies from 93 drosophilid species obtained by nanopore sequencing.
openaire   +2 more sources

Assembling Plant Genomes with Long-Read Sequencing

2022
Continuous improvements in long-read sequencing allow us to tackle increasingly big and complex genomes. Here we present the principles of long-read genome assembly, taking Solanum pennellii nanopore sequencing as an example.
openaire   +2 more sources

Contributions of Long-Read Sequencing for the Detection of Antimicrobial Resistance

Pathogens
Background. In the context of increasing antimicrobial resistance (AMR), whole-genome sequencing (WGS) of bacteria is considered a highly accurate and comprehensive surveillance method for detecting and tracking the spread of resistant pathogens.
Stéphane Emonet, Julien Prados, Roberto Sierra   +2 more
exaly   +5 more sources

Profiling the epigenome using long-read sequencing

Nature Genetics
The advent of single-molecule, long-read sequencing (LRS) technologies by Oxford Nanopore Technologies and Pacific Biosciences has revolutionized genomics, transcriptomics and, more recently, epigenomics research. These technologies offer distinct advantages, including the direct detection of methylated DNA and simultaneous assessment of DNA sequences ...
Tianyuan Liu, Ana Conesa
openaire   +3 more sources

Method of the year: long-read sequencing

Nature Methods, 2023
openaire   +2 more sources

Genome sequencing: Long reads for a short plant

Nature Plants, 2015
The genome of a tiny resurrection plant has been sequenced using PacBio's long-read single-molecule real-time sequencing technology, aiding the understanding of extreme desiccation tolerance. The genome contiguity is comparable to that of genomes sequenced using far more laborious approaches.
openaire   +2 more sources

Long-read Sequencing Technology

This 50 minute lecture on long-read sequencing technology covers the following items: 1) Review of RNA-Seq Short-read Sequencing, 2) Overview and benefits of Long-read Sequencing, 3) Oxford Nanopore Sequencing, and 4) Pacific Biosciences Sequencing.
openaire   +1 more source

Methods for long read sequencing

2021
GLEZER ELI N, ABDU ABREHET, LOONEY TIMOTHY   +2 more
openaire   +3 more sources

Long-read sequencing in the era of epigenomics and epitranscriptomics

Nature Methods, 2023
Morghan C. Lucas, Eva Maria Novoa
openaire   +2 more sources