Results 261 to 270 of about 14,115 (284)
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Mechanical Control of Renilla Luciferase
Journal of the American Chemical Society, 2013We report experiments where the activity of the enzyme luciferase from Renilla reniformis is controlled through a DNA spring attached to the enzyme. In the wake of previous work on kinases, these results establish that mechanical stress applied through the DNA springs is indeed a general method for the artificial control of enzymes, and for the ...
Chiao-Yu, Tseng, Giovanni, Zocchi
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Fabrication of a New Lineage of Artificial Luciferases from Natural Luciferase Pools
ACS Combinatorial Science, 2017The fabrication of artificial luciferases (ALucs) with unique optical properties has a fundamental impact on bioassays and molecular imaging. In this study, we developed a new lineage of ALucs with unique substrate preferences by extracting consensus amino acids from the alignment of 25 copepod luciferase sequences available in natural luciferase pools.
Sung Bae Kim +3 more
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Dual Luciferase Assay for Secreted Luciferases Based onGaussiaand NanoLuc
ASSAY and Drug Development Technologies, 2013Just recently, NanoLuc, a new engineered luciferase based on the small subunit of the luciferase from Oplophorus gracilirostris was introduced. Like the luciferase from Gaussia princeps, this luciferase is secreted into the medium. Both luciferases are the smallest and brightest luciferases known and well-suited for reporter assays. In our experiments,
Kerstin, Heise +4 more
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The Split Luciferase Complementation Assay
2010A split luciferase complementation assay to study protein-protein interactions within Arabidopsis protoplasts in 96-well plates is described in this protocol. Two proteins of interest, a bait and prey, which are genetically fused to amino- and carboxy-terminal fragments of Renilla luciferase, are transiently expressed in protoplasts.
Naohiro, Kato, Jason, Jones
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1955
Publisher Summary Bacterial luciferase is a flavoprotein which catalyzes the oxidation of reduced DPN by numerous oxidants such as FMN, ferricyanide, quinones, and various dyes. In the presence of FMN and a long-chain fatty aldehyde the oxidation of DPNH is accompanied by light emission.
Arda A. Green, William D. McElroy
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Publisher Summary Bacterial luciferase is a flavoprotein which catalyzes the oxidation of reduced DPN by numerous oxidants such as FMN, ferricyanide, quinones, and various dyes. In the presence of FMN and a long-chain fatty aldehyde the oxidation of DPNH is accompanied by light emission.
Arda A. Green, William D. McElroy
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Purification of Cypridina luciferase
Journal of Cellular and Comparative Physiology, 1951W D, McELROY, A M, CHASE
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Structurally distinct bacterial luciferases
Biochemistry, 1969J W, Hastings +5 more
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