Results 121 to 130 of about 341,486 (310)

Selenium Nanoparticles Selectively Target KRAS G13D to Inhibit Colorectal Cancer

Advanced Science, EarlyView.
The mechanisms of SeNPs therapy in cancer treatment, encompass three parallel actions: (1) seleno‐amino acids, key metabolites, upregulate GPX2 expression, thereby inhibiting tumor metastasis via the GPX2‐HIF1α‐VEGF signaling pathway; (2) selenite (SeO32−), an inorganic metabolite, forms hydrogen bonds with amino acid residues 13–17 of the KRAS G13D ...
Xiaoting Liu, Chong Wu, Shiyao Song, Li Ma, Shaoqing Huang, Yuting He, Qinghai Li, Yanzhou Chang, Dan Jiang, Yong Mei, Junchao Cai, Zhenshuang Du, Tianfeng Chen, Weiling He   +13 more
wiley   +1 more source

Decrease of luciferase activity.

, 2013
The expression level of luciferase was postoperatively observed using a noninvasive living image acquisition IVIS system. Accumulation of MSCs in the remnant liver. A. Most of the MSCs became trapped in the remnant liver.
Kouji Negishi (352292), Naoya Kasahara (328788), Shinji Uemoto (95909), Yasuhiro Fujimoto (352290), Takumi Teratani (52125), Tatsuaki Tsuruyama (289138), Eiji Kobayashi (107710), Junji Iwasaki (352291), Hiroyuki Kanazawa (352289)   +8 more
core   +1 more source

Mitochondrial SLC25A46 Rewires Fatty Acid Oxidation to Promote Cell Proliferation and Ferroptosis Evasion in Ovarian Cancer by Stabilizing CACT

Advanced Science, EarlyView.
Mitochondrial SLC25A46 is upregulated in ovarian cancer (OC) and correlates with poor prognosis. SLC25A46 stabilizes CACT to activate fatty acid oxidation, increasing ATP and NADPH production to drive cell proliferation and ferroptosis evasion. SLC25A46 silencing sensitizes OC cells to ferroptosis and carboplatin, offering a promising therapeutic ...
Yunge Gao, Jiatao Hao, Xiaohong Zhang, Meiting Long, Yujing Feng, Xiaoyue Liu, Xuejian Li, Shujuan Liu, Xin Guo, Xiaohui Lv   +9 more
wiley   +1 more source

Schematic of luciferase-based microRNA screen.

, 2012
293T cells were co-transfected with: 1) a vector containing a luciferase gene under control of regulatory elements recognized by AP-1, NF-κB, or p53 (in c-Myc screen, the E2F2-luc construct was used), 2) a member of our microRNA library, and 3) a Renilla
Wei Xiong (58411), Zhongxin Lu (121142), Weiqun Chen (121146), Maiying Kong (121148), Yong Li (15029), Lindsey E. Becker (121139)   +5 more
core   +1 more source

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source

Luciferase reporter assay.

, 2012
Transient transfection analysis for luciferase expression in A549 cells co-transfected with pre-miRNA precursors (pre-miRNAs) miR-27a, miR-144, miR-424*, or pre-miRNA precursor-Negative Control (pre-control), and reporter constructs containing the 3′UTR ...
Gabriele Sales (184799), Cristina Girardi (184779), Cristiano De Pittà (89055), Silvia Casara (184789), Lucia Celotti (184804), Maddalena Mognato (184807), Gerolamo Lanfranchi (10218)   +6 more
core   +1 more source

Gm26550 Modulates Learning and Memory by Increasing IGF1 mRNA Expression and Stability in Nrf2−/− Mice

Advanced Science, EarlyView.
The present study shows that Nrf2 directly binds to the Gm26550 promoter, thereby activating Gm26550 transcription and increasing its expression. Mechanistically, Gm26550 promotes IGF1 expression by functionally antagonizing miR‐26a‐5p‐mediated repression and sequestering the RBP KHSRP, thereby enhancing hippocampal neuronal synaptic plasticity and ...
Hongfang Wang, Ziyao Wang, Dongyue Zuo, Zhaowen Su, Bowen Song, Jiamin Gao, Yizhou Zhang, Ruiting Zhao, Linjie Sun, Mengdi Li, Yirui Fan, Dandan Geng, Lei Wang   +12 more
wiley   +1 more source

G-quadruplex regulation of Luciferase gene expression.

, 2015
(A) mRNA levels as assessed by qPCR and represented ΔΔCT values represents the normalization of the ΔCT of firefly luciferase to the ΔCT renilla luciferase then normalized to the wild type.
Brian P. Leung (831585), Neeraj P. Sebastian (831589), Michael R. Akins (831591), Aleister J. Saunders (218722), Chun Kit Kwok (831586), Reinhard Schweitzer-Stenner (831590), Ezekiel Crenshaw (831584), Michal Sharoni (831587), Kalee Olson (831588), Sara Ansaloni (218715), Philip C. Bevilacqua (384871)   +10 more
core   +1 more source

In Situ Genetic Engineering Facilitates MMP‐9‐Responsive TIMP1 Release Through Proteolytic Cleavage to Boost Diabetic Wound Healing

Advanced Science, EarlyView.
This study develops an MMP‐9‐responsive protein release system that transforms resident cells into drug depots through in situ genetic engineering. Anchored to the membrane, the therapeutic protein TIMP1 is selectively released upon MMP‐9 cleavage.
Yuan Zhang, Jiani Deng, Man Yee Cheung, Tingting Fu, Xufeng Qi, Dongqing Cai, Jianmin Sun, Gang Lu, Peng Shi, Wai‐Yee Chan, Xu Li, Hui Zhao   +11 more
wiley   +1 more source

Engineering a 660 nm-Responsive Optogenetic Inducer of Pyroptosis for Precision Cancer Therapy. [PDF]

Adv Sci (Weinh)
This study reports a bioorthogonal pyroptosis inducer, PyroRACS. By using a red‐light‐responsive Cre‐On genetic switch to control the expression of highly cytotoxic GSDMDNT, PyroRACS enables the artificial induction of pyroptosis with precise spatiotemporal control.
Zhang M, Li Z, Ma Y, Sun Y, Chen W, Huang W.   +5 more
europepmc   +2 more sources