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Crystalline firefly luciferase
Biochimica et Biophysica Acta, 1956Abstract 1. 1. The method for purification and crystallization of firefly luciferase is described. 2. 2. The crystalline enzyme is apparently homogenous as evidenced by ultracentrifugation a and electrophoretic determinations. The molecular weight is probably around 100,000 and the isoelectric point between pH 6.2 and 6.3. 3. 3.
A A, GREEN, W D, MCELROY
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Expert Opinion on Drug Discovery, 2010
By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body.This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied
Yoshihiro, Nakajima, Yoshihiro, Ohmiya
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By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body.This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied
Yoshihiro, Nakajima, Yoshihiro, Ohmiya
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Journal of Photochemistry and Photobiology B: Biology, 2010
Firefly luciferase (Luc) is the most studied of the luciferase enzymes and the mechanism and kinetics of the reactions catalyzed by this enzyme have been relatively well characterized. Luc catalyzes the bioluminescent reaction involving firefly luciferin (D-LH(2)), adenosine triphosphate (ATP), magnesium ion and molecular oxygen with the formation of ...
João M. M. Leitão +1 more
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Firefly luciferase (Luc) is the most studied of the luciferase enzymes and the mechanism and kinetics of the reactions catalyzed by this enzyme have been relatively well characterized. Luc catalyzes the bioluminescent reaction involving firefly luciferin (D-LH(2)), adenosine triphosphate (ATP), magnesium ion and molecular oxygen with the formation of ...
João M. M. Leitão +1 more
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Coelenterazine-dependent luciferases
Biochemistry (Moscow), 2015Bioluminescence is a widespread natural phenomenon. Luminous organisms are found among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies on bioluminescent systems of various organisms have revealed an interesting feature - the mechanisms underlying visible light emission are considerably different in representatives ...
S V, Markova, E S, Vysotski
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Multiplex Hextuple Luciferase Assaying
2022We recently expanded the commonly used dual luciferase assaying method toward multiplex hextuple luciferase assaying, allowing monitoring the activity of five experimental pathways against one control at the same time. In doing so, while our expanded assay utilizes a total of six orthogonal luciferases instead of two, this assay, conveniently, still ...
Sarrion-Perdigones, Alejandro +5 more
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Cold Spring Harbor Protocols, 2010
INTRODUCTIONWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays. The reporter protein’
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INTRODUCTIONWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays. The reporter protein’
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2006
This luciferase reporter assay provides a simple and highly sensitive method to determine the responsiveness of the Tet-system. It is quantitative, reproducible and easy to use in DT40 screens with both transient and stable transfections. The reaction catalyzed by firefly luciferase is the oxidation of luciferin in the presence of coenzyme A with ...
Frank R, Wettey, Antony P, Jackson
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This luciferase reporter assay provides a simple and highly sensitive method to determine the responsiveness of the Tet-system. It is quantitative, reproducible and easy to use in DT40 screens with both transient and stable transfections. The reaction catalyzed by firefly luciferase is the oxidation of luciferin in the presence of coenzyme A with ...
Frank R, Wettey, Antony P, Jackson
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Resveratrol inhibits firefly luciferase
Biochemical and Biophysical Research Communications, 2006The potential therapeutic value of resveratrol in age-related disease settings including cancer, diabetes, and Alzheimer's has emerged from a rapidly growing body of experimental evidence. Protection from oxidative stress appears to be a common feature of resveratrol that may be mediated through SirT1, though more specific molecular mechanisms by which
Adel, Bakhtiarova +6 more
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Flavin binding by bacterial luciferase: Affinity chromatography of bacterial luciferase
Biochemical and Biophysical Research Communications, 1974Abstract Immobilized FMN covalently attached to Sepharose-6B-hexanoate binds bacterial luciferase. Immobilized flavin is also effective in its reduced form as a substrate in the light emitting reaction catalyzed by luciferase.
C A, Waters, J R, Murphy, J W, Hastings
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