Colorimetric LPMO assay with direct implication for cellulolytic activity [PDF]
Biotechnology for Biofuels, 2021 Background Lytic polysaccharide monooxygenases (LPMOs) are important industrial enzymes known for their catalytic degradation of recalcitrant polymers such as cellulose or chitin.
Søren Brander +7 more
doaj +7 more sources
A fast, sensitive and fluorescent LPMO activity assay
Frontiers in Microbiology, 2023 Lytic polysaccharide monooxygenases (LPMOs) are industrially relevant enzymes that utilize a copper co-factor and an oxygen species to break down recalcitrant polysaccharides.
Johan Ø. Ipsen +2 more
doaj +6 more sources
Oxidative Power: Tools for Assessing LPMO Activity on Cellulose [PDF]
Biomolecules, 2021 Lytic polysaccharide monooxygenases (LPMOs) have sparked a lot of research regarding their fascinating mode-of-action. Particularly, their boosting effect on top of the well-known cellulolytic enzymes in lignocellulosic hydrolysis makes them industrially
Federica Calderaro +2 more
doaj +5 more sources
LPMO direct electrochemical characterization
, 2022 C.M. Cordas, G.N. Valério, A.K. Røhr, V.G.H. Eijsink, J.J.G. Moura. (2022). LPMO direct electrochemical characterization. FEBS Open Bio, 12 (Suppl. S1): P-04.1-071.
Cordas, Cristina M. +4 more
openaire +3 more sources
Polyphenol Oxidase Products Are Priming Agents for LPMO Peroxygenase Activity
ChemSusChem, 2023 AbstractPolyphenol oxidases catalyze the hydroxylation of monophenols to diphenols, which are reducing agents for lytic polysaccharide monooxygenases (LPMOs) in their degradation of cellulose. In particular, the polyphenol oxidase MtPPO7 from Myceliophthora thermophila converts lignocellulose‐derived monophenols, and under the new perspective of the ...
de Oliveira Gorgulho Silva, Caio +5 more
openaire +3 more sources
Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters [PDF]
Biotechnology for Biofuels, 2019 Background The increasing interest in replacing petroleum-based products by more sustainable materials in the packaging sector gives relevance to cellulose as a biodegradable natural resource.
Cristina Valls +6 more
doaj +2 more sources
Enzymatic degradation of sulfite-pulped softwoods and the role of LPMOs [PDF]
Biotechnology for Biofuels, 2017 Background Recent advances in the development of enzyme cocktails for degradation of lignocellulosic biomass, especially the discovery of lytic polysaccharide monooxygenases (LPMOs), have opened new perspectives for process design and optimization ...
Piotr Chylenski +8 more
doaj +8 more sources
Inhibition of LPMOs by Fermented Persimmon Juice [PDF]
Biomolecules, 2021 Fermented persimmon juice, Kakishibu, has traditionally been used for wood and paper protection. This protective effect stems at least partially from inhibition of microbial cellulose degrading enzymes.
Radina Tokin +5 more
doaj +5 more sources
On the functional characterization of lytic polysaccharide monooxygenases (LPMOs) [PDF]
Biotechnology for Biofuels, 2019 Lytic polysaccharide monooxygenases (LPMOs) are abundant in nature and best known for their role in the enzymatic conversion of recalcitrant polysaccharides such as chitin and cellulose. LPMO activity requires an oxygen co-substrate, which was originally
Vincent G. H. Eijsink +7 more
doaj +4 more sources
Comparison of solid and liquid fractions of pretreated Norway spruce as reductants in LPMO-supported saccharification of cellulose [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 The role of lignin in enzymatic saccharification of cellulose involving lytic polysaccharide monooxygenase (LPMO) was investigated in experiments with the solid and liquid fractions of pretreated Norway spruce from a biorefinery demonstration plant using
Chaojun Tang +2 more
doaj +2 more sources