Results 81 to 90 of about 325,683 (268)

Engineering recombinant replication-competent bluetongue viruses expressing reporter genes for in vitro and non-invasive in vivo studies

Microbiology Spectrum
Bluetongue virus (BTV) is the causative agent of the important livestock disease bluetongue (BT), which is transmitted via Culicoides bites. BT causes severe economic losses associated with its considerable impact on health and trade of animals.
Sergio Utrilla-Trigo, Luis Jiménez-Cabello, Alejandro Marín-López, Miguel Illescas-Amo, Germán Andrés, Eva Calvo-Pinilla, Gema Lorenzo, Piet A. van Rijn, Javier Ortego, Aitor Nogales   +9 more
doaj   +1 more source

Functional Characterisation and Analysis of the Soluble NKG2D Ligand Repertoire Detected in Umbilical Cord Blood Plasma

Frontiers in Immunology, 2018
We previously reported that cord blood plasma (CBP) contains significantly more soluble NKG2D ligands (sNKG2DLs), such as sMICB and sULBP1, than healthy adult plasma.
Steven T. Cox, Steven T. Cox, Robert Danby, Robert Danby, Diana Hernandez, Diana Hernandez, J. Alejandro Madrigal, J. Alejandro Madrigal, Aurore Saudemont, Aurore Saudemont   +9 more
doaj   +1 more source

Paternal Circadian Disruption Impairs Offspring Cognition via Sperm microRNAs

Advanced Science, EarlyView.
Paternal circadian disruption remodels the sperm small RNA payload, elevating miR‐92a‐3p/miR‐25‐3p levels and perturbing early embryonic gene regulatory programs. Microinjection experiments and single‐embryo transcriptomics reveal sex‐specific developmental vulnerabilities, ultimately impairing offspring hippocampal synaptic plasticity and cognition ...
Kexin Zou, Sisi Luo, Binliang Tang, Zhiqiang Liu, Yuchuan Zhou, Yicong Meng, Zhengmu Wu, Zheng Sun, Weihui Shi, Jianzhong Sheng, Chuanjin Yu, Zhenhua Li, Siyi Wei, Mo Zhang, Yujie Luo, Jing Yan, Xueyun Qin, Jiahang Mo, Chengliang Zhou, Jinsong Li, Hefeng Huang, Yifu Ding, Guolian Ding   +22 more
wiley   +1 more source

Relative luciferase activity.

, 2014
The light emission of luciferase was measured after addition of Luciferase Assay Reagent II, normalized against the protein content in samples transfected by pGL4-Irx3 (Irx), promoterless pGL4.12 (pB), pGL4-Irx3 plus increasing molar ratio of Nkx2.2 (N1,
András Dinnyés (4635640), Yu Gao (69071), Helen M. Byrne (224868), Bianka Juhász (660991), Krisztián A. Kovács (660993), Anna Lovrics (660990), István Bock (660992)   +6 more
core   +1 more source

Rapid Quantification of Bluetongue Virus-Neutralizing Antibodies Using Bioluminescent Reporter-Expressing Viruses

Vaccines
Bluetongue virus (BTV) is the causative agent of the significant livestock disease Bluetongue (BT), which causes severe economic losses associated with its considerable impact on the health and trade of ruminants. Background/Objectives: BTV infection and
Luis Jiménez-Cabello, Sergio Utrilla-Trigo, Eva Calvo-Pinilla, Aitor Nogales, Javier Ortego   +4 more
doaj   +1 more source

Chemical and biological investigation of the Agaricus silvaticus Schaeff ex. Secr

Mongolian Journal of Chemistry, 2014
Extract of Agaricus silvaticus Schaeff ex. Secr fungus have been known to have biological activities, including antibacterial, antiviral, anticancer, antioxidant, anticomplementary and immunostimulating effects.
L Munkhgerel, N Erdenechimeg, M Dumaa, G Zhang, P Odonmajig, D Regdel   +5 more
doaj   +1 more source

Zinc Exposure Causes Disulfidptosis to Induce Miscarriage by Up‐Regulating GATA1/METTL1/SLC7A11 Axis

Advanced Science, EarlyView.
Zn exposure up‐regulates GATA1, promoting GATA1‐mediated METTL1 and SLC7A11 transcription. It also enhances METTL1‐mediated m7G modification on SLC7A11 mRNA, increasing SLC7A11 mRNA stability. Ultimately, Zn exposure up‐regulates SLC7A11 at both transcriptional and post‐transcriptional levels, causing disulfidptosis. Knockdown of murine Slc7a11, Gata1,
Wenxin Huang, Yi Sun, Yanxin Wang, Haijun Yan, Xiaoping Yue, Weidong Wu, Xueyu Chen, Jinfang Zhang, Yanbing Lin, Qiong Lei, Nan Ji, Shuaishuai Xing, Liqin Zeng, Qingzheng Kang, Depeng Zhao, Geng Guo, Huidong Zhang   +16 more
wiley   +1 more source

Conditional circadian regulation of PHYTOCHROME A gene expression

, 2001
The phytochrome photoreceptors and the circadian clock control many of the same developmental processes, in all organs and throughout the growth of Arabidopsis plants. Phytochrome A (phyA) provides light input signals to entrain the circadian clock.
Nagy, F, Hall, Anthony, Kozma-Bognár, L, Tóth, R, Millar, A J; id_orcid   +4 more
core   +1 more source

Critical parameters for robust Agrobacterium‐mediated transient transformation and quantitative promoter assays in Catharanthus roseus seedlings

Plant Direct
Agrobacterium‐mediated transient expression methods are widely used to study gene function in both model and non‐model plants. Using a dual‐luciferase assay, we quantified the effect of Agrobacterium‐infiltration parameters on the transient ...
Lauren F. Cole‐Osborn, Emma Meehan, Carolyn W. T. Lee‐Parsons   +2 more
doaj   +1 more source

Modification of H1N1 Influenza Luciferase Reporter Viruses Using StopGo Translation and/or Mouse-Adapted Mutations

Viruses
Reporter viruses are valuable tools for studying infections at the cellular level and in living animals. They also enable rapid, high-throughput antiviral drug screening and serological studies.
Po-Ling Chen, Guohua Yang, Chet Ojha, Balaji Banoth, Charles J. Russell   +4 more
doaj   +1 more source