Results 111 to 120 of about 325,683 (268)

Modified luciferase

, 2007
The invention comprises modified luciferase proteins which are more resistant to inhibition by test chemicals than wild type luciferase. The modified luciferases also contain greater thermostability than wild type luciferase.https://digitalcommons.mtu ...
Shonnard, David R., Kim Choi, Eileen H., Rybina, Irina V.   +2 more
core  

Luciferase protein determination with western blotting analysis of luciferase library.

, 2013
(A) A typical western result of TCA precipitated yeast culture supernatants of luciferase library with variable P1’ site, all tested recombinant yeast strains were randomly picked with zeocin resistance ranging 500-1,000 µg/ml, 5 µg of supernatant ...
Xiaoyan Hui (422523), Pentao Liu (31036), Zhiwu Jiang (460654), Shiwu Li (460656), Ye Kuang (460652), Peng Li (69518), Hongbo Li (130443), Aimin Xu (120142), Yuehong Liu (460653), Donghai Wu (309245), Song Yang (162335), Yu Wang (12152), Yulai Zhou (460655)   +12 more
core   +1 more source

Hijacking the Host Clock: A Nematode Effector Antagonizes Soybean Circadian Defense and Translation Control

Advanced Science, EarlyView.
Soybean employs its circadian clock, governed by GmCCA1, to rhythmically defend against soybean cyst nematodes. The pathogen retaliates by secreting the effector Hg4E02, which hijacks the clock to suppress defense and co‐opt the host's translation machinery for nutrient acquisition.
Xingwei Wang, Yufeng Xu, Yanfei Hu, Lijun Cao, Ru Jiang, Changtian Chen, Rick Masonbrink, Thomas Maier, Yuchen Tu, Yabo Shi, Enhui Liu, Lingan Kong, Chan Guo, Wei Zhao, Peng Shi, Wenzhen Du, Andrew Severin, Thomas Baum, Deliang Peng, Huan Peng, Mian Zhou, Wei Wang   +21 more
wiley   +1 more source

Luciferase reporter assay.

, 2012
Transient transfection analysis for luciferase expression in A549 cells co-transfected with pre-miRNA precursors (pre-miRNAs) miR-27a, miR-144, miR-424*, or pre-miRNA precursor-Negative Control (pre-control), and reporter constructs containing the 3′UTR ...
Gabriele Sales (184799), Cristina Girardi (184779), Cristiano De Pittà (89055), Silvia Casara (184789), Lucia Celotti (184804), Maddalena Mognato (184807), Gerolamo Lanfranchi (10218)   +6 more
core   +1 more source

Circular RNA circNrip1 Interacts with SYNCRIP to Promote Neuropathic Pain by Stabilizing Tlr2 mRNA in Primary Sensory Neurons

Advanced Science, EarlyView.
Model of circNrip1 (cNrip1) upregulation driving neuropathic pain mechanisms. After peripheral nerve injury, increased FUS triggers the formation and upregulation of cNrip1 in injured DRG neurons. Upregulated cNrip1 recruits SYNCRIP to the 3′‐UTR of Tlr2 mRNA by binding to both, thereby promoting SYNCRIP‐triggered Tlr2 mRNA stability and increasing ...
Xiaozhou Feng, Jifang Xiao, Congcong Huang, Bao‐Chun Jiang, Bing Wang, Xu Li, Shibin Du, Tolga Berkman, Chun‐Hsien Wen, Weihua Cai, Dilip Sharma, Kun Wang, Longfei Ma, Huijuan Hu, Yuan‐Xiang Tao   +14 more
wiley   +1 more source

Corrigendum: Functional Characterisation and Analysis of the Soluble NKG2D Ligand Repertoire Detected in Umbilical Cord Blood Plasma

Frontiers in Immunology, 2020
Steven T. Cox, Steven T. Cox, Robert Danby, Robert Danby, Diana Hernandez, Diana Hernandez, Raquel Laza-Briviesca, Hayley Pearson, J. Alejandro Madrigal, J. Alejandro Madrigal, Aurore Saudemont, Aurore Saudemont   +11 more
doaj   +1 more source

Inhibition of SIRT7 Overcomes Radioresistance in Pancreatic Neuroendocrine Tumors by Reactivating MEN1 Expression

Advanced Science, EarlyView.
Pancreatic neuroendocrine tumors frequently silence MEN1 through epigenetic mechanisms. Here, SIRT7 recruits DNMT1 to the MEN1 promoter, drives hypermethylation, and enhances DNA repair. Inhibiting SIRT7 restores MEN1, reduces MRN complex abundance, impairs double‐strand break repair, and sensitizes PanNET models to radiation, supporting SIRT7 as a ...
Jianyun Jiang, Yan Wang, Yi Qin, Luohai Chen, Xiaowu Xu, Guixiong Fan, Desheng Jing, Miaoyan Wei, Xianjun Yu, Junfeng Xu, Shunrong Ji, Jie Chen   +11 more
wiley   +1 more source

Schematic of luciferase-based microRNA screen.

, 2012
293T cells were co-transfected with: 1) a vector containing a luciferase gene under control of regulatory elements recognized by AP-1, NF-κB, or p53 (in c-Myc screen, the E2F2-luc construct was used), 2) a member of our microRNA library, and 3) a Renilla
Wei Xiong (58411), Zhongxin Lu (121142), Weiqun Chen (121146), Maiying Kong (121148), Yong Li (15029), Lindsey E. Becker (121139)   +5 more
core   +1 more source

Tumor‐Derived LAMB3 Drives Immunosuppressive LRRC15+ Fibroblast Formation During Pancreatic Ductal Adenocarcinoma Development

Advanced Science, EarlyView.
A single‐cell atlas of pancreatic ductal adenocarcinoma development reveals progressive ductal‐fibroblast‐immune crosstalk. Tumor‐derived LAMB3 drives the formation of immunosuppressive LRRC15+ fibroblasts through the ITGB1/FAK/MAPK/FOSL2 signaling. Glycolytic reprogramming upregulates LAMB3 and correlates with LRRC15+ fibroblast enrichment.
Xuqing Shi, Hangqi Liu, Jianru Sun, Xiaoding Liu, Xinping Jv, Longyun Chen, Yuhan Zhang, Hui Zhang, Xudong Xing, Ruiyu Li, Xinyi Ke, Jun Wang, Xianglin Yin, Bohan Liu, Qixian Liu, Yuan Wang, Junliang Lu, Shiyi Liu, Junyi Pang, Yumeng Cai, Menghua Dai, Fan Bai, Huanwen Wu, Zhiyong Liang   +23 more
wiley   +1 more source

Dual Luciferase Reporter Gene

, 2011
Luciferase is an enzyme that is used in bioluminescence. Luciferase most famous form is the one found in fireflies. In this form Luciferase works in a very unique way.
Bolanos, Edwin, Bach, Christian
core