Results 171 to 180 of about 67,421 (349)

Bispecific Ligand‐Based EphB4 CAR‐T Cells Generated Using Ephrin‐B2 Show Potent Antitumor Activity Against Lung Adenocarcinoma

Cancer Science, EarlyView.
EphB4 and EphA2 positivity in lung adenocarcinoma samples was high. Ligand‐based EphB4 CAR‐T cells exhibit bispecificity, targeting both EphB4 and EphA2. EphB4 CAR‐T cells exert significant antitumor effects against lung adenocarcinoma. ABSTRACT Chimeric antigen receptor (CAR)‐T cells generated using ephrin‐B2, the natural ligand for ephrin type‐B ...
Hirotaka Kumeda, Koichi Hirabayashi, Shuji Mishima, Kotaro Morita, Yu Furui, Marina Fujioka, Tomoyuki Nakajima, Miyuki Tanaka, Yoshinori Sato, Takeshi Uehara, Shigeki Yagyu, Kimihiro Shimizu, Yozo Nakazawa   +12 more
wiley   +1 more source

Decreased Expression of FAT4 Promotes Multiple Myeloma Proliferation and Migration by Targeting the Hippo/YAP Pathway

Cancer Science, EarlyView.
This study provides the first comprehensive characterization of the functional impact, and mechanistic role of FAT4 in MM. Our findings highlight the tumor‐suppressive role of FAT4 in MM and uncover a novel mechanism by which FAT4 regulates the Hippo/YAP pathway.
Lina Zhang, Na Shen, Xin Cheng, Qinglin Shi, Lijuan Chen, Xuxing Shen   +5 more
wiley   +1 more source

AMSC‐sEVs Ameliorated Crohn's Disease by Inhibiting Macrophage‐Myofibroblast Transition Through the Delivery of MFGE8

Cell Proliferation, EarlyView.
Our study demonstrated that TGF‐β1 activated MAPK signalling pathway and promoted the MMT and secretion of CCL17 under CD‐associated intestinal inflammatory conditions. AMSC‐sEVs can deliver MFGE8, which inhibits MMT and CCL17‐mediated Treg recruitment by suppressing the MAPK signalling pathway, thereby alleviating intestinal fibrosis.
Minghao Xie, Qiang Liu, Zhizhong Xiong, Jian Li, Ruiri Jin, Lei Lian, Zhengrong Li   +6 more
wiley   +1 more source

Determination of ATP Content in Cells

Cell Proliferation, EarlyView.
The overlap HPLC chromatograms of ATP standard (red) and ATP in cells (blue). Poroshell 120 EC‐C18 (3 × 150 mm, 2.7 μm), 25°C, pH 6.8 buffer solution of 0.05 mol/L KH2PO4 ‐ 0.05 mol/L K2HPO4 (V:V = 1:1) as mobile phase, 0.6 mL/min, 254 nm.
Boqiang Fu, Wenfeng Huang, Yingying Liu, Lei Wang, Yuan Liu, Jiani Cao, Wenjuan Duan, Aijin Ma, Hongling Zhao, Shuaishuai Niu, Shijun Hu, Qiyuan Li, Yong Zhang, Yaojin Peng, Xiaoyou Yu, Junying Yu, Jun Wei, Yu Zhang, Guoqiang Hua, Xin Liu, Changlin Wang, Tao Na, Yang Zhao, Jiaxi Zhou, Peng Xiang, Zhihong Wu, Qubo Chen, Peijun Zhai, Hengjun Gao, Jie Hao, Tongbiao Zhao, Jing Wang   +31 more
wiley   +1 more source

Problem areas in the use of the firefly luciferase assay for bacterial detection [PDF]

By purifying the firefly luciferase extract and adding all necessary chemicals but ATP in excess, an assay for ATP was performed by measuring the amount of light produced when a sample containing soluble ATP is added to the luciferase reaction mixture ...
Chappelle, E. W., Curtis, C. A., Knust, E. A., Picciolo, G. L., Tuttle, S. A.   +4 more
core   +1 more source

Orthogonal Luciferase-Luciferin Pairs for Bioluminescence Imaging.

Journal of the American Chemical Society, 2017
Krysten A. Jones, William B. Porterfield, Colin M Rathbun, David Mccutcheon, M. A. Paley, Jennifer A. Prescher   +5 more
semanticscholar   +1 more source

Spatiotemporal dynamics of β‐arrestin‐mediated Src activation in 5‐HT7 receptor signaling pathway

The FEBS Journal, EarlyView.
GPCRs induce distinct cellular responses via G protein‐ or β‐arrestin‐mediated signaling pathways. This study revealed that β‐arrestin‐biased 5‐HT7R ligand induces slow, sustained Src activation, contrasting with transient G protein‐mediated activation.
Hyunbin Kim, Yong Woo Lee, Huimin Lee, Eunseo Park, Jieon Lee, Hae Nim Lee, Kyeong‐Man Kim, Hyunah Choo, Jihye Seong   +8 more
wiley   +1 more source

Bioluminescence of the heterotrophic dinoflagellate Polykrikos kofoidii Chatton 1914 (Dinophyceae). [PDF]

J Phycol
Latz MI, Deheyn DD, Sprecher BN.
europepmc   +1 more source

The HaMYB22–HaGST3.2 module mediates salt stress response in sunflower

Journal of Integrative Plant Biology, EarlyView.
The transcription factors HaMYB22, HaMYB120, and HaMYB121 cooperatively activate the transcription of the glutathione S‐transferase gene HaGST3.2, thereby facilitating the removal of reactive oxygen species induced by salt stress in sunflowers and improving their salt tolerance.
Siqi Zhang, Yuliang Han, Qixiu Huang, Weijun Guo, Shurui Dong, Xinxin Li, Qian Zhang, Juncheng Zhang, Yijun Meng, Zhonghua Lei, Maohong Cai, Tao Chen   +11 more
wiley   +1 more source

A practical guideline for in vivo bioluminescence imaging. [PDF]

Mol Cells
Lee H, Chae JS, Park HW.
europepmc   +1 more source