Results 91 to 100 of about 325,683 (268)

Quantification of Lyssavirus-Neutralizing Antibodies Using Vesicular Stomatitis Virus Pseudotype Particles

Viruses, 2016
Rabies is a highly fatal zoonotic disease which is primarily caused by rabies virus (RABV) although other members of the genus Lyssavirus can cause rabies as well. As yet, 14 serologically and genetically diverse lyssaviruses have been identified, mostly
Sarah Moeschler, Samira Locher, Karl-Klaus Conzelmann, Beate Krämer, Gert Zimmer   +4 more
doaj   +1 more source

Gut Mycobiota‐Associated Tryptophan Catabolites Protect Against Metabolic Dysfunction‐Associated Steatotic Liver Disease

Advanced Science, EarlyView.
ABSTRACT Accumulating evidence suggests that the intestinal microbiota participates in the progression of metabolic dysfunction‐associated steatotic liver disease (MASLD) through microbiota‐host interaction. However, the beneficial role of commensal mycobiota in MASLD progression remains poorly understood.
Shuping Qiao, Shuangya Fan, Juan Xu, Zhen Xu, Chen Peng, Junxing Qu, Ziqian Bing, Shizhen Zhou, Sunan Shen, Guifang Xu, Yue Zhao, Tingting Wang   +11 more
wiley   +1 more source

Luciferase activity assay.

, 2013
Luciferase reporter constructs were generated through placing fragments of 185 bp (Construct I, sequence from nt31–216 of the VEGF 3′-UTR) and 241 bp (Construct II, sequence from nt703–944 of the VEGF 3′-UTR) at the 3′ end of luciferase gene in pRL-TK ...
Zhong Hua (63061), Yaou Zhang (63065), Wenbin Ye (63056), Dayong Gu (274142), Burton B. Yang (63064), Qing Lv (63057), Guoping Cai (63062), Chung-Kwun Amy Wong (63058), Yanhong Ji (274143), Jifeng Wang (274144), Chen Zhao (225070)   +10 more
core   +1 more source

Assessment of the stability of TGFβ3 bioactivity for potential bioreactor applications [PDF]

, 2008
In order to develop suitable bioreactor systems and processes for automated and standardized cell cultures involving the use of bioactive factors, we determined the stability of transforming growth factor beta 3 (TGF beta 3) over storage time and under ...
Vonwil, D., Stroebel, S., Wallny, H. J., Martin, I., Gygax, D., Heberer, M., Wendt, D.   +6 more
core   +1 more source

A cost-effective dual reporter system in Nicotiana benthamiana

Frontiers in Plant Science
Dual reporter assay has been widely used for quantification of gene expression in various model systems. However, the assay is dependent on commercial kits, which may be inhibitory for many low-budget academic laboratories or high throughput screening ...
Zeru Zhang, Shuangle Yan, Fuqi Li, Zhijuan Rao, Fahong Fan, Haibin Ren, Yanlin Qu, Ke Yang, Chuanyu Ma, Chen Zhuang, Chentao Lin, Gaoping Qu   +11 more
doaj   +1 more source

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

Advanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, Xin Feng, Hongzhang Cui, Menghan Luo, Tielong Yang, Liuxing Wu, Ke Zhao, Weilong Yang, Mulin Jun Li, Dandan Huang, Jilong Yang   +19 more
wiley   +1 more source

LDLR luciferase constructs and SNP luciferase activity in Huh7 cell line.

, 2016
A) Schematic presentation of LDLR-luciferase-construct (promoter only) and LDLR-luciferase-enhancer-constructs. The constructs were transfected into Huh7 cells.
Jon White (44386), Roaa Hani Fairoozy (3545228), Jutta Palmen (142608), Anastasia Z. Kalea (315611), Steve E. Humphries (142616)   +4 more
core   +1 more source

A Rare Allele of ST5 From Wild Rice Enhances Salt Tolerance in Rice

Advanced Science, EarlyView.
A novel salt‐tolerance gene ST5W, characterized by a unique 36‐bp promoter insertion, is found exclusively in a small subset of Oryza rufipogon and is absent in cultivated rice. Field trials confirm ST5W significantly enhances rice yield across diverse genetic backgrounds under saline conditions.
Meng Xing, Jingfen Huang, Qiaoling Yuan, Ziyi Yang, Yanyan Wang, Mingchao Zhao, Yamin Nie, Rui Xu, Hongge Qian, Wenxi Chen, Qiaoling Zhang, Qi Du, Leiyue Geng, Yapeng Li, Ziyi Chen, Shizhuang Wang, Like Lou, Haiyuan Peng, Chongke Zheng, Xianzhi Xie, Xiaoming Zheng, Lifang Zhang, Lianguang Shang, Jiaqiang Sun, Qian Qian, Qingwen Yang, Weihua Qiao   +26 more
wiley   +1 more source

Regulation of WDFY1 Expression by miRNAs, Transcription Factors, and IL-6 in Murine Mesangial Cells

Cells
WD40 repeat and FYVE containing protein 1 (WDFY1) functions in membrane trafficking and protein complex scaffolding. WDFY1 has been studied in the immune system and in different oncogenic conditions.
David E. Adams, Siru Li, Yuxuan Zhen, Ahmet Kaynak, Xiaoyang Qi, Jane J. Yu, Wen-Hai Shao   +6 more
doaj   +1 more source

Targeting the PDK1/c‐Myc/SOX10 Signaling in Oligodendrocytes Alleviates Neuropathic Pain

Advanced Science, EarlyView.
This work reveals that oligodendrocyte homeostasis, mediated by PDK1, is a critical determinant of neuropathic pain (NPP) pathogenesis. Disruption of PDK1 in oligodendrocytes impairs SOX10‐dependent myelination programs through c‐Myc accumulation, leading to disrupted myelination and the pathophysiology of NPP.
Pingping Qiao, Lifang Guo, Guochao Yang, Chaoli Huang, He Wang, Jian‐Jun Yang, Guiquan Chen, Yimin Hu   +7 more
wiley   +1 more source