Results 131 to 140 of about 341,486 (310)
miRNA-mRNA interaction conformed by luciferase assay.
, 2015 (A) The second structure of Y-47(custom-designed miRNA) and its potential target FSHB (for seed sequence only). (B) The second structure of ssc-miR-133a-3p and its potential target GNAI3 (for seed sequence only).
Ting Chen (15205) +12 more
core +1 more source
Advanced Science, EarlyView.Aberrant GALNT7‐mediated O‐GalNAcylation stabilizes TAZ to drive gallbladder cancer progression through a feed‐forward transcriptional loop. Structure‐based screening identifies Olaparib as a potent GALNT7 antagonist that disrupts this oncogenic axis, providing an immediate therapeutic strategy for this aggressive malignancy.
Peng Qiu +11 more
wiley +1 more source
Advanced Science, EarlyView.In the pathological context of osteoarthritis (OA), the phosphorylation of AKT1 at Ser473 enhances its binding to Lys140 of Insig1, which facilitates the formation of AKT1–Insig1 complex. Subsequently, the activation of AKT1 promotes the phosphorylation of Insig1 at Ser189, potentially enhancing the dissociation of Insig1 from sterol regulatory element‑
Xiaoqi Zhang +19 more
wiley +1 more source
Firefly luciferase titration data.
Raw data is provided for the firefly luciferase titrations in Fig 2A. Separate tabs denote data from the Bright-Glo assay condition and the Luciferase Assay System (LAS). (XLSX)
Axel Jacobsen (18004487) +12 more
core +1 more source
Dual-luciferase reporter assay.
, 2016 ATF4-WT indicates ATF4 wild-type luciferase.
Jing Yuan (217967) +5 more
core +1 more source
Advanced Science, EarlyView.This study unravels that bone fracture accelerates diabetic wound healing by releasing exosomal microRNA (miR‐130b‐3p). To mimic this bone‐skin crosstalk, we engineered a self‐assembling agomir‐130b‐3p nanocomplex. Delivered via a photocrosslinkable hydrogel, this bio‐inspired therapy provides sustained localized agomir release.
Tao Shen +14 more
wiley +1 more source
Coelenterazine Analogs for Bioassays and Molecular Imaging
SensorsCoelenterazine (CTZ) is a common substrate of marine luciferases upon emission of bioluminescence (BL) in living organisms. Because CTZ works as a “luminophore” in the process of BL emission, the chemical modification has been centered for improving the ...
Sung-Bae Kim +3 more
doaj +1 more source
Nicastrin knockdown NFKB-responsive luciferase assay
, 2019 These data are the luciferase assays of nicastrin knockdown cells with and without stimulation with TNF. The firefly luciferase was NFKB-responsive, and renilla luciferase was co-transfected as a transfection efficiency control.
Elisha Roberson (98532) +2 more
core +1 more source
Advanced Science, EarlyView.In TP53mut GBM cells, reduced P53 function is associated with increased TET1 expression. Genetic or pharmacological inhibition of TET1 correlates with genome fragility, including DNA damage, cellular senescence, telomere shortening, and reactive oxygen species accumulation, which may contribute to increased efficacy of antitumor therapy.
Zhuonan Pu +12 more
wiley +1 more source
Advanced Science, EarlyView.Engineering of the 148‐germline (148‐GL) antibody reveals a roadmap for overcoming SARS‐CoV‐2 viral escape. By introducing four specific somatic hypermutations, the resulting 148‐M4 variant restores potent neutralization against Omicron BA.1 and BA.4/5.
Huibin Lv +14 more
wiley +1 more source