Results 101 to 110 of about 341,486 (310)

Development of a high-throughput screening platform for C. difficile toxin synthesis inhibitors unveils meclizine as an antivirulence agent

Antimicrobial Agents and Chemotherapy
Clostridioides difficile, a leading cause of hospital-acquired diarrhea, exerts its virulence through two co-regulated toxins, TcdA and TcdB. Despite their pivotal roles, the discovery of inhibitors targeting their biosynthesis is underexplored. Here, we
Ravi K. R. Marreddy, Nghi Nguyen, Chetna Dureja, Ann Marie McKelvey, Reid Powell, Abiola O. Olaitan, Clifford Stephan, Julian G. Hurdle   +7 more
doaj   +1 more source

Luciferase activity assay.

, 2013
Luciferase reporter constructs were generated through placing fragments of 185 bp (Construct I, sequence from nt31–216 of the VEGF 3′-UTR) and 241 bp (Construct II, sequence from nt703–944 of the VEGF 3′-UTR) at the 3′ end of luciferase gene in pRL-TK ...
Zhong Hua (63061), Yaou Zhang (63065), Wenbin Ye (63056), Dayong Gu (274142), Burton B. Yang (63064), Qing Lv (63057), Guoping Cai (63062), Chung-Kwun Amy Wong (63058), Yanhong Ji (274143), Jifeng Wang (274144), Chen Zhao (225070)   +10 more
core   +1 more source

Chemical Screening Identifies Danofloxacin as a Self‐Renewal Agonist of Embryonic Stem Cells through Alleviation of HDAC1‐Mediated Deacetylation of Tert and Prdm10

Advanced Science, EarlyView.
This study identified Danofloxacin as a novel supporter of the maintenance of mouse embryonic stem cell (ESC) pluripotency. It functions partially by inhibiting HDAC1, which increases specific histone acetylation marks and activates key genes like Tert and Prdm10.
Yan Zhang, Junxiang Ji, Baoxing Dong, Zhenhua Zhu, Xucheng Lv, Peng Chen, Wenqiang Liu, Min Zhou, Shou‐Dong Ye   +8 more
wiley   +1 more source

Coixenolide Enhances Antitumor Immunity of Cytotoxic CD8+ T Cells by Inhibiting S100A8/A9‐CD36 Axis in Obesity‐Associated Pancreatic Adenocarcinoma

Advanced Science, EarlyView.
Pancreatic adenocarcinoma (PAAD) remains highly lethal with limited treatment options. This study demonstrates that coixenolide, a bioactive compound from Coix lacryma‐jobi L. and a key component of the clinically approved Kanglaite injection, exhibits enhanced antitumor efficacy in high‐fat diet (HFD)‐induced obese mice bearing PAAD tumors compared to
Kaidi Chen, Rong Chen, Wei Li, Bin Song, Ruiying Zhang, Lewei Dai, Yuwei Qi, Ruqian Chen, Yifan Xu, Hangbin Li, Jingyi Huang, Fangping Wu, Jianan Peng, Xinyu Wang, Yang Xiong, Chaofeng Mu, Guilin Cheng, Zhaoshen Li, Shanming Ruan, Lei Huang, Mancang Gu   +20 more
wiley   +1 more source

Regulation of WDFY1 Expression by miRNAs, Transcription Factors, and IL-6 in Murine Mesangial Cells

Cells
WD40 repeat and FYVE containing protein 1 (WDFY1) functions in membrane trafficking and protein complex scaffolding. WDFY1 has been studied in the immune system and in different oncogenic conditions.
David E. Adams, Siru Li, Yuxuan Zhen, Ahmet Kaynak, Xiaoyang Qi, Jane J. Yu, Wen-Hai Shao   +6 more
doaj   +1 more source

Compensatory Mitophagy via NEDD4/HIF‐1α/BNIP3 Pathway Restrains EndMT and Renal Allograft Interstitial Fibrosis Induced by TNFα

Advanced Science, EarlyView.
The role of TNFα in the process of renal allograft interstitial fibrosis is complex and multifaceted. As it can promote renal allograft interstitial fibrosis by inducing mitochondrial dysfunction and EndMT, and also mediating compensatory mitophagy through the NEDD4–HIF‐1α–BNIP3 pathway, which clears damaged mitochondria and inhibits EndMT, thereby ...
Dengyuan Feng, Qinghuan Shen, Junqi Zhang, Jiawen Liu, Zeping Gui, Zijie Wang, Qianguang Han, Shuang Fei, Hao Chen, Li Sun, Jun Tao, Zhijian Han, Xiaobing Ju, Min Gu, Jianjian Zhang, Ruoyun Tan   +15 more
wiley   +1 more source

Visualization of Leishmania tropica Infection in BALB/c Mice by Bioluminescence Imaging

Iranian Biomedical Journal, 2020
Background: Leishmania tropica is the cause of more than one form of leishmaniasis and lacks a known reservoir animal. This study compares the potential infectivity of recombinant and wild-type L. tropica in BALB/c mice.
Mahdieh Eskandar, Elham Gholami, Negar Seyed, Yasaman Taslimi, Sima Habibzadeh, Sima Rafati, Tahereh Taheri   +6 more
doaj  

Oxygen-sensitive luciferase reporter system.

, 2012
(A) Scheme of luciferase constructs for constitutive (Luc) and oxygen-sensitive luciferase expression (ODD-Luc), containing a part of the oxygen-dependent degradation (ODD) domain of HIF-1α.
Martina Zimmermann (112724), Van Vuong (112729), Carla Rohrer Bley (112718), Katrin Orlowski (112713), Angela Broggini-Tenzer (112742), Daniel Hug (112733), Alex Soltermann (112737), Martin Pruschy (112745)   +7 more
core   +1 more source

Modulation of miR‐23b Wnt/β‐catenin Axis Strengthens Endothelial Barrier Properties

Advanced Science, EarlyView.
Early blood‐brain barrier (BBB) disruption contributes to stroke and CNS disease pathology. miR‐23b was identified as a regulator of BBB integrity in brain endothelial cells. Inhibition of miR‐23b enhanced barrier‐associated properties, promoted repair‐related signaling, and reduced BBB leakage in experimental stroke models, supporting further ...
Victor Anthony Martinez, Rose E. Presby, Sara Nakanishi, Pallavi Gaur, Ugur Akcan, Johannes Daugaard Krog, Iben Daugaard Krog, Lipi Das, David S. Johnson, Danny Jamoul, Douglas Jury, Brian Lawson, Jacob Körbelin, Lewis M. Brown, Vilas Menon, Dritan Agalliu, Irene Munk Pedersen   +16 more
wiley   +1 more source

Dual Luciferase Reporter Gene

, 2011
Luciferase is an enzyme that is used in bioluminescence. Luciferase most famous form is the one found in fireflies. In this form Luciferase works in a very unique way.
Bolanos, Edwin, Bach, Christian
core   +1 more source