Results 81 to 90 of about 341,486 (310)

Modified luciferase [PDF]

, 2007
The invention comprises modified luciferase proteins which are more resistant to inhibition by test chemicals than wild type luciferase. The modified luciferases also contain greater thermostability than wild type luciferase.https://digitalcommons.mtu ...
Shonnard, David R., Kim Choi, Eileen H., Rybina, Irina V.   +2 more
core  

A Holistic Platform Approach for Developing Robust Pulmonary mRNA Delivery: Integrating Novel Phenolic Acid‐Derived Ionizable Lipids and Device Engineering

Advanced Functional Materials, EarlyView.
An integrated pulmonary mRNA delivery platform combining novel biodegradable syringic acid‐derived ionizable lipids, design‐of‐experiments formulation optimization, and vibrating‐mesh nebulizer engineering enabled stable aerosolization and efficient lung delivery.
Neha Kaushal, Younan Ma, Asad Khanmohammed, Dipen Parande, Hanming Zhang, Saket Dolare, Juliet Chen, Apiwat Wangweerawong, Saswata Karmakar, Hongfeng Deng, Frank Koppenhagen, Shraddha Sharma, Eric Reyes, Sherry He, Lianne Boeglin, Caroline Woo, Azadeh Paksa, Ayed Allawzi, Anusha Dias, Gregory Troiano, Shrirang Karve, Frank DeRosa   +21 more
wiley   +1 more source

Engineering recombinant replication-competent bluetongue viruses expressing reporter genes for in vitro and non-invasive in vivo studies

Microbiology Spectrum
Bluetongue virus (BTV) is the causative agent of the important livestock disease bluetongue (BT), which is transmitted via Culicoides bites. BT causes severe economic losses associated with its considerable impact on health and trade of animals.
Sergio Utrilla-Trigo, Luis Jiménez-Cabello, Alejandro Marín-López, Miguel Illescas-Amo, Germán Andrés, Eva Calvo-Pinilla, Gema Lorenzo, Piet A. van Rijn, Javier Ortego, Aitor Nogales   +9 more
doaj   +1 more source

Imaging Single Photons and Intrinsic Optical Signalsfor Studies of Vesicular and Non-Vesicular ATP Release from Axons

Frontiers in Neuroanatomy, 2011
The temporal and spatial dynamics of neurotransmitter release are fundamental to understanding activity-dependent signaling between axons and other cells, including neurons, glia, and vascular cells.
Douglas eFields
doaj   +1 more source

Luciferase activity assessed by the Dual Luciferase Reporter Assay System.

, 2015
a) Bambi confirmed as the direct target gene of miR-20b. 20b+Bambi: the luciferase activity of Bambi in the miR-20b overexpression group; NC+Bambi: the luciferase activity of Bambi in the control; 20b+mut: the luciferase activity of mutated Bambi in the ...
Shuping Han (112521), Yuzhu Peng (728099), Zhangbin Yu (112518), Shasha Zhu (471439), Xiaoshan Hu (728098), Mengmeng Li (400335), Xuehua Liu (728100), Chun Zhu (112527), Jingai Zhu (403132)   +8 more
core   +1 more source

Ionizable Lipid‐Dependent Optimization of Steroid Lipid Nanoparticles With Tunable Immunomodulatory Properties

Advanced Materials, EarlyView.
A novel engineering strategy that establishes material design principles for incorporating anti‐inflammatory steroids into lipid nanoparticles to reduce LNP‐induced inflammation while retaining mRNA delivery. Results are validated in vitro and in three animal models of inflammation and autoimmunity in vivo.
Ajay S. Thatte, Benjamin E. Nachod, Julia Baena, Jenna Muscat‐Rivera, Lesley Chaboub, Hannah C. Safford, Hannah C. Geisler, Hannah M. Yamagata, Melgious J. Y. Ang, Michael Kegel, Alexandre Poirier, Elaine R. Tong, Zhe Zhong, Qiangqiang Shi, Jinjin Wang, Elisa Battistini, Ye Zeng, Alex G. Hamilton, Kelsey L. Swingle, Drew Weissman, Jilian R. Melamed, Michael J. Mitchell   +21 more
wiley   +1 more source

Rapid Quantification of Bluetongue Virus-Neutralizing Antibodies Using Bioluminescent Reporter-Expressing Viruses

Vaccines
Bluetongue virus (BTV) is the causative agent of the significant livestock disease Bluetongue (BT), which causes severe economic losses associated with its considerable impact on the health and trade of ruminants. Background/Objectives: BTV infection and
Luis Jiménez-Cabello, Sergio Utrilla-Trigo, Eva Calvo-Pinilla, Aitor Nogales, Javier Ortego   +4 more
doaj   +1 more source

Spatial Control of Light‐Responsive Proteins and Optogenetics Within Hydrogels via Volumetric Bioprinting

Advanced Materials, EarlyView.
Spatiotemporal control over cell behavior within bioprinted constructs remains a key challenge in biofabrication. Volumetric bioprinting using multi‐wavelength light sources permits to activate light‐sensitive intracellular proteins as well as optogenetic gene and protein expression circuits in bioprinted cells, in addition to enabling fast printing of
Davide Ribezzi, Pere Català, Gabriel Größbacher, Paulina Nuñez Bernal, Olaf Nijssen, Sammy Florczak, Wilco Nijenhuis, Nuria Crusellas‐Villorbina, Bram Nijhoff, Paul Delrot, Jos Malda, Andreas Hierholzer, Martin Fussenegger, Lukas C. Kapitein, Riccardo Levato   +14 more
wiley   +1 more source

Critical parameters for robust Agrobacterium‐mediated transient transformation and quantitative promoter assays in Catharanthus roseus seedlings

Plant Direct
Agrobacterium‐mediated transient expression methods are widely used to study gene function in both model and non‐model plants. Using a dual‐luciferase assay, we quantified the effect of Agrobacterium‐infiltration parameters on the transient ...
Lauren F. Cole‐Osborn, Emma Meehan, Carolyn W. T. Lee‐Parsons   +2 more
doaj   +1 more source

Modification of H1N1 Influenza Luciferase Reporter Viruses Using StopGo Translation and/or Mouse-Adapted Mutations

Viruses
Reporter viruses are valuable tools for studying infections at the cellular level and in living animals. They also enable rapid, high-throughput antiviral drug screening and serological studies.
Po-Ling Chen, Guohua Yang, Chet Ojha, Balaji Banoth, Charles J. Russell   +4 more
doaj   +1 more source