Results 91 to 100 of about 341,486 (310)

Functional Characterisation and Analysis of the Soluble NKG2D Ligand Repertoire Detected in Umbilical Cord Blood Plasma

open access: yesFrontiers in Immunology, 2018
We previously reported that cord blood plasma (CBP) contains significantly more soluble NKG2D ligands (sNKG2DLs), such as sMICB and sULBP1, than healthy adult plasma.
Steven T. Cox   +9 more
doaj   +1 more source

Relative luciferase activity.

open access: yes, 2014
The light emission of luciferase was measured after addition of Luciferase Assay Reagent II, normalized against the protein content in samples transfected by pGL4-Irx3 (Irx), promoterless pGL4.12 (pB), pGL4-Irx3 plus increasing molar ratio of Nkx2.2 (N1,
András Dinnyés (4635640)   +6 more
core   +1 more source

Membrane‐Bound Multimodal Plasmonic Transducers for Noninvasive, In Situ Monitoring and Control of CAR T Cells Against Heterogeneous Solid Tumors

open access: yesAdvanced Materials, EarlyView.
Membrane‐bound multimodal transducers enable longitudinal ultrasound‐guided photoacoustic monitoring and in situ thermal control of CAR T cells in heterogeneous solid tumors. Imaging correlates T cell trafficking with tumor response, distinguishing responders from nonresponders.
Myeongsoo Kim   +14 more
wiley   +1 more source

A cost-effective dual reporter system in Nicotiana benthamiana

open access: yesFrontiers in Plant Science
Dual reporter assay has been widely used for quantification of gene expression in various model systems. However, the assay is dependent on commercial kits, which may be inhibitory for many low-budget academic laboratories or high throughput screening ...
Zeru Zhang   +11 more
doaj   +1 more source

Chemical and biological investigation of the Agaricus silvaticus Schaeff ex. Secr

open access: yesMongolian Journal of Chemistry, 2014
Extract of Agaricus silvaticus Schaeff ex. Secr fungus have been known to have biological activities, including antibacterial, antiviral, anticancer, antioxidant, anticomplementary and immunostimulating effects.
L Munkhgerel   +5 more
doaj   +1 more source

Esterified Cholesterol Conjugates Enable LNP‐Mediated mRNA Delivery to the Blood–Brain Barrier Following Intravenous Administration

open access: yesAdvanced Materials, EarlyView.
A cholesterol‐conjugated lipid library enabled the identification of a ligand‐free LNP platform for efficient mRNA delivery to brain endothelial cells via systemic administration. This platform achieves selective BBB targeting without disrupting barrier integrity, and enables modulation of neuroinflammation and vascular function without requiring trans‐
Zeru Tian   +7 more
wiley   +1 more source

Analysis of in vivo infection dynamics using bioluminescent Giardia duodenalis

open access: yesInfection and Immunity
Giardia duodenalis is an intestinal protozoan transmitted through contaminated food and water and is a major cause of diarrheal disease worldwide and has been linked to post-infectious sequelae and growth impairment in children.
Rita Kosile   +6 more
doaj   +1 more source

Quantification of Lyssavirus-Neutralizing Antibodies Using Vesicular Stomatitis Virus Pseudotype Particles

open access: yesViruses, 2016
Rabies is a highly fatal zoonotic disease which is primarily caused by rabies virus (RABV) although other members of the genus Lyssavirus can cause rabies as well. As yet, 14 serologically and genetically diverse lyssaviruses have been identified, mostly
Sarah Moeschler   +4 more
doaj   +1 more source

LDLR luciferase constructs and SNP luciferase activity in Huh7 cell line.

open access: yes, 2016
A) Schematic presentation of LDLR-luciferase-construct (promoter only) and LDLR-luciferase-enhancer-constructs. The constructs were transfected into Huh7 cells.
Jon White (44386)   +4 more
core   +1 more source

Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency

open access: yesAdvanced Materials, EarlyView.
As lipid nanoparticle (LNP) systems are the leading platform for in vivo nucleic acid delivery, improving transfection potency of these systems is of considerable interest. In this work, we explored a post‐mixing formulation method involving a two‐step buffer exchange that increased the number of mRNA copies within each LNP while reducing the number of
Yao Zhang   +12 more
wiley   +1 more source

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