Results 21 to 30 of about 2,233 (191)

Boosting LPMO-driven lignocellulose degradation by polyphenol oxidase-activated lignin building blocks

Biotechnology for Biofuels, 2017
Background Many fungi boost the deconstruction of lignocellulosic plant biomass via oxidation using lytic polysaccharide monooxygenases (LPMOs). The application of LPMOs is expected to contribute to ecologically friendly conversion of biomass into fuels ...
Matthias Frommhagen, Sumanth Kumar Mutte, Adrie H. Westphal, Martijn J. Koetsier, Sandra W. A. Hinz, Jaap Visser, Jean-Paul Vincken, Dolf Weijers, Willem J. H. van Berkel, Harry Gruppen, Mirjam A. Kabel   +10 more
doaj   +2 more sources

LPMO-oxidized cellulose oligosaccharides evoke immunity in Arabidopsis conferring resistance towards necrotrophic fungus B. cinerea

Communications Biology, 2021
Zarattini et al. confirm the capacity of Lytic Polysaccharide Monooxygenases (LPMO) active on cellulose to trigger immune responses in Arabidopsis. These results bring insight to the field of cell wall modifying enzymes and their roles in plant defense ...
Marco Zarattini, Massimiliano Corso, Marco Antonio Kadowaki, Antonielle Monclaro, Silvia Magri, Irma Milanese, Sylvie Jolivet, Mariana Ortiz de Godoy, Christian Hermans, Mathilde Fagard, David Cannella   +10 more
doaj   +2 more sources

Enhancing enzymatic saccharification yields of cellulose at high solid loadings by combining different LPMO activities

Biotechnology for Biofuels and Bioproducts
Background The polysaccharides in lignocellulosic biomass hold potential for production of biofuels and biochemicals. However, achieving efficient conversion of this resource into fermentable sugars faces challenges, especially when operating at ...
Camilla F. Angeltveit, Anikó Várnai, Vincent G. H. Eijsink, Svein J. Horn   +3 more
doaj   +2 more sources

Lytic polysaccharide monooxygenases (LPMOs) facilitate cellulose nanofibrils production [PDF]

Biotechnology for Biofuels, 2019
Background Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that cleave polysaccharides through an oxidative mechanism. These enzymes are major contributors to the recycling of carbon in nature and are currently used in the ...
Céline Moreau, Sandra Tapin-Lingua, Sacha Grisel, Isabelle Gimbert, Sophie Le Gall, Valérie Meyer, Michel Petit-Conil, Jean-Guy Berrin, Bernard Cathala, Ana Villares   +9 more
doaj   +6 more sources

Assessing the activity of a modular AA9 LPMO on cellulosic substrates

, 2020
Évaluation de l'activité d'une LPMO AA9 modulaire sur des substrats cellulosiques Les « lytic polysaccharides monooxygenases »(LPMO), sécrétées par les champignons filamenteux, jouent un rôle important dans la dégradation de la biomasse lignocellulosique récalcitrante.
Chalak, Amani
openaire   +3 more sources

Experimental estimation of copper-site geometry reproducibility in biologically relevant redox and saccharide-bound states of a model lytic polysaccharide monooxygenase. [PDF]

Acta Crystallogr D Struct Biol
Small but measurable changes in the coordination geometry of a lytic polysaccharide monooxygenase copper active site are revealed by X‐ray crystallography, providing structural insight into their catalytic flexibility.Lytic polysaccharide monooxygenases (LPMOs) are copper‐dependent enzymes classified into the auxiliary activity (AA) families of the ...
Huang Z, Wei Q, Nan J, Nørholm MHH, Liu Z, Hernández-Rollán C, Johansen KS, Lo Leggio L.   +7 more
europepmc   +2 more sources

Dose-dependent structural and electron-density features in the lytic polysaccharide monooxygenase NcAA9D. [PDF]

Acta Crystallogr D Struct Biol
A series of 36 structures of the Neurospora crassa LPMO NcAA9D were refined against X‐ray crystal datasets collected with increasing dose from a single crystal to provide insights into dose‐dependent structural changes at the active site of the enzyme.
Miller SA, O'Dell WB, Meilleur F.
europepmc   +2 more sources

Redox robustness drives LPMO evolution. [PDF]

Proc Natl Acad Sci U S A
Enzymes known as lytic polysaccharide monooxygenases (LPMOs) are exceptionally powerful small redox enzymes that master the controlled generation and productive use of potentially damaging hydroxyl radicals in what is essentially a H 2 O 2 -driven ...
Ayuso-Fernández I, Emrich-Mills TZ, Golten O, Forsberg Z, Hall KR, Nagy LG, Sørlie M, Kjendseth Røhr Å, Eijsink VGH.   +8 more
europepmc   +5 more sources

Investigation Into the Role of Reductants and Cosubstrates in Lytic Polysaccharide Monooxygenase Thermothielavioides terrestris AA9E Binding to Cellulose by Single-Molecule Imaging. [PDF]

Biotechnol Bioeng
In glucose oxidase + catalase (GODCAT) and protocatechuic acid/protocatechuate 3,4‐dioxygenase (PCA/PCD) oxygen‐scavenging buffers, some Cy5‐TtAA9E bound to crystalline cellulose fibrils. Adding ascorbic acid (AscA) vastly increased enzyme binding to cellulose in the GODCAT buffer but diminished enzyme binding to cellulose in the PCA/PCD buffer ...
Blossom BM, Goodwin PM, Angeltveit CF, Horn SJ, Hitomi AO, Jeoh T.   +5 more
europepmc   +2 more sources

Lytic polysaccharide monooxygenase synergized with lignin-degrading enzymes for efficient lignin degradation

iScience, 2023
Summary: Even though the discovery of lytic polysaccharide monooxygenases (LPMOs) has fundamentally shifted our understanding of biomass degradation, most of the current studies focused on their roles in carbohydrate oxidation.
Su Sun, Fei Li, Muzi Li, Wenqian Zhang, Zhenxiong Jiang, Honglu Zhao, Yunqiao Pu, Arthur J. Ragauskas, Susie Y. Dai, Xiaoyu Zhang, Hongbo Yu, Joshua S. Yuan, Shangxian Xie   +12 more
doaj   +1 more source