Results 121 to 130 of about 325,683 (268)

Decrease of luciferase activity.

, 2013
The expression level of luciferase was postoperatively observed using a noninvasive living image acquisition IVIS system. Accumulation of MSCs in the remnant liver. A. Most of the MSCs became trapped in the remnant liver.
Kouji Negishi (352292), Naoya Kasahara (328788), Shinji Uemoto (95909), Yasuhiro Fujimoto (352290), Takumi Teratani (52125), Tatsuaki Tsuruyama (289138), Eiji Kobayashi (107710), Junji Iwasaki (352291), Hiroyuki Kanazawa (352289)   +8 more
core   +1 more source

Androgen Receptor‐Induced Lactoferrin Accelerates Prostate Tumorigenesis Through Modulating Ferroptosis

Advanced Science, EarlyView.
This study demonstrates that transcription factor androgen receptor (AR) directly binds the LF promoter, driving lactoferrin overexpression to promote ferritin (FTH1/FTL) upregulation and inhibit p53‐ALOX12‐mediated ferroptosis in prostate cancer. Lactoferrin could be a new potential therapeutic target in prostate cancer.
Can Liu, Qiu Peng, Xiaoyue Zhang, Zhengshuo Li, Yangge Wu, Yuqing Wen, Run Zheng, Shuanglong Wu, Chenfei Zhou, Zhengyu Hu, Lanjun Shu, Xinyi Ma, Qiuyang Li, Ya Ma, Chunlin Ou, Ming Zhao, Songqing Fan, Lingyu Wei, Jian Ma   +18 more
wiley   +1 more source

miRNA-mRNA interaction conformed by luciferase assay.

, 2015
(A) The second structure of Y-47(custom-designed miRNA) and its potential target FSHB (for seed sequence only). (B) The second structure of ssc-miR-133a-3p and its potential target GNAI3 (for seed sequence only).
Ting Chen (15205), Qing-Yan Jiang (290246), Qian-Yun Xi (290241), Xiao Cheng (203504), Chao-Yun Li (764427), Xiao-Tong Zhu (290239), Li-Na Wang (290233), Song-Bo Wang (290236), Rui-Song Ye (381554), Meng Li (79487), Qi-En Qi (764426), Gang Shu (172025), Yong-Liang Zhang (290243)   +12 more
core   +1 more source

Covalent Inhibition of SHMT2 by Gambogic Acid Induces Ferroptosis Through Mitochondrial Collapse in Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
In this study, chemoproteomics combined with genetic and functional analyses was integrated to identify SHMT2 as a covalent and functional target of gambogic acid (GA) in triple‐negative breast cancer (TNBC). Further validation demonstrated that GA selectively modifies the Cys241 site of SHMT2, triggering mitochondrial dysfunction, activating the Nrf2 ...
Tong Yang, Chong Qiu, Yulei Li, Ying Zhang, Chen Wang, Jie Zhou, Zheng Chu, Ang Ma, Ling Huang, Yin Kwan Wong, Junzhe Zhang, Peng Gao, Cui Liu, Junhua Zhang, Huan Tang, Jigang Wang   +15 more
wiley   +1 more source

G-quadruplex regulation of Luciferase gene expression.

, 2015
(A) mRNA levels as assessed by qPCR and represented ΔΔCT values represents the normalization of the ΔCT of firefly luciferase to the ΔCT renilla luciferase then normalized to the wild type.
Brian P. Leung (831585), Neeraj P. Sebastian (831589), Michael R. Akins (831591), Aleister J. Saunders (218722), Chun Kit Kwok (831586), Reinhard Schweitzer-Stenner (831590), Ezekiel Crenshaw (831584), Michal Sharoni (831587), Kalee Olson (831588), Sara Ansaloni (218715), Philip C. Bevilacqua (384871)   +10 more
core   +1 more source

m6A‐Mediated Glycolysis by IL‐37 Drives T Cell Metabolic Reprogramming to Regulate Colitis

Advanced Science, EarlyView.
This study identifies an IL‐37/SIGIRR‐METTL14 regulatory axis that suppresses global m6A modification in CD4+ T cells. IL‐37 signaling, mediated through SIGIRR, inhibits IRAK4 and JNK phosphorylation, leading to downregulation of the methyltransferase METTL14.
Xiaoyan Wang, Jiadong Yu, Guolin Li, Ya Li, Fanlian Zeng, Jing Hu, Yifan Zhou, Chengcheng Yue, Qixiang Zhao, Chen Zhang, Hong Zhou, Fulei Zhao, Yawen Hu, Pei Zhou, Linna Gu, Yuting Feng, Mingxiang He, Shishi Huang, Xiaochi Sun, Xinmeng Wang, Jie Tang, Xinyu Zeng, Wenling Wu, Nongyu Huang, Xinai Cui, Kaijun Cui, Jiong Li   +26 more
wiley   +1 more source

Redox‐Dependent Chaperoning of GBF1 Condensates Regulates Seed Germination in Arabidopsis

Advanced Science, EarlyView.
In dormant seeds (low ROS), GBF1 forms liquid condensates to repress the germination gene CathB3, and the chaperone GIP1 maintains condensate liquidity and repressive activity. Upon imbibition (high ROS), ROS oxidize GIP1 during germination, impairing its chaperone function.
Yunying Wang, Xiaofeng Fang
wiley   +1 more source

Dual-luciferase reporter assay.

, 2016
ATF4-WT indicates ATF4 wild-type luciferase.
Jing Yuan (217967), Meixia Fang (747684), Qingyun Xu (2574106), Ruixue Chen (3045180), Liguo Chen (3045183), Ling He (282204)   +5 more
core   +1 more source

Genome‐Wide In Vivo RNAi Screening Identifies HOXD4 as a Tumor Metastasis Suppressor in Colorectal Cancer

Advanced Science, EarlyView.
Metastasis remains a major challenge in colorectal cancer. Using an in vivo shRNA screening system, this study identifies Homeobox D4 as a key metastasis suppressor. Reduced Homeobox D4 expression is associated with aggressive tumor features. Functional and mechanistic analyses show that it inhibits epithelial‐mesenchymal transition by repressing ...
Zhi‐hua Ye, Wen‐jing Luo, Lu Li, Wen‐di Shuai, Ling Zhou, You‐fa Duan, Xue Chen, Jun‐kai Zhang, Wenlin Huang, Ran‐yi Liu   +9 more
wiley   +1 more source

Firefly luciferase titration data.

Raw data is provided for the firefly luciferase titrations in Fig 2A. Separate tabs denote data from the Bright-Glo assay condition and the Luciferase Assay System (LAS). (XLSX)
Axel Jacobsen (18004487), Prasenjit Mondal (1415635), Rafael Gómez-Sjöberg (2467432), Paul Lebel (11250722), Ilakkiyan Jeyakumar (18004481), Senjuti Saha (174204), Chris Charlton (7167701), Diane M. Wiener (1956583), Emily Huynh (16926354), Cristina M. Tato (12074204), Susanna Elledge (18004478), Maíra Phelps (18004484), Robert Puccinelli (6167315)   +12 more
core   +1 more source