Results 251 to 260 of about 340,028 (311)
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Coelenterazine-dependent luciferases
Biochemistry (Moscow), 2015Bioluminescence is a widespread natural phenomenon. Luminous organisms are found among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies on bioluminescent systems of various organisms have revealed an interesting feature - the mechanisms underlying visible light emission are considerably different in representatives ...
S V, Markova, E S, Vysotski
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2006
Luciferase is the ideal reporter gene to provide temporal and spatial information on promoter activity in Arabidopsis and other eukaryotes; the noninvasive detection of luminescence and short half-life of luciferase activity allow repeated measurements of individual seedlings over several days to assay dynamic changes in gene expression.
Megan M, Southern +2 more
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Luciferase is the ideal reporter gene to provide temporal and spatial information on promoter activity in Arabidopsis and other eukaryotes; the noninvasive detection of luminescence and short half-life of luciferase activity allow repeated measurements of individual seedlings over several days to assay dynamic changes in gene expression.
Megan M, Southern +2 more
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Flavin binding by bacterial luciferase: Affinity chromatography of bacterial luciferase
Biochemical and Biophysical Research Communications, 1974Abstract Immobilized FMN covalently attached to Sepharose-6B-hexanoate binds bacterial luciferase. Immobilized flavin is also effective in its reduced form as a substrate in the light emitting reaction catalyzed by luciferase.
C A, Waters, J R, Murphy, J W, Hastings
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2006
This luciferase reporter assay provides a simple and highly sensitive method to determine the responsiveness of the Tet-system. It is quantitative, reproducible and easy to use in DT40 screens with both transient and stable transfections. The reaction catalyzed by firefly luciferase is the oxidation of luciferin in the presence of coenzyme A with ...
Frank R, Wettey, Antony P, Jackson
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This luciferase reporter assay provides a simple and highly sensitive method to determine the responsiveness of the Tet-system. It is quantitative, reproducible and easy to use in DT40 screens with both transient and stable transfections. The reaction catalyzed by firefly luciferase is the oxidation of luciferin in the presence of coenzyme A with ...
Frank R, Wettey, Antony P, Jackson
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Luciferases with Tunable Emission Wavelengths
Angewandte Chemie, 2017AbstractWe introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP‐tag or HaloTag7. Labeling of the self‐labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore.
Johnsson, Kai +9 more
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Measurement of Luciferase Rhythms
2014Firefly luciferase (LUC) is a sensitive and versatile reporter for the analysis of gene expression. Transgenic plants carrying CLOCK GENE promoter:LUC fusions can be assayed with high temporal resolution. LUC measurement is sensitive, noninvasive, and nondestructive and can be readily automated, greatly facilitating genetic studies.
C Robertson, McClung, Qiguang, Xie
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Beyond luciferase-luciferin system: Modification, improved imaging and biomedical application
Coordination Chemistry Reviews, 2023Fu Wang, Xiaorui Shi
exaly
Purification of Cypridina luciferase
Journal of Cellular and Comparative Physiology, 1951W D, McELROY, A M, CHASE
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Molecular cloning and expression in of a cDNA clone encoding luciferase of a firefly,
Biochimica Et Biophysica Acta Gene Regulatory Mechanisms, 1992Eiichi Nakano, H Tatsumi, Naoki Kajiyama
exaly