Results 101 to 110 of about 417,647 (313)

rWTC‐MBTA Vaccine, Alone and Enhanced with Anti‐PD1, Elicits Immune Responses against CNS and Peripheral B‐Cell Lymphoma

Advanced Science, EarlyView.
An autologous whole‐tumor‐cell vaccine (rWTC‐MBTA) is evaluated in murine CNS lymphoma. Subcutaneous vaccination activates dendritic cells, broadens T‐cell priming, and drives lymphocyte trafficking to brain tumors, producing durable tumor control. Longitudinal bioluminescence and adoptive‐transfer assays verify CNS engagement. Combination with anti‐PD‐
Yaping Zhang, Juan Ye, Mitchell Sun, Samik Chakraborty, Alex Valenzuela, Shuran Chen, Qingfeng Xue, Wenyu Shi, Karel Pacak, Herui Wang, Zhengping Zhuang   +10 more
wiley   +1 more source

Critical parameters for robust Agrobacterium‐mediated transient transformation and quantitative promoter assays in Catharanthus roseus seedlings

Plant Direct
Agrobacterium‐mediated transient expression methods are widely used to study gene function in both model and non‐model plants. Using a dual‐luciferase assay, we quantified the effect of Agrobacterium‐infiltration parameters on the transient ...
Lauren F. Cole‐Osborn, Emma Meehan, Carolyn W. T. Lee‐Parsons   +2 more
doaj   +1 more source

Modification of H1N1 Influenza Luciferase Reporter Viruses Using StopGo Translation and/or Mouse-Adapted Mutations

Viruses
Reporter viruses are valuable tools for studying infections at the cellular level and in living animals. They also enable rapid, high-throughput antiviral drug screening and serological studies.
Po-Ling Chen, Guohua Yang, Chet Ojha, Balaji Banoth, Charles J. Russell   +4 more
doaj   +1 more source

Quantification of Lyssavirus-Neutralizing Antibodies Using Vesicular Stomatitis Virus Pseudotype Particles

Viruses, 2016
Rabies is a highly fatal zoonotic disease which is primarily caused by rabies virus (RABV) although other members of the genus Lyssavirus can cause rabies as well. As yet, 14 serologically and genetically diverse lyssaviruses have been identified, mostly
Sarah Moeschler, Samira Locher, Karl-Klaus Conzelmann, Beate Krämer, Gert Zimmer   +4 more
doaj   +1 more source

Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions [PDF]

, 2010
Gene therapy is expected to open a new strategy for the treatment of refractory vascular diseases, so the development of appropriate gene vectors for vascular lesions is needed. To realize this requirement with a non-viral approach, cyclo(RGDfK) peptide (
Hideo Kagaya, Hiroyuki Koyama, Kazunori Kataoka, Makoto Oba, Takehiko Ishii, Tetsuro Miyata, Tsuyoshi Takato, Yutaka Miura   +7 more
core   +1 more source

Substrate Cooperativity in Marine Luciferases

PLoS ONE, 2012
Marine luciferases are increasingly used as reporters to study gene regulation. These luciferases have utility in bioluminescent assay development, although little has been reported on their catalytic properties in response to substrate concentration.
George Tzertzinis, Ezra Schildkraut, Ira Schildkraut   +2 more
openaire   +4 more sources

GATA4‐Driven Transcription of HtrA1 Promotes Cellular Senescence in Ménière's Disease and Age‐Related Audio‐Vestibular Dysfunction

Advanced Science, EarlyView.
This study identifies the HDAC6/GATA4/HtrA1 axis as a critical driver of cellular senescence in the inner ear. GATA4 nuclear translocation, facilitated by HDAC6 downregulation, transcriptionally activates HtrA1, promoting hair cell senescence, SASP, and audio‐vestibular dysfunction in models of Ménière's disease and age‐related audio‐vestibular ...
Na Zhang, Na Li, Yan Wang, Jing Zhang, Jiahui Liu, Lei Chen, Yongdong Song, Yurong Mu, Yuechen Han, Yafeng Lyu, Xiaofei Li, Hanyue Wang, Jing Wang, Yao Lu, Zhaomin Fan, Daogong Zhang, Haibo Wang   +16 more
wiley   +1 more source

A cost-effective dual reporter system in Nicotiana benthamiana

Frontiers in Plant Science
Dual reporter assay has been widely used for quantification of gene expression in various model systems. However, the assay is dependent on commercial kits, which may be inhibitory for many low-budget academic laboratories or high throughput screening ...
Zeru Zhang, Shuangle Yan, Fuqi Li, Zhijuan Rao, Fahong Fan, Haibin Ren, Yanlin Qu, Ke Yang, Chuanyu Ma, Chen Zhuang, Chentao Lin, Gaoping Qu   +11 more
doaj   +1 more source

Dynein Regulators Are Important for Ecotropic Murine Leukemia Virus Infection [PDF]

, 2016
Indexación: Web of Science.During the early steps of infection, retroviruses must direct the movement of the viral genome into the nucleus to complete their replication cycle.
Arriagada, Gloria, Cancino, Jorge, Goff, Stephen P., Opazo, Tatiana, Valle-Tenney, Roger   +4 more
core   +1 more source

Natural Products Inspired Scaffold Diversification Leads to Unnatural Molecular Warhead and Covalent Strategy to Modulating Protein Function through Electrophilic Bromine Transfer

Advanced Science, EarlyView.
We report a new thiolate‐reactive α,α‐gem‐dibromo lactam warhead that activates transcription factor Nrf2 and demonstrates anti‐inflammatory activities, which have implications in cancer, neurodegeneration, and cardiovascular diseases. RNA‐seq illuminated detailed transcriptional profiles, and chemical reactions with cysteine‐containing compounds ...
Beau R. Brummel, Sofia Kokkaliari, Qiwen Gao, Jinjing Chen, Larissa Costa de Almeida, Srinivasarao Tenneti, Derek A. Leas, Eli Levit, Lewis S. Alexander, Khalil A. Abboud, Pingrong Wei, Michael D. Cameron, James R. Rocca, Ranjala Ratnayake, Donna D. Zhang, Hendrik Luesch, Robert W. Huigens III   +16 more
wiley   +1 more source