Results 261 to 270 of about 8,328,477 (294)

Amplicon Sequencing using the Long-Read Sequencing Technologies

Journal of Visualized Experiments
The World Health Organization (WHO) continues to emphasize the urgent need for a rapid, cost-effective, and user-friendly diagnostic method for tuberculosis (TB) and drug-resistant TB (DR-TB). Next-generation sequencing (NGS) technologies, endorsed by the WHO, have significantly improved the detection of DR-TB.
Morwasehla, Modjadji, Brendon Coenrad, Mann, Johannes, Loubser, Jennifer, Williams, Janré, Steyn, Elizabeth Maria, Streicher, Melanie, Grobbelaar, Robin Mark, Warren   +7 more
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Transcript Identification Through Long-Read Sequencing

2021
RNA-seq using long-read sequencing, such as nanopore and SMRT (Single Molecule, Real-Time) sequencing, enabled the identification of the full-length structure of RNA molecules. Several tools for long-read RNA-seq were developed recently. In this section, we introduce an analytical pipeline of long-read RNA-seq for isoform identification and the ...
Masahide, Seki, Miho, Oka, Liu, Xu, Ayako, Suzuki, Yutaka, Suzuki   +4 more
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Long-read sequencing of new Drosophila genomes

Nature Reviews Genetics, 2021
Kim et al. present a drosophilid genome resource comprising 101 de novo genome assemblies from 93 drosophilid species obtained by nanopore sequencing.
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Assembling Plant Genomes with Long-Read Sequencing

2022
Continuous improvements in long-read sequencing allow us to tackle increasingly big and complex genomes. Here we present the principles of long-read genome assembly, taking Solanum pennellii nanopore sequencing as an example.
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Profiling the epigenome using long-read sequencing

Nature Genetics
The advent of single-molecule, long-read sequencing (LRS) technologies by Oxford Nanopore Technologies and Pacific Biosciences has revolutionized genomics, transcriptomics and, more recently, epigenomics research. These technologies offer distinct advantages, including the direct detection of methylated DNA and simultaneous assessment of DNA sequences ...
Tianyuan Liu, Ana Conesa
openaire   +3 more sources

Method of the year: long-read sequencing

Nature Methods, 2023
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Genome sequencing: Long reads for a short plant

Nature Plants, 2015
The genome of a tiny resurrection plant has been sequenced using PacBio's long-read single-molecule real-time sequencing technology, aiding the understanding of extreme desiccation tolerance. The genome contiguity is comparable to that of genomes sequenced using far more laborious approaches.
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Long-read Sequencing Technology

This 50 minute lecture on long-read sequencing technology covers the following items: 1) Review of RNA-Seq Short-read Sequencing, 2) Overview and benefits of Long-read Sequencing, 3) Oxford Nanopore Sequencing, and 4) Pacific Biosciences Sequencing.
openaire   +1 more source

Long-read sequencing in the era of epigenomics and epitranscriptomics

Nature Methods, 2023
Morghan C. Lucas, Eva Maria Novoa
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Perspectives and Benefits of High-Throughput Long-Read Sequencing in Microbial Ecology.

Applied and Environmental Microbiology, 2021
Leho Tedersoo, Sten Anslan, Mads Albertsen   +2 more
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